would there be a difference?

is the potency and quality lower in a 3rd generation mother than a 1st generation mother plant?

by saying 3rd generation i mean taking a clone cutting and letting it grow then taking another cutting from the one you just grew then letting that one grow then taking a final cutting

I wouldnt think so but if it was it would be so little of a difference it wouldnt matter.

nope....the plant doesn't change...it is the same plant

When I started I found a seed in some really good dank and I have been growing for about a year with the same clones, mother troubles. It seems to get better every time I harvest.