Anybody growing mushrooms willing to talk???

Thundercat

Well-Known Member
Yeah, man, I need to get a flow hood. There are so many things that I want to do that I don't think would be very successful without it.
Oh yeah flow hood was key to the success we had. We started with a little 12inchx18inch but eventually got a deal on a 3ftx8ft unit that was awesome. Really for smaller setups a nice 2ftx2ft would work really well, thats what a buddy of mine uses and has great success.
 

canndo

Well-Known Member
I'd like to use alot more LC when inoculating but i don't wanna mess up the moisture content of the grain jars. I usually use 2 cc's of LC per quart jar and they're usually done in 10 to 14 days. I'd love to use more especially because i make jars of LC and always have extra that goes to waste. I'm sure they would colonize a lot faster.

Ok, I've Aldo enriched my lc with peptone and a dash of dextrose.

It usually has the effect of making good and gobs of mycelium. Then I will spin up the jar and draw 60 ml. Then I set the syringe needle side down and let it sit.

You get hyper concentrated mycelium in the first 20 MLS. Then just inject that, or a portion of it. In 84 degrees I get full colonization in 5 days. But of course it depends on your pheno or strain.
 

canndo

Well-Known Member
I've always been a bigger fan of agar transfers then LC. I feel like its easier to see contams on agar, and it colonizes so fast in grain bags.

With the level of production we were doing I actually did agar tranfers into 3lb MASTER grain bags. Then I would grain to grain transfer that into 6-8 larger 7lb grain bags once it was colonized.

I have always vasilated etween the two techniques. If I am not ABSOLUTELY certain my lc is not contaminated I test it on a dish... but then I have risked contamination in the lc again.

Three tiny cubes of agar, for me, only pushes back colonization by some three days. I find that mycelium will never migrate to the grain until it has covered the cube, hence the tiny size.

I never use lc unless it is from a Clone somewhere in the past. Never saw the point in multi spore lc.
 

canndo

Well-Known Member
Oh yeah flow hood was key to the success we had. We started with a little 12inchx18inch but eventually got a deal on a 3ftx8ft unit that was awesome. Really for smaller setups a nice 2ftx2ft would work really well, thats what a buddy of mine uses and has great success.

Interesting, I rarely use my hood anymore. Save for agar isolation, every procedure is done in open air. It's all needle to port. Fresh needles each time.
 

Thundercat

Well-Known Member
I have always vasilated etween the two techniques. If I am not ABSOLUTELY certain my lc is not contaminated I test it on a dish... but then I have risked contamination in the lc again.

Three tiny cubes of agar, for me, only pushes back colonization by some three days. I find that mycelium will never migrate to the grain until it has covered the cube, hence the tiny size.

I never use lc unless it is from a Clone somewhere in the past. Never saw the point in multi spore lc.
I started with little cubes of agar and little jars of grain that usually took 10ish days to colonize. When I moved to the Master bag system I started using a half a plate of agar in each bag, because it was fast and easy to make enough agar plates each week. I'd have full colonization of the 3lbs in a week or less, usually without shaking(other then at inoculation). Then G2G and another 7-10 days for the big bags to be ready, usually without a shake as well.
 

canndo

Well-Known Member
I have always vasilated etween the two techniques. If I am not ABSOLUTELY certain my lc is not contaminated I test it on a dish... but then I have risked contamination in the lc again.

Three tiny cubes of agar, for me, only pushes back colonization by some three days. I find that mycelium will never migrate to the grain until it has covered the cube, hence the tiny size.

I never use lc unless it is from a Clone somewhere in the past. Never saw the point in multi spore lc.
I have to Amend that. I have perpetually diluted a spore solution till I get a spore or two per mil and inject two or three mills into a small batch of lc. That limits my phenos and after fruiting I don't get all those freaks, late fruits and poor pin sets.

I've isolated two that do not produce spores that way. I thought at first that it was just early scencience but apparently not. I HATE all that purple brown stain on the caps and substrate.
 

Thundercat

Well-Known Member
Interesting, I rarely use my hood anymore. Save for agar isolation, every procedure is done in open air. It's all needle to port. Fresh needles each time.
I did all my agar work, spore work, and even G2G infront of the hood. I was working a lot of bags and plates a week and couldn't afford a failure on my end. The only things we didn't do infront of the hood was building tubs, and harvesting. We also had the old smaller flow hood filter set up on the intake to the lab so the air coming in was filtered and we had posative pressure on the room too.
 

canndo

Well-Known Member
I started with little cubes of agar and little jars of grain that usually took 10ish days to colonize. When I moved to the Master bag system I started using a half a plate of agar in each bag, because it was fast and easy to make enough agar plates each week. I'd have full colonization of the 3lbs in a week or less, usually without shaking(other then at inoculation). Then G2G and another 7-10 days for the big bags to be ready, usually without a shake as well.

I don't have the nerve to do g2g, even before a hood.
 

canndo

Well-Known Member
I did all my agar work, spore work, and even G2G infront of the hood. I was working a lot of bags and plates a week and couldn't afford a failure on my end. The only things we didn't do infront of the hood was building tubs, and harvesting. We also had the old smaller flow hood filter set up on the intake to the lab so the air coming in was filtered and we had posative pressure on the room too.
I got very lucky. I worked for an electronics assembly house. They damaged a top flow 4 ft table and were about to toss it so I got it for free and only had to replace the damaged element.. but it is loud.
 

Thundercat

Well-Known Member
I don't have the nerve to do g2g, even before a hood.
Oh man, it was so easy. I just poured grain from one bag into the others, or use a measuring cup that you alcohol between batches. I tried to pour the same amount in each, then heat sealed them and shook the hell out of them to spread the grain and moisture in the bag around. Then label and stack on the shelf for a week till its done.
 

7L!fTeD24

Well-Known Member
I have always vasilated etween the two techniques. If I am not ABSOLUTELY certain my lc is not contaminated I test it on a dish... but then I have risked contamination in the lc again.

Three tiny cubes of agar, for me, only pushes back colonization by some three days. I find that mycelium will never migrate to the grain until it has covered the cube, hence the tiny size.

I never use lc unless it is from a Clone somewhere in the past. Never saw the point in multi spore lc.
That's exactly why I don't test my LC before Inoculating my jars Because that's risking Contamination also.
 

7L!fTeD24

Well-Known Member
Sounds like a flow hood We'll be my next buy. I just don't want to have to spend $1000+ on it. All I really need is a small one, Any suggestions where I can get a Smaller one That's pretty cheap but not cheaply made.
 

Thundercat

Well-Known Member
Sounds like a flow hood We'll be my next buy. I just don't want to have to spend $1000+ on it. All I really need is a small one, Any suggestions where I can get a Smaller one That's pretty cheap but not cheaply made.
Theres a bunch of options just with a google search. Seems like quite a few under 1k. I would definitely get at least a 2'x2' for ease of working. Its tough to keep eveything in the air flow on real small hoods.
 

Aeroknow

Well-Known Member
Theres a bunch of options just with a google search. Seems like quite a few under 1k. I would definitely get at least a 2'x2' for ease of working. Its tough to keep eveything in the air flow on real small hoods.
I picked up a 24x48 a couple months ago and already wish i had another one next to it. Would be a straight up assembly line going on.
Sounds like a flow hood We'll be my next buy. I just don't want to have to spend $1000+ on it. All I really need is a small one, Any suggestions where I can get a Smaller one That's pretty cheap but not cheaply made.
I built this one for around 3 hundred. Has a really nice filter on it compared to the bigger complete units you see for sale online. It’s an 14”x24”. I had quickly outgrew it right after i assembled it. Never did make the pre filter box for up top.
image.jpg
 
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Thundercat

Well-Known Member
I picked up a 24x48 a couple months ago and already wish i had another one next to it. Would be a straight up assembly line going on.

I built this one for around 3 hundred. Has a really nice filter on it compared to the bigger complete units you see for sale online. It’s an 18”x24”. I had quickly outgrew it right after i assembled it. Never did make the pre filter box for up top.
View attachment 5339634

Looks like a nice build. You could position that one next to your 2x4 and get a little extra space :).

When we went from 12"x18" up the the 3ftx8ft it was like a dream come true. Thats about the same time I got a lab assistant. I did the culture work on one side, and sealed the bags, and he labeled them, shook them up, and shelved them.
 

natureboygrower

Well-Known Member
I picked up a 24x48 a couple months ago and already wish i had another one next to it. Would be a straight up assembly line going on.

I built this one for around 3 hundred. Has a really nice filter on it compared to the bigger complete units you see for sale online. It’s an 18”x24”. I had quickly outgrew it right after i assembled it. Never did make the pre filter box for up top.
View attachment 5339634
Haha, dude I thought you used an AC sleeve or similar til I saw the unpainted wood! Clean build
 

Aeroknow

Well-Known Member
Sure is. Urethane fortified latex should be standard on interior trim imo.
Are the filters you bought available at a place like mycology-supply?
I paint all trim with that stuff now. Painted my kitchen cabinets with it too.
I bought this one off ebay. And it’s a 14x24 not 18x24. Duh lol. I corrected my post above.
 
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