Tissue Culture Opinions & Experiences

K

Kervork

Well-Known Member
I have mostly mastered culturing fungus and can do agar to agar transfers, agar to grain etc. all day long in a still air box with a high rate of success. The videos I watched on tissue culture looked even easier. I have a whole bunch of high end seeds. I'm curious about what experience others may have had doing this on a small scale.

There are legal limits on the number of plants I can have. Unfortunately as near as I can tell plant is not defined by the law. I would like to think the state has better things to do than argue with me about green slime in a test tube, but who knows. I would like to be able to maintain an extensive genetic library. Maybe even make chimeras or something wierd.

I considered just piecing everything together, a lot of the kits were kind of gay. I did find this one https://phytotechlab.com/hemp-multiplication-kit.html which seems to be not totally gay. I figured it would be easier to get a kit initially and then build out from there so I didn't have to learn everything at once. I do intend on figuring it all out and eventually just buying raw materials.

Basically I'm curious as to what to expect, what worked, what disappointed you, what would you have done differently. Yeah, I can make clones all day long but I'm autistic and like doing things the hard way. Growing weed in a test tube seems like the moronic type of thing I would enjoy.

Now that weed is legal I'm taking a longer term view. Glutting the market ain't much of a business plan so I'm focusing on fiddly technology and genetics.
 
vostok

vostok

Well-Known Member
a smart-gay-autistic- moronic-culturist may have gone so far as do a search here on RIU on these often repeated request HERE

but in truth the plants take too long to grow, the conditions too tight and very little results are spent forth, time is spent in better areas...like growing!

few here would boast that they can grow Fungi/Mold

good luck
 
hotrodharley

hotrodharley

Well-Known Member
The systems used in legal states track it seed-to-weed. There’s no cost per plant. I’m talking legal recreational operations that some exceed 10K square feet of lighted canopy. Each plant is assigned a METRC number and is entered into the tracking system. You might check how Michigan addressed this. There’s a place 7 Engines that states their doing, or planning to do, tissue culture.

Here in Alaska I don’t know of a single operation using it. Like @vostok stated.
 
xtsho

xtsho

Well-Known Member
I think I looked at that exact same kit awhile back when I was contemplating tissue culture. I decided I didn't want to bother with it so I just make seeds the old fashioned way.
 
vostok

vostok

Well-Known Member
Guys don't get me wrong here... their is space for this in our hobby, but finding time -
time when the 'normal' grower on his 3rd-9th grow would be better spent
understanding femminization collodial siler even sts or basic cloning

but we could say the same thing over home brew and not having a still...?
 
K

Kervork

Well-Known Member
vostok said:
Guys don't get me wrong here... their is space for this in our hobby, but finding time -
time when the 'normal' grower on his 3rd-9th grow would be better spent
understanding femminization collodial siler even sts or basic cloning

but we could say the same thing over home brew and not having a still...?
Click to expand...
vostok said:
a smart-gay-autistic- moronic-culturist may have gone so far as do a search here on RIU on these often repeated request HERE

but in truth the plants take too long to grow, the conditions too tight and very little results are spent forth, time is spent in better areas...like growing!

few here would boast that they can grow Fungi/Mold

good luck
Click to expand...
Countless request but very little from people actually using it for production or at scale that can give a qualified opinion.
If you don't try, then you don't learn and if you don't learn you don't improve. I was told all sorts of things about fungi, turns out most of them were totally wrong. There is a reason most people here seem to grow Trichoderma instead of mushrooms.

Doesn't make much sense to view a screwdriver in the context of what a hammer does. The question is, as a tool what is tissue culture well suited for and what are it's limitations and what preconceived notions are likely to be wrong.

Looks to me like it gives far more exacting control over genetics on a very large scale than cloning via cuttings does. That's something that may be useful to me. Since I already have all the equipment set up and need only chemicals would be kind of stupid not to try it and at least see what it can do.

oyster.jpg
 
jimihendrix1

jimihendrix1

Well-Known Member
The thing to watch out for is getting Bacteria/Virus in the Agar with the tissue sample.
I did the process from a KIT I got from Worms Way back in the 90s.
Did it in a bathroom with no windows, no curtains, no carpet, one constantly sprayed things down with Lysol. You made a box out of an aquarium, plexiglass, and had to sterilize all that. Have to make sure no dust is floating around in the air. Dust gets in, its over.

One cannot expose the tissue culture to any kind of UV. EVEN 1 Short flash, and theyre dead. Cool White Fluro is what was used then.
All instruments have to be constantly sterilized, and make sure nothing is floating in the air, and the box you make is to the specs they tell you. I cant remember now exactly how to make the box. One used Gloves. You have to sterilize the tissue.

But the jist of the process is the box needs to be all but airtight, everything must be beyond sterile, nothing can be floating around in the air, and one has to constantly spray shit down, and NO UVA/B at this point.
Any kind of bacteria, fungus, virus ect that is present with the tissue, will kill the tissue, the other substances being less complex, will propagate at the expense of the tissue culture.

I believe the tissue sample size I used per test tube was 1 sq/cm, and was placed upright, not flat.

Its a long process, and on has to transplant the tissue a couple times, at least one did, I have no idea how they may have it done now..... its also a bitch to separate the "Plantlets" Then one has to harden them off to UVA/B

Thats all I got for now.

Tissue Culture also takes the plant back to its beginning vs simply cloning it. Its just a very long, tedious process, that IMHO can be very rewarding.

I never got to fully explore it, as my partner in crime got caught with 500 clones I gave him, and he ratted me out, and they got 500 more, and my tissue cultures. I only had maybe 50 plantlets. 4 test tubes is all..... I was still learning. I was doing clone like a mofo though. I then spent from 97-2009 with the feds.
 
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K

Kervork

Well-Known Member
jimihendrix1 said:
The thing to watch out for is getting Bacteria/Virus in the Agar with the tissue sample.
I did the process from a KIT I got from Worms Way back in the 90s.
Did it in a bathroom with no windows, no curtains, no carpet, one constantly sprayed things down with Lysol. You made a box out of an aquarium, plexiglass, and had to sterilize all that. Have to make sure no dust is floating around in the air. Dust gets in, its over.

One cannot expose the tissue culture to any kind of UV. EVEN 1 Short flash, and theyre dead. Cool White Fluro is what was used then.
All instruments have to be constantly sterilized, and make sure nothing is floating in the air, and the box you make is to the specs they tell you. I cant remember now exactly how to make the box. One used Gloves. You have to sterilize the tissue.

But the jist of the process is the box needs to be all but airtight, everything must be beyond sterile, nothing can be floating around in the air, and one has to constantly spray shit down, and NO UVA/B at this point.
Any kind of bacteria, fungus, virus ect that is present with the tissue, will kill the tissue, the other substances being less complex, will propagate at the expense of the tissue culture.

I believe the tissue sample size I used per test tube was 1 sq/cm, and was placed upright, not flat.

Its a long process, and on has to transplant the tissue a couple times, at least one did, I have no idea how they may have it done now..... its also a bitch to separate the "Plantlets" Then one has to harden them off to UVA/B

Thats all I got for now.

Tissue Culture also takes the plant back to its beginning vs simply cloning it. Its just a very long, tedious process, that IMHO can be very rewarding.

I never got to fully explore it, as my partner in crime got caught with 500 clones I gave him, and he ratted me out, and they got 500 more, and my tissue cultures. I only had maybe 50 plantlets. 4 test tubes is all..... I was still learning. I was doing clone like a mofo though. I then spent from 97-2009 with the feds.
Click to expand...
Now this is useful. The sterility I knew about and won't be an issue. I have a glove box and can play with agar without it turning green. I had no idea about issues with UV though. Many have asked about autoflowers and tissue culture and whether it resets the clock. I haven't found anything yet which fully answers that.

There is a big difference between the needs for high THC clones and high CBD clones. With CBD clones there is no margin for error. You need thousands of plants that are genetically identical, not just close. If you were dealing with many thousands, tens of thousands of clones would tissue culture win out?
 
SCJedi

SCJedi

Well-Known Member
I've been doing TC work since 2017 and am teaching classes now in the lab that I built out.

Don't buy a kit. Just roll your own media mixes. If you have mastered the aseptic technique required for myco work then TC is easy. You'll have a steep curve learning protocols but you could begin with some papers and some TDZ or m-topolin. I would use PPM at first to reduce the level of contamination.
 
Milky Weed

Milky Weed

Well-Known Member
I will have to look into this, I also have abit of experience with agar work ;)

I had no idea you could grow plants on a Petri dish… I thought they needed roots!
 
SCJedi

SCJedi

Well-Known Member
Milky Weed said:
I will have to look into this, I also have abit of experience with agar work ;)

I had no idea you could grow plants on a Petri dish… I thought they needed roots!
Click to expand...
I would not use a petri dish unless your goal is undifferentiated cells. Use a deli tub or test tube that you can autoclave
 
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