Clone or a clone of a clone

Kassiopeija

Kassiopeija

Well-Known Member
Here's a new study on that topic, it investigated genetic aberrations from 9 samples from the top, middle & bottom regions of a 1.5yr old mother-plant:
Screenshot_20220402-214022~2.png
The most mutations occur at the top, while the center is more stable.

"Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content."

"5 CONCLUSION
The findings in this study demonstrate that genetic diversity exists with a single cannabis plant and the genetic mosaicism hypothesis applies to cannabis. This study is the first to investigate the existence of this phenomenon in cannabis plants and the potential consequences of accumulating somatic mutations in an artificially prolonged annual species. As cannabis normally lives for ∼3–6 mo, this process likely enables an unknown but manageable amount of somatic mutations to accumulate. Currently, somatic mutations in plants have many uncertainties remaining, but as a result of modern genetic technologies and more affordable WGS, there have been more contributions with higher degrees of accuracy and precision on this topic. From a practical standpoint, this significantly benefits the cannabis industry as understanding this phenomenon will help establish best practices for maintaining mother plants to minimize, slow, or prevent the accumulation of mutations. Based on these data, we advocate replacing mother plants using cuttings from the basal portion of the plant and discourage excessively extending the life of a mother plant. Additionally, important genetics should be preserved using cryopreservation techniques where the original genetic profile can be maintained and accessed indefinitely (Uchendu et al., 2019). The research here provides a concrete basis for cannabis mutation research. However, the current study lacked different cultivars, generational data, mutation rates and multiple biological replicates. Thus, future research will be necessary to enhance and solidify our understanding of somatic mutations and the mutagenic potential within a cannabis mother plant."

 
twentyeight.threefive

twentyeight.threefive

Well-Known Member
Kassiopeija said:
Here's a new study on that topic, it investigated genetic aberrations from 9 samples from the top, middle & bottom regions of a 1.5yr old mother-plant:
View attachment 5112198
The most mutations occur at the top, while the center is more stable.

"Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content."

"5 CONCLUSION
The findings in this study demonstrate that genetic diversity exists with a single cannabis plant and the genetic mosaicism hypothesis applies to cannabis. This study is the first to investigate the existence of this phenomenon in cannabis plants and the potential consequences of accumulating somatic mutations in an artificially prolonged annual species. As cannabis normally lives for ∼3–6 mo, this process likely enables an unknown but manageable amount of somatic mutations to accumulate. Currently, somatic mutations in plants have many uncertainties remaining, but as a result of modern genetic technologies and more affordable WGS, there have been more contributions with higher degrees of accuracy and precision on this topic. From a practical standpoint, this significantly benefits the cannabis industry as understanding this phenomenon will help establish best practices for maintaining mother plants to minimize, slow, or prevent the accumulation of mutations. Based on these data, we advocate replacing mother plants using cuttings from the basal portion of the plant and discourage excessively extending the life of a mother plant. Additionally, important genetics should be preserved using cryopreservation techniques where the original genetic profile can be maintained and accessed indefinitely (Uchendu et al., 2019). The research here provides a concrete basis for cannabis mutation research. However, the current study lacked different cultivars, generational data, mutation rates and multiple biological replicates. Thus, future research will be necessary to enhance and solidify our understanding of somatic mutations and the mutagenic potential within a cannabis mother plant."

Click to expand...
You should have made your username "CutNPaste".
 
bk78

bk78

Well-Known Member
Kassiopeija said:
Here's a new study on that topic, it investigated genetic aberrations from 9 samples from the top, middle & bottom regions of a 1.5yr old mother-plant:
View attachment 5112198
The most mutations occur at the top, while the center is more stable.

"Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content."

"5 CONCLUSION
The findings in this study demonstrate that genetic diversity exists with a single cannabis plant and the genetic mosaicism hypothesis applies to cannabis. This study is the first to investigate the existence of this phenomenon in cannabis plants and the potential consequences of accumulating somatic mutations in an artificially prolonged annual species. As cannabis normally lives for ∼3–6 mo, this process likely enables an unknown but manageable amount of somatic mutations to accumulate. Currently, somatic mutations in plants have many uncertainties remaining, but as a result of modern genetic technologies and more affordable WGS, there have been more contributions with higher degrees of accuracy and precision on this topic. From a practical standpoint, this significantly benefits the cannabis industry as understanding this phenomenon will help establish best practices for maintaining mother plants to minimize, slow, or prevent the accumulation of mutations. Based on these data, we advocate replacing mother plants using cuttings from the basal portion of the plant and discourage excessively extending the life of a mother plant. Additionally, important genetics should be preserved using cryopreservation techniques where the original genetic profile can be maintained and accessed indefinitely (Uchendu et al., 2019). The research here provides a concrete basis for cannabis mutation research. However, the current study lacked different cultivars, generational data, mutation rates and multiple biological replicates. Thus, future research will be necessary to enhance and solidify our understanding of somatic mutations and the mutagenic potential within a cannabis mother plant."

Click to expand...

Crazy how there are mother plants decades old already
 
Wastei

Wastei

Well-Known Member
Kassiopeija said:
Here's a new study on that topic, it investigated genetic aberrations from 9 samples from the top, middle & bottom regions of a 1.5yr old mother-plant:
View attachment 5112198
The most mutations occur at the top, while the center is more stable.

"Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content."

"5 CONCLUSION
The findings in this study demonstrate that genetic diversity exists with a single cannabis plant and the genetic mosaicism hypothesis applies to cannabis. This study is the first to investigate the existence of this phenomenon in cannabis plants and the potential consequences of accumulating somatic mutations in an artificially prolonged annual species. As cannabis normally lives for ∼3–6 mo, this process likely enables an unknown but manageable amount of somatic mutations to accumulate. Currently, somatic mutations in plants have many uncertainties remaining, but as a result of modern genetic technologies and more affordable WGS, there have been more contributions with higher degrees of accuracy and precision on this topic. From a practical standpoint, this significantly benefits the cannabis industry as understanding this phenomenon will help establish best practices for maintaining mother plants to minimize, slow, or prevent the accumulation of mutations. Based on these data, we advocate replacing mother plants using cuttings from the basal portion of the plant and discourage excessively extending the life of a mother plant. Additionally, important genetics should be preserved using cryopreservation techniques where the original genetic profile can be maintained and accessed indefinitely (Uchendu et al., 2019). The research here provides a concrete basis for cannabis mutation research. However, the current study lacked different cultivars, generational data, mutation rates and multiple biological replicates. Thus, future research will be necessary to enhance and solidify our understanding of somatic mutations and the mutagenic potential within a cannabis mother plant."

Click to expand...
Why do you keep posting trash without real substance? The study is from tissue samples from 3 different locations from one mother, I repeat ONE(!) how do you think this is relevant or even usable info?
 
Kassiopeija

Kassiopeija

Well-Known Member
Wastei said:
Why do you keep posting trash without real substance? The study is from tissue samples from 3 different locations from one mother, I repeat ONE(!) how do you think this is relevant or even usable info?
Click to expand...
yeah really hard to understand :wall:
just read the study and spare us your wimpy drama
when you don't understand how science works it's on your own
 
Moflow

Moflow

Well-Known Member
Please feel free to laugh.
This a clone of a clone ad infinitum from a seed ~ 14 yrs ago. Thousands of clones have been taken so far and thousands more will be taken in the years to come lol
I do like to treat them rough too, no molycoddling in my growing.
20220331_184659.jpg20220331_184810.jpg
Hahaha
 
DoubleAtotheRON

DoubleAtotheRON

Well-Known Member
Moflow said:
That's the temperature with the doors open to feed them, it's generally around 15°C - 59f when doors are closed this time of year.
Click to expand...
Wow... that seems pretty cool for veg. Seems to be working for you tho!.. Im about to start my 4th year with a pheno.... always a great producer, and I've made hundreds upon hundreds of plants from her.
 
Kassiopeija

Kassiopeija

Well-Known Member
One thing to realize which the study just hinted at is that most mutations that occur are rather negative than positive. Because they are strictly random...It's why breeding gets better with the amount of rigorous selecting it undertakes.

Also, plants have a large genome with important fields hard-conserved which is only fair to mention the study itself closes by saying more research needs to be done on that topic.

Still, the goal it set itself has been reached - to confirm this "moasic gene theory" and falsify the "anecdotal" (=bro'science) claimed total preservation of genetics. And while the retards only laugh, it gave helpful hints how to minimize these losses ;)
 
twentyeight.threefive

twentyeight.threefive

Well-Known Member
Kassiopeija said:
One thing to realize which the study just hinted at is that most mutations that occur are rather negative than positive. Because they are strictly random...It's why breeding gets better with the amount of rigorous selecting it undertakes.

Also, plants have a large genome with important fields hard-conserved which is only fair to mention the study itself closes by saying more research needs to be done on that topic.

Still, the goal it set itself has been reached - to confirm this "moasic gene theory" and falsify the "anecdotal" (=bro'science) claimed total preservation of genetics. And while the retards only laugh, it gave helpful hints how to minimize these losses ;)
Click to expand...
The goal it set itself was reached? With a one plant sample. Very scientific.

I don't think the word retard is appropriate.
 
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