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I've grown out Nigerian Sunshine and Blue Orca Haze and never saw any intersex traits on males or females.

To each their own, brother. Some people throw out the baby with the bath water, some don't. I don't have a vested interest in any other then my businesses and sharing knowledge. Yes, I teach tissue culture classes in Northern California and perform tissue culture services. I'd much rather...

When you excise the meristematic dome you're working with a cluster of undifferentiated cells about 0.25mm in size. It's maybe a few hundred cells at most. Cannabis is totipotent which means, theoretically, a single cell can grow into an identical replica of the plant it was sourced from...

First, I want to make sure that I'm clear by stating that I'm not a DHN fan boy. Have I used their diagnostic services? Sure. Did I see there clones all over the SF Bay for years? Sure. Did they help populate hplvd? It appears that way but they were also one of the only major nurseries bere...

In person. Sacramento, CA. I'm recording other online content but, in my opinion, you're cheating yourself if you are not taking advantage of doing hands-on training.

This is a pretty silly statement. Sorry, I cannot tell if you are saying inlt tongue-in-cheek. Hplvd has been decimating the hop industry for a long time. Yes, Dark Heart passed it all over California with their clones but also wrote the white paper on its presence in cannabis. I admit there...

I'd be careful about making this statement without clarifying the differences between nodal tissue and meristematic tissue. Nodal culture is merely aseptic stem propogation. Meristematic tissue is not technically connected to the plants vascular system and culturing it can reset the...

I use plates for callus, otherwise, tubes, tubs and boxes

I don't pop in that often but look what I stumbled across. Hplvd (and other diseases) can be eradicated by dissecting and culturing the meristematic dome. (~0.25mm) You're agar/MS microprop kit may work if you use a microscope, clean blades with every cut, and have really good sterile...

I'd be very skeptical if ANYONE offers to clean a plant of hplvd for $500. It requires culturing the meristematic dome tissue (~.25mm) and can take up to 10-12 months to get a full size rooted plant. I teach people how to do it but most of us charge $5000 to do this kind of service. It...

It sure looks like ph to me too but happy frog is also pretty hot for babies. Are the other in happy frog too? Maybe tip it upside down and knock it out into you hand to see if the bottom soil is soggy? If not I'd say too hot or too wet.

Sure thing, I'm here and there and everywhere. You're in for a treat with that Nigerian sunshine x Goji cross. Expect purple!

My HLgs were DIY's and I now have a larger space that I am automating. I have a 5x5 lab tent with two 480w 4-board panels. Now I have a 10x25 seqled room with 4 gavitas at one end and space for another 4, easily

I just moved on from my HLG 4 board panels and bought a bunch of gavita 1700e's for less than $1000 each. (Automation, etc) Keep in mind that most lights will only cover a 4x4 area well and a 5x5 area OK. Phresh makes great filters but don't discount AC Infinity. They are cheaper and work well.

It looks like mites but there should be webs if damage is that bad. If there are no bugs I'd consider checking the ph and ec of what goes in vs what comes out.

I would not use a petri dish unless your goal is undifferentiated cells. Use a deli tub or test tube that you can autoclave

I've been doing TC work since 2017 and am teaching classes now in the lab that I built out. Don't buy a kit. Just roll your own media mixes. If you have mastered the aseptic technique required for myco work then TC is easy. You'll have a steep curve learning protocols but you could begin...

I bought four Gavita 1700e's but still have my two 4-board qb288s rocking in the nursery. Each pulls 480w at the wall and I had all 8 boards dimmed in a 5x5 lab tent for a while. They are super efficient dimmed down to about 75%

Sterile technique itself is part or how people cultivate mushrooms. Since it's a technique (creating a completely sterile workspace) it can be done as simply and inexpensively as sitting a plastic tote on its side and working inside it, and as complex and expensive as having a full scale clean...

Pull off the lowers and let it ride. Hermie nanners typically express on the lower branches but one open cluster creates a seeded shit show. It's really a personal preference. Do you like to smoke seeds? I don't. Do you like to go away for the weekend? I do. Plants like this will need...