Freezing pollen?

So I’ve got some feminized pollen from gelat OG, currently drying it in Tupperware with some silica gel. I’ve placed an RH metre inside and I’m hoping to get it down to about 20-30% RH. Will this be dry enough to preserve viable pollen when I freeze it? This is my first time trying to freeze some for my next crop.
 

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CannaOnerStar

Well-Known Member
When you take it out, dont open it immediately. It being colder than room temperature, it will start to condensate water on it and pollen does not like water at all.

Rice is good to put in the vial as mentioned, but you can also mix some maizena(corn flour) or other similar flours with the pollen to take some of the humidity away. Its mostly useful if you have very little pollen. Also if you have more, you might want to store it in separate vials, so that you dont have to move everything between freezer temp and room temps. Pollen does not like that either, even if you would be careful with the condensation(which is the major potential issue).

I like to keep the pollen vials in a small minigrip that has silica in it and that smaller minigrip in a larger minigrip.
 

xtsho

Well-Known Member
I actually started to mix mine with a little cornstarch. I then put it small plastic tubes that have airtight lids. I also put some of the silica beads in with it as well. Then I vacuum pack those and they go in the freezer.




Also don't add flower

It thins it out wayyyyyyyyyyyyyyyyyy to much and viability is damaged. Had many none pollinate with it, and same stuff in rice was perfect

Raw rice for the win
A lot of people mix with flour. But you need to make sure it's dry. Flour will absorb moisture from the air so it needs to be dried out in a warm oven prior to mixing.

But if you're having success with rice then keep doing what you're doing. If it aint broke...
 

CannaOnerStar

Well-Known Member
Not a fan of flour at all

Have u used your flour pollen yet?
I have mixed some corn starch every time except once, never had any issues. Corn starch is almost the same consistency as pollen is and it sucks the moisture form air pretty much as easily as pollen does. Rice works well, but its slow acting like silica, it does not protect from exposure to air if you open the vial etc. it only helps to suck the moisture from inside the vial and does it much slower than corn starch, simply because it has less surface area to it. Rice is a long term protection, corn starch protects from sudden moisture damage and long term.

I put a few grains of rice and mix some corn starch just o be sure it doesent get any moisture damage. Im sure im overdoing it, but better safe than sorry..
 

xtsho

Well-Known Member
Not a fan of flour at all

Have u used your flour pollen yet?
I haven't used any from the freezer yet but I did pollinate plants prior to freezing after it was mixed with the cornstarch. I've got the seeds to show for it. But I'm sure the frozen pollen will work when I go to use it as it was dry, sealed, and then sealed again with desiccant packs.
 

BobThe420Builder

Well-Known Member
I just used, and have before, 18mo old pollen, in rice, opened up..diped out, multiple times, and always pollinated


If you have no actually experience, thinking one is better than the other is false claims

I've done them both....

Just saying
 

xtsho

Well-Known Member
I just used, and have before, 18mo old pollen, in rice, opened up..diped out, multiple times, and always pollinated


If you have no actually experience, thinking one is better than the other is false claims

I've done them both....

Just saying
Nobody said one was better than the other so I don't know what false claims you mean.

There are breeders that mix their pollen with flour for long term storage. It's a common practice that's been used for years with success. Rice works as well. I don't know why your experience didn't turn out successfully but there are many people pollinating their plants with pollen stored that way.

In fact they've now frozen pollen mixed with flour and then froze it in liquid nitrogen and have been successfully pollinating females with the sample. That means that you could store pollen indefinitely if you had a cryostorage tank. But those are expensive.


"To preserve the pollen, the researchers removed any moisture, added the result to baked whole wheat flour and preserving agents and then froze it in liquid nitrogen. When they removed the mixture from the liquid nitrogen and applied it to flowering female plants, it resulted in successful seed formation in all of the subjects."

“Finally, we have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen,” it concludes.

 

BobThe420Builder

Well-Known Member
K.I.S.S.
I just press a nice pinch of pollen onto cellophane, remove any other vegetable matter and press it so no air sticks around. Then I just Tape it up with masking tape, label it, and into the freezer it goes. No flour, no rice. And months later, it still pollinates sucessfully.
Interesting
 

xtsho

Well-Known Member
1st hand experience here

Not Interwebs bs
Interweb BS?

Could you even be bothered to read the article? It's not BS. It's interesting stuff. You're response is just bizarre.

The guy using baked pollen has a PhD., is Vice President of research at the University of Lethbridge, has 150 peer reviewed publications and 10 patents. He's involved in studies that they spend millions conducting. He's using baked flour so I don't know how you can call that interweb BS.

Like I asked. Did you bake the flour before you used it? Most flour is 10 - 14% moisture. So if you didn't bake it you stored your pollen with flour that contained moisture. Using flour is not BS. Flour is used to extend pollen for other plants as well. It's use is all over the place from Universities to just about every cannabis site or forum that has a topic on storing pollen.

I'm going take guidance from someone with a background in plant biotechnolgy and a plethora of other advanced topics than some dude on the interweb as you call it. The guy is doing research on the long term storage of cannabis pollen. Not just 18 months but theoretically indefinitely.

You're free to use whatever method you wish. But to go on about people making false claims and interweb BS is ridiculous. I've provided data based on a scientifically conducted study by people much smarter than you or me with regards to this subject.


Dr. Kovalchuk’s research studies genetic and epigenetic regulation of plant responses to stress, while also developing methods for improving plant transformation. As a researcher in epigenetics, epigenomics, bioinformatics, genetic engineering, plant biotechnology, and next generation sequencing, Dr. Kovalchuk has over 150 peer reviewed publications and holds ten patents.

 

BobThe420Builder

Well-Known Member
Interweb BS?

Could you even be bothered to read the article? It's not BS. It's interesting stuff. You're response is just bizarre.

The guy using baked pollen has a PhD., is Vice President of research at the University of Lethbridge, has 150 peer reviewed publications and 10 patents. He's involved in studies that they spend millions conducting. He's using baked flour so I don't know how you can call that interweb BS.

Like I asked. Did you bake the flour before you used it? Most flour is 10 - 14% moisture. So if you didn't bake it you stored your pollen with flour that contained moisture. Using flour is not BS. Flour is used to extend pollen for other plants as well. It's use is all over the place from Universities to just about every cannabis site or forum that has a topic on storing pollen.

I'm going take guidance from someone with a background in plant biotechnolgy and a plethora of other advanced topics than some dude on the interweb as you call it. The guy is doing research on the long term storage of cannabis pollen. Not just 18 months but theoretically indefinitely.

You're free to use whatever method you wish. But to go on about people making false claims and interweb BS is ridiculous. I've provided data based on a scientifically conducted study by people much smarter than you or me with regards to this subject.


Dr. Kovalchuk’s research studies genetic and epigenetic regulation of plant responses to stress, while also developing methods for improving plant transformation. As a researcher in epigenetics, epigenomics, bioinformatics, genetic engineering, plant biotechnology, and next generation sequencing, Dr. Kovalchuk has over 150 peer reviewed publications and holds ten patents.

Ok



And I have real life experience
 

xtsho

Well-Known Member
Ok



And I have real life experience
Same thing with people that have used BAKED pollen. Nobody said rice would not work. But because you didn't follow the process for using flour correctly and were unsuccessful using it you say people are spreading false information. It's not false. Saying flour doesn't work is spreading false information.

Like I said. You need to dry out the flour. If you didn't then you mixed moisture containing flour with your pollen. Flour can contain a lot of moisture. I used to be a dough mixer at a large national bakery and we'd adjust the liquid content of the recipes based on the moisture content in the flour.

"The moisture content of the flour is important for two reasons. First, the higher the moisture content, the lower the amount of dry solids in the flour. Flour specifications usually limit the flour moisture to 14% or less. It is in the miller's interest to hold the moisture as close to 14% as possible."

.
 

BobThe420Builder

Well-Known Member
Same thing with people that have used BAKED pollen. Nobody said rice would not work. But because you didn't follow the process for using flour correctly and were unsuccessful using it you say people are spreading false information. It's not false. Saying flour doesn't work is spreading false information.

Like I said. You need to dry out the flour. If you didn't then you mixed moisture containing flour with your pollen. Flour can contain a lot of moisture. I used to be a dough mixer at a large national bakery and we'd adjust the liquid content of the recipes based on the moisture content in the flour.

"The moisture content of the flour is important for two reasons. First, the higher the moisture content, the lower the amount of dry solids in the flour. Flour specifications usually limit the flour moisture to 14% or less. It is in the miller's interest to hold the moisture as close to 14% as possible."

.
Again your just reading


Try the real life lessons for your self

But ok....yer right I'm wrong...you win
 

CannaOnerStar

Well-Known Member
Ok



And I have real life experience
Me too. Using flour(corn starch) did not make the pollen any less viable. Pollen that i did not mix corn starch with was also viable, but i did not store it as long. I have not tested if it survived taking it off the freezer, using some of the pollen and putting it back. Im sure some of the spores have survived and it can still pollinate.

So i think its safe to say that mixing the pollen with something suitable does not make it less viable, but it might help it store better than only rice. Especially if you open the vial a few times and take it back and forth from freezer. Im sure rice only does the job good enough, at least if you use the whole vial at once.
 

BobThe420Builder

Well-Known Member
I've used this pollen at least 6x, in out, in out, of the freezer

100% success rate in over 18mo

I'm not saying flour dosent work, but I have personally used both over the last 5+ years and rice is much more viable, long and short term period.
 
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