Just waking up so groggy. I'll try to remember to look up the pdf I have Ann's try uploading somewhere.
It uses a sugarless medium so less chance of nasty things taking hold. Second part is I believe there is a better rate of survival in the hardening off stage. I believe it is similar to a method to certain mushroom cultivation where you have air flowing through an aquarium.
It may be too complicated or expensive for a home setup. I haven't been able to set up yet to try it yet unfortunately.
People learned how to germinate orchids seeds in vitro back in 1900 and have experimented since to perfect it and move forward with micropropagation.
I have read that every Cavendish banana is tissue cultured. These are the bananas on shelves world wide. Not surprising, they were bred to have no functional seeds. I'm not sure about other commercial crops.
Venus fly traps and the like ate popular for tc, at least amongst hobbyists and they seem to have that down part as well.
The lack of study on marijuana is because it and hemp have been illegal for so long. There is the one Chinese paper I know of that tried it with hemp. I forget their success rate.
There's only a couple of reasons I see it could be worthwhile other than just liking to tinker and experiment.
1) Keep a back up library of many strains. This in case the mother dies. Or in case you are short on room and want to try new strains still, but don't want to lose other strains you'd need to cull to make room.
2) You're running a large commercial grow. This way you don't need tons of identical mothers to keep product consistency. Even this way you can get variations in size I'd think. My understanding on tissue culture is it's rather uniform in growth when everything is consistent. You wouldn't have to root in multiple batches as the mothers degree enough.
3) Better spread of clone only strains, or maybe hard to clone strains. It theoretically would allow shopping if the person on the other end knew what they were doing. Or to this end possibly the practice of artificial seeds will be perfected for marijuana.
I would say I'm surprised the operations in Amsterdam never picked this up for crop uniformity but sadly I'm not. Those seed companies don't even bother stabilizing their lines. Just grab any two "strains" also them together, also a new name to it and ship it right out the door
Meh, I've run sugarless mediums like in the case of psylosybin cultivation, you can have contamination take hold if the medium has sugar in it or not. It might decrease the likelyhood of infection, so point taken.
Actually the method is not like mushroom cultivation, the byproduct of fungi is CO2, and it does not take much airflow, in fact on the Brown Rice Flour (BRF) cakes I make I do not expose them to circulating air until a fruiting body shows.
So back to "photoautotropic propagation," the idea is that CO2, is the food versus Sucrose in the agar. If we look at Chieri Kubota's work she says that in vitro propagation is slow due to the limited ammount of co2 in vitro. And I somewhat agree after looking at the time it takes from Stage 1 to plant hardening in Wang's or Scroggins work on in vitro cultivation. Off the top of my head from explant to hardened plant takes somewhere around 6 weeks.
Well at the very least we could flood a laminar booth with a high level of co2 when inserting explants into the desired medium. In Kubota's work it looks like there is more mass vs regular explants. And needless to say co2 we know is a basic building block of mass. I'm now sure how I would implement this and mantain a sterile culture at the same time flowing co2 over explants in vitro, not to mention the cost of concentrated co2, as in a compressed cylinder might push me away from going this route.
The abstract you mention is wang's 2009 study on the micropropagation of hemp... Cannabis Sativa L. Same thing if you ask me as the typical cannabis hybrids. The success rate after Stage 3 to hardened plants is 99%.
Well I'm researching this now, I think I'm going to go commercial, and provide clones to commercial grow ops.. I'm looking at pushing clones out in numbers like 200-500 clones a month.
At the very least I've learned a bit, from Kubota's work, I could use the co2 to optimize growth in my explants.
I'll continue to at least look into the subject.
Like.. good stuff man...
