Rubisco456
Member
Straight up: What is your opinion on the importance and the role that callus formation plays in the cloning process?
Do you use callus formation as an indicator of successful clones?
Do you care about callus formation?
Do you induce its formation?
I’ve been doing a lot of research on plant anatomy and cell culture for a grow that I’ll be working with and in my time working with the grower I’ve heard them say that the callus formation is the signal that they wait for to make the call that the clone is going to make it. I really like that idea because the callus will seal off the wound and can give rise to roots but I’m curious to know what the general community thinks about this technique or if it’s a widespread practice?
For those that are unfamiliar with the term Callus - callus, In botany, soft tissue that forms over a wounded or cut plant surface, leading to healing. A callus arises from cells of the cambium. When a callus forms, some of its cells may organize into growing points, some of which in turn give rise to roots while others produce stems and leaves. Thus a callus may be capable of regenerating an entire plant.
Here is a picture of callus in plant cell culture:
Plant cells demonstrate the ability to be totipotent that is: a single cell in a plant can give rise to a whole other plant if given the correct hormones - unlike animals cells that are LOCKED into their roles (I know people are messing around with inducing some mammalian cells to be pluripotent) this is how plant cell culture is possible.
Do you use callus formation as an indicator of successful clones?
Do you care about callus formation?
Do you induce its formation?
I’ve been doing a lot of research on plant anatomy and cell culture for a grow that I’ll be working with and in my time working with the grower I’ve heard them say that the callus formation is the signal that they wait for to make the call that the clone is going to make it. I really like that idea because the callus will seal off the wound and can give rise to roots but I’m curious to know what the general community thinks about this technique or if it’s a widespread practice?
For those that are unfamiliar with the term Callus - callus, In botany, soft tissue that forms over a wounded or cut plant surface, leading to healing. A callus arises from cells of the cambium. When a callus forms, some of its cells may organize into growing points, some of which in turn give rise to roots while others produce stems and leaves. Thus a callus may be capable of regenerating an entire plant.
Here is a picture of callus in plant cell culture:
Plant cells demonstrate the ability to be totipotent that is: a single cell in a plant can give rise to a whole other plant if given the correct hormones - unlike animals cells that are LOCKED into their roles (I know people are messing around with inducing some mammalian cells to be pluripotent) this is how plant cell culture is possible.
