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by the way, the best way to dispurse those clumps is to use an ultrasonic cleaner and imerse the syring in that. A few minutes to hapf an hour works just fine. I always use one with syringes but it has been a while

You can't do sector selection if you swab the dish. Spores need to find complementary spores and make clamp connections. The closer the spores are together the quicker they select their mates and begin mycelium migration across the plate. So you place your drop dead center of the dish. One...

Yes, one drop is plenty

Agar dishes and then sector selection then another dish then liquid culture then test.

Yeah, suppress inoculations are pretty hit or miss

There have to be other ways dude. What sort of experience are you looking for?

Well, for me anyway, I run three reactive hepa systems and an electrostatic. Tests show that the spore load in the room is about the same whether I use them or not. It's lower in a sphere of about two feet from each but not a whole lot. The room is not hermetically sealed.

And of course the actual name. No turbulence, if you have a near perfect hole in a container of water (or emanating from a pump) the water will flow out as a perfect cylinder of water all the way to the end. That is laminar flow. Any rough edge in the hole will introduce that turbulence. Now...

Agreed.

Is that laminar? Remember that it is the smooth flow of air that keeps particulates out of your work. Turbulence will invite contamination on plates and such.

Then that's a pretty quick growth huh.

Your corn is still a little undercooked tho. I find that it works best when the kernels have just slightly burst but then again, if you are using it as spawn it should work fine. That's pretty quick. What temp?

Yeah, no doubt at all

I just watched it again, it's not quite what I described but what is to keep one from simply attaching a needle to the end of the tube and just using that as your inoculation syringe rather than filling individual syringes? Replace the needle after so many innculations.

I like just using a drop or two of food coloring. Very handy for organization and the more whispy mycelium.

Well they make a sort of self filling, "dosing" syringe setup ( was that ctually what i saw in the vid? Duno) that I have had my eye on for along time i think they are repeated pipettors but 60 ml syringes will allow 12 bags to be adequately innoculated. There are what? 150's? 160's out...

I use dextrose, karo and various steeps depending on the intended spawn material. Corn steep, straw steep, coir steep or manure steep. If I am concerned about sencience I will change them up. I am still uncertain of the advantage of ketones and yeast extract but I use It anyway. If I am...

Don't use hot needles, firing your needles really serves no purpose. Firing scalpels does because you are definitely making contact with mycelium or spores. Needles are cheap and you can successfully autoclave them over and over again. We mycological folk learn a super power, you have it I'm...

How big are your syringes?

I am not Thanks, I am not much worried about that conglomeration effect as I am not growing that particular species. I have noticed that constant stirring serves no purpose but when I have done so it was too slow to have dissolved much oxygen. When I do spin it, I do so to the point of...