EverythingsHazy
Well-Known Member
Due to the fact that you can't backcross to a female auto plant (for this thread we will use DP Think Different "TD") because it will die beofre the next generation is ready, and you cant get pollen from it to store, how well would this work?
Female TD x LR2 male
=F1
NEW Female TD x F1 male
=F1 (second batch)
NEW Female TD x F1 (second batch) male
= F1 (third batch)
F1 (Third Batch female) x F1 (third batch male)
F2, etc
How much would the new babies in the final F1-F7(stabilized) generations be genetically similar to a pure TD?
Had it been backcrossing, it would be first (before backcross) 50/50, then 75/25, then 87.5/12.5/ then 93.75/6.25. It should be almost identical to the TD at that point. BUT:
1. You arne't backcrossing, just crossing back to a new TD this method.
and
2. How many times can you backcross to keep bringing the perecnt of Td higher? In theory it should be infinite getting closer and closer to 100% but never completely making it there. So using 7 fem seeds, you should be able to
Also, if you were to put an autofem seed into sterile culture (flasking/tissue culture) and then put it on media with high callus forming hormone concentrations to prevent it from forming any maturing shoots or roots which would most liekly being the autoflower ocuntdown lifecycle, would you be able to keep moving bits of callus onto rooting media to create new clones of the mother to get a legitimate backcross? That wya you would be able to turn an autofem TD and a Lowryder 2 male into a strain that is virtually identical to the original TD and producing stable seeds of male and female within a couple years. THat is just a theory of mine. It might turn out that the callus and multiplying tiny dividing plants in tc would jsut start flowering at a ridiculously small size and the entire batch would be gone in a couple months. Anyone have any knowledge on this?
Female TD x LR2 male
=F1
NEW Female TD x F1 male
=F1 (second batch)
NEW Female TD x F1 (second batch) male
= F1 (third batch)
F1 (Third Batch female) x F1 (third batch male)
F2, etc
How much would the new babies in the final F1-F7(stabilized) generations be genetically similar to a pure TD?
Had it been backcrossing, it would be first (before backcross) 50/50, then 75/25, then 87.5/12.5/ then 93.75/6.25. It should be almost identical to the TD at that point. BUT:
1. You arne't backcrossing, just crossing back to a new TD this method.
and
2. How many times can you backcross to keep bringing the perecnt of Td higher? In theory it should be infinite getting closer and closer to 100% but never completely making it there. So using 7 fem seeds, you should be able to
Also, if you were to put an autofem seed into sterile culture (flasking/tissue culture) and then put it on media with high callus forming hormone concentrations to prevent it from forming any maturing shoots or roots which would most liekly being the autoflower ocuntdown lifecycle, would you be able to keep moving bits of callus onto rooting media to create new clones of the mother to get a legitimate backcross? That wya you would be able to turn an autofem TD and a Lowryder 2 male into a strain that is virtually identical to the original TD and producing stable seeds of male and female within a couple years. THat is just a theory of mine. It might turn out that the callus and multiplying tiny dividing plants in tc would jsut start flowering at a ridiculously small size and the entire batch would be gone in a couple months. Anyone have any knowledge on this?
