Hey Guys -
Heres a explanation from Papa Powwow from advanced nutrients:
The Basics:
Propagation of Marijuana starts with producing and germinating of seeds. Once you are working with established plants, your job is much easier. One of the keys to producing viable seeds is the proper collection, and if needed, storage of pollen. I try to make sure the pollen I use is as viable as possable. I am going to discuss how to go through the whole process. Pollen can stay viable for at least a week or two at room temperature. I don't recommend waiting this long because it is important that pollen be as fresh as possible. Using pollen that is older is still better than nothing, if that is all you have to work with. Cannabis plants are a dioecious species. (possessing separate male and female plants). Male plants produce pollen and females produce Flowers(buds) and seeds. Sometimes a hermaphrodite (Bisexual) plant with both male and female flowers will occur.Male plants are typically thinner and taller than female plants.
When a female flower is pollinated, one of the many, grains of pollen from the male (staminate) flower pod, comes into contact with a pistil on the female (pistillate ) flower. Female flower tops are a mass of calyxes with each calyx containing a ovule and a set of pistils ( the little white hairs). Once fertilization takes place The pistils turn orange or brown and a seed starts to grow within the seed bract! Just like people, seeds will contain genetic characteristics of both parents. But thats another story!
Getting Down To Bizz:
When collecting pollen from a male you will notice that some of the pollen sacs are ruptured, when you see that pollen has fallen out, put a piece of paper under the pods to catch any pollen and tap the male pod slightly with your finger to see if the pollen falls out . I don't recommend newspaper because it is coarse and the pollen sticks to the paper, and never use plastic baggies!! Notebook paper works very well. If ANY pollen falls from the pods, it is ready to harvest. Store the pollen in paper packets made by folding small pieces of notebook paper and sealing the side and ends with tape. It is important to make sure that the tape covers as little of the surface as possible so that the paper packet can breathe.
In order to store pollen for long periods of time, it is important to remove as much of the water content in the pollen as possible. Store the pollen in a glass jar or vial, avoiding plastic bags, which can breathe and are not the best for long-term storage. To reduce the water content in the pollen, place some desiccant in the bottom of the container. Its best to use an indicating desiccant. For example
.. When dry, it is blue; as it absorbs moisture, it turns pink. By using an indicating desiccant, you can tell when it has absorbed all the moisture it can. (It is impossible to tell whether regular desiccant is still dry just by looking at it)
Once indicating desiccant has absorbed all the moisture possible, it turns pink. Place it in the oven and bake it until it turns blue again. This desiccant can be used over and over again.
The amount of desiccant you use depends on the amount of pollen you are trying to dry out. I have found that 1/2 - 1" in the bottom of the container works well. The paper packets are then placed on top of the desiccant. By using paper packets, which can breathe, the desiccant can do its job. Instead of using one big packet for all of your pollen, place the pollen in small packets so one packet can be removed quickly without disturbing the unused pollen. Try to put enough pollen in each packet to pollinate a single bud once. If you plan to pollinate the same bud a few times, fresh pollen can be removed each time for maximum viability. Just use a small painters brush to gently apply the pollen to the pistils. Write the strain name and date stored on both sides of the packet so you can tell later on how old the pollen is. You should also tag the branch with the flowers you pollinated. You may want to pollinate a few differant bud's on the same plant with different strains of pollen. This can become very confusing if you don't use tags, Wright the name of the cross and the date you pollinated for future references.
Once you cap the vial, let it sit in a dark ,cool, dry place for 24hrs. Then put it in the refrigerator for three days. Do not place it directly in the freezer because the pollen's moisture content is too high; water expansion can rupture the pollen and render it inviable. Once the pollen has been in the vial for at least two days, and as long as the paper packet has had enough breathable area, moisture content will be reduced enough for you to put the vial in the freezer. Once the vial is in the freezer, the pollen should be good for years. Pollen will stay very fresh for at least three years. If you collect pollen on separate occasions, but eventually want to keep the pollen in the same container, it is best to store the newer pollen in a different vial and go through the same process in the refrigerator. Once the new pollen is desiccated, the newer packets can be quickly placed in the original container. When removing packets for use, it is important to minimize the time that the vial is open because the pollen and packets can reabsorb moisture. This is another reason to use several packets instead of removing a small amount of pollen each time from one larger packet. Once you remove a packet, use the pollen as soon as possible.
Pollen loses viability quickly at room temperature and even faster at higher temperatures. Pollen can stay viable at room temperature for several days, but I attempt to maximize its viability so that more good seed is produced in the long run. Just because an entire bud is pollinated does not mean all of the seeds will germinate. Quality of the pollen has a great deal to do with how many seeds in each bud are viable. Another reason to keep moisture content low in pollen is to lessen the chance for fungus to grow on and kill the pollen. When sending pollen to someone through the mail send it in a vial containing desiccant. This will keep the viability high. If the shipping time will be longer than one week, Add a cold pack to the box, If dry ice were used, pollen most likely could be shipped anywhere in the world without loss of viability.
I hope this information helps everyone store pollen correctly to insure its viability. This is especially important for those who are mailing pollen to others. More and more people are propagating growing and breeding their own marijuana every day, and they are also coordinating their efforts with others by sending pollen to people who do not have the room for male plants, this will help everyone produce more seeds more hybrids/crosses and, eventually, overgrow the damn government!