Tissue culture and cannabis?

coconutbeach said:

Check out Microclone Cannabis Tissue Culture. Search Google.

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Sure will. Thanks for the tip. I plan on incorporating this method on my next move to a place with more space. I know it'll take a while to get the hang of it, but sometimes I just want to put a clone on the back burner for a while, but keep it, but keeping a mama plant I don't plan on flowering for a while really takes up time and space. Tissue culture ftw

mudballs said:

i reviewed that and all that paper describes is the most expensive method to root clones.
native humbolt has the most pertinent info to proceed. sterilization and proper vitamins mix.
i'm gonna send you to this thread
https://www.rollitup.org/t/scarholes-root-pruning-sex-reversal-method-for-fem-seeds.853829/
in this thread scarhole discusses a prominent breeder culturing root cuttings. if you go down that rabbit hole you might find a way of just taking a cutting from a root and growing a plant.imagine how many more clones you could get from a mother? i'm fascinated by it but not in a position to pursue it at the moment.

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Your wish is granted.

MikeGanja said:

Mudballs, I agree. It looks like the process probably could be made at home to a somewhat reasonable cost but the kits like the one in the link doesn't guarantee a successful clone with cannabis. I would be more convinced if Bill Graham show us examples with cannabis instead of redwood.

Native Humboldt, great news that I could get the needed equipment for $ 100. It's less then half the price I found so far.. Sorry to hear that you didn't succeed in finding a working formula for cannabis.

Some Asian scientists have published a study on tissue cloning of cannabis. Do you think it can be useful for growers like us? --> http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf

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Hi Native. It would be my pleasure. The instructions are the same and we will be producing a cannabis tissue culture kit as soon as we get the legal "go". Here are some TC cannabis pics of the kits in action and a tip sheet for making starts in liquid medium. The media in the Microclone kit are excellent for cannabis as well as enough other plants and is still the most popular cannabis TC media made. Thank you and good luck.

coconutbeach said:

Regular clone ROOTING is much faster because of the resources put into creating the 6" + cuttings.

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Yes. Which makes TC attractive if putting resources into those cuttings would cost so much in resources (space and light for mother plants) that it justifies TC, which only makes sense when you need shit loads of clones, but then, if you need those for yourself it means you veg and flower a shitload of them too, which gives you plenty of branches (need only 1 per plant in theory, a few for backup) to use as cutting, especially when the plants are subject to some canopy control such as topping.

coconutbeach said:

Hundreds of clones are produced from only 8 sf under 100 watts of light every month.

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Hundreds of plantlets. The required space and light for any amount of "clones" depends on the size of those clones and not on whether they are from tc plantlets or regular cuttings.

I totally get the appeal and TC has its useful purposes but in practice TC requires additional space and resources, besides time.

The increase in numbers is from the ability to make many from little, as in many plants from a single leaf or a calyx for example. It's not about being able to produce the same number of clones in less space. In practice it's particularly useful for companies who sell millions of clones to thousands of growers.

Growing a cutting in sugar an then calling it tissue culture, ending up with a weak clone, is almost as silly as some other guys at another forum pretending to do tissue culture with seeds in agar...

^^^^

You're right, TC does not yield a superior cutting, if you observe the premium cuts offered by Dark Heart Nursery vs the TC clones, they have thinner stalks vs the conventional cuttings. They are using TC for the high volumes needed for a commercial cloning operation.

If cannabis was an unregulated plant, it would make more sense to simply take clones in a conventional manner. Since no specialized equipment is needed. TC is more or less for plants that are hard to duplicate, as observed in the agri industry.

Cannabis hybrids could still benefit from TC:

-Pathogen free stock..
-Duplication hard to clone cannabis strains.. like Ruderalis hybrids.

The seed to agar method could explain that particular method of TC.

Another thing TC could be used for is dealing with "duds" or old cuttings, or virus infected stock. The theory is that newest growth is less likely to be infected with pathogens, TC could be use to create clean clones. And people seem to be talking about TC giving vigor to dudded out cuttings.

You had me at "you're right" already and then you put "duds" between quotes too... but then you mentioned cloning ruderalis... Ah well good post anyway.

And yes, rejuvenation of cuttings stripping the results of (mis)nurture from nature could be one of the more useful purposes of tc for a tiny amount of growers. Not enough to make it common practice for cannabis growers though.

Many of the large commercial cannabis grow ops today are just inflated homegrows. Once pros take over the industry TC for cannabis may become more common but I don't think it will ever become common for home and other small growers. Those TC@home kits are a bit silly unless it's for educational purposes. Practical it is not.

Sativied said:

You had me at "you're right" already and then you put "duds" between quotes too... but then you mentioned cloning ruderalis... Ah well good post anyway.

And yes, rejuvenation of cuttings stripping the results of (mis)nurture from nature could be one of the more useful purposes of tc for a tiny amount of growers. Not enough to make it common practice for cannabis growers though.

Many of the large commercial cannabis grow ops today are just inflated homegrows. Once pros take over the industry TC for cannabis may become more common but I don't think it will ever become common for home and other small growers. Those TC@home kits are a bit silly unless it's for educational purposes. Practical it is not.

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I'm not knocking on the homegrower, because that includes myself. But its nice to to have that tool if needed.. I would rather mix my own agar for educational purposes, and to see if its something that could be used on a commercial scale.

Anybody should be able to observe in industry that some operations are specialized.. same applies to agriculture, TC is one of those, thats pretty much all they do, if a farmer/gardner needs some plants they simply go to the nursery. Seems like misuse of resources for a warehouse grow to propagate cuttings with the TC method when they should be using that for flowering.

Just a couple of notes I'd like to share about tissue culture when working with hormones.

1) The ammounts in TC are very small, you'll have to be careful with your math and you'll need a accurate scale to weigh the small ammounts.

2) IBA does not dissolve in water.. It will dissolve in 100 proof ethanol (I like smirinoff). Then you can dilute it with water to mix into your agar.

3) Kinetin does not dissolve in water, you will need to mix a caustic solution to dissolve it. 1 teaspoon of Sodium Hydroxide (Lye) will work to 1/4 cup of water, dissolve the lye first, then add the kinetin. Then dilute with water. General Hydroponics PH UP will not work.

Be very careful with any type of strong caustic, when pouring the lye grains, when I poured it into a dish, some of them bounced out of the shallow pyrex dish, lye isn't active until you touch it or it gets wet then it will burn the crap out of you.

When you guys figure it out let me know?? Its easy but it tskes to long in my opionion..

too long for me homie, i ditched that shit lol ill just keep cuts in the fridge if i need to...

thump easy said:

When you guys figure it out let me know?? Its easy but it tskes to long in my opionion..

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I got it figgured out, if you ever get around to posting your formula, i'm all ears.

Ill try but it might be a while