getogrow
Well-Known Member
i know you aint sayin j roc is an embarrassment to Canada knowmimsayin ?! i was getting changed!!!
Clone or a clone of a clone
- Thread starter BodegaBud
- Start date
sf_frankie
Well-Known Member
He’s pullin his goalie!
HydroKid239
Well-Known Member
+1 for genetics remaining intact
Kassiopeija
Well-Known Member
Here's a new study on that topic, it investigated genetic aberrations from 9 samples from the top, middle & bottom regions of a 1.5yr old mother-plant:
The most mutations occur at the top, while the center is more stable.
"Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content."
"5 CONCLUSION
The findings in this study demonstrate that genetic diversity exists with a single cannabis plant and the genetic mosaicism hypothesis applies to cannabis. This study is the first to investigate the existence of this phenomenon in cannabis plants and the potential consequences of accumulating somatic mutations in an artificially prolonged annual species. As cannabis normally lives for ∼3–6 mo, this process likely enables an unknown but manageable amount of somatic mutations to accumulate. Currently, somatic mutations in plants have many uncertainties remaining, but as a result of modern genetic technologies and more affordable WGS, there have been more contributions with higher degrees of accuracy and precision on this topic. From a practical standpoint, this significantly benefits the cannabis industry as understanding this phenomenon will help establish best practices for maintaining mother plants to minimize, slow, or prevent the accumulation of mutations. Based on these data, we advocate replacing mother plants using cuttings from the basal portion of the plant and discourage excessively extending the life of a mother plant. Additionally, important genetics should be preserved using cryopreservation techniques where the original genetic profile can be maintained and accessed indefinitely (Uchendu et al., 2019). The research here provides a concrete basis for cannabis mutation research. However, the current study lacked different cultivars, generational data, mutation rates and multiple biological replicates. Thus, future research will be necessary to enhance and solidify our understanding of somatic mutations and the mutagenic potential within a cannabis mother plant."
The most mutations occur at the top, while the center is more stable.
"Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content."
"5 CONCLUSION
The findings in this study demonstrate that genetic diversity exists with a single cannabis plant and the genetic mosaicism hypothesis applies to cannabis. This study is the first to investigate the existence of this phenomenon in cannabis plants and the potential consequences of accumulating somatic mutations in an artificially prolonged annual species. As cannabis normally lives for ∼3–6 mo, this process likely enables an unknown but manageable amount of somatic mutations to accumulate. Currently, somatic mutations in plants have many uncertainties remaining, but as a result of modern genetic technologies and more affordable WGS, there have been more contributions with higher degrees of accuracy and precision on this topic. From a practical standpoint, this significantly benefits the cannabis industry as understanding this phenomenon will help establish best practices for maintaining mother plants to minimize, slow, or prevent the accumulation of mutations. Based on these data, we advocate replacing mother plants using cuttings from the basal portion of the plant and discourage excessively extending the life of a mother plant. Additionally, important genetics should be preserved using cryopreservation techniques where the original genetic profile can be maintained and accessed indefinitely (Uchendu et al., 2019). The research here provides a concrete basis for cannabis mutation research. However, the current study lacked different cultivars, generational data, mutation rates and multiple biological replicates. Thus, future research will be necessary to enhance and solidify our understanding of somatic mutations and the mutagenic potential within a cannabis mother plant."
twentyeight.threefive
Well-Known Member
You should have made your username "CutNPaste".
bk78
Well-Known Member
Crazy how there are mother plants decades old already
Wastei
Well-Known Member
Why do you keep posting trash without real substance? The study is from tissue samples from 3 different locations from one mother, I repeat ONE(!) how do you think this is relevant or even usable info?
Kassiopeija
Well-Known Member
yeah really hard to understand
just read the study and spare us your wimpy drama
when you don't understand how science works it's on your own
DoubleAtotheRON
Well-Known Member
What's up with the 8.4 C?
Moflow
Well-Known Member
That's the temperature with the doors open to feed them, it's generally around 15°C - 59f when doors are closed this time of year.
DoubleAtotheRON
Well-Known Member
Wow... that seems pretty cool for veg. Seems to be working for you tho!.. Im about to start my 4th year with a pheno.... always a great producer, and I've made hundreds upon hundreds of plants from her.
Moflow
Well-Known Member
They're in flower about 12 days now
Popop
Well-Known Member
Why so cold?
Moflow
Well-Known Member
I like to experiment.
I suppose I just like to show that's it's possible to grow in cooler temperatures than the majority of people use .
I realise cold growing has it's limitations but can still be done successfully.
That particular grow area is under ~ 150 watts of led in a 4 x 4.
Kassiopeija
Well-Known Member
One thing to realize which the study just hinted at is that most mutations that occur are rather negative than positive. Because they are strictly random...It's why breeding gets better with the amount of rigorous selecting it undertakes.
Also, plants have a large genome with important fields hard-conserved which is only fair to mention the study itself closes by saying more research needs to be done on that topic.
Still, the goal it set itself has been reached - to confirm this "moasic gene theory" and falsify the "anecdotal" (=bro'science) claimed total preservation of genetics. And while the retards only laugh, it gave helpful hints how to minimize these losses
Also, plants have a large genome with important fields hard-conserved which is only fair to mention the study itself closes by saying more research needs to be done on that topic.
Still, the goal it set itself has been reached - to confirm this "moasic gene theory" and falsify the "anecdotal" (=bro'science) claimed total preservation of genetics. And while the retards only laugh, it gave helpful hints how to minimize these losses
twentyeight.threefive
Well-Known Member
The goal it set itself was reached? With a one plant sample. Very scientific.One thing to realize which the study just hinted at is that most mutations that occur are rather negative than positive. Because they are strictly random...It's why breeding gets better with the amount of rigorous selecting it undertakes.
Also, plants have a large genome with important fields hard-conserved which is only fair to mention the study itself closes by saying more research needs to be done on that topic.
Still, the goal it set itself has been reached - to confirm this "moasic gene theory" and falsify the "anecdotal" (=bro'science) claimed total preservation of genetics. And while the retards only laugh, it gave helpful hints how to minimize these losses
I don't think the word retard is appropriate.
a mongo frog
Well-Known Member
Definitely not appropriate. Apologize immediately please.