Tissue culture and cannabis?

[MikeGanja]

I need to study the documents in this thread a few more times and perhaps read some literature on basic lab technique/”biotechnology at home” but I will also give it a try.

In the “The Biotechnology of Cannabis sativa” the author included a list with companies that are selling equipment. Most of the products are sold in small quantities to hobbyists. The most expensive equipment I have found so far is a pressure cooker for autoclaving and a small aquarium to build a sterile environment.

Native Humboldt, did you build a glovebox or a flowhood for your setup?

 

[Native Humboldt]

I need to study the documents in this thread a few more times and perhaps read some literature on basic lab technique/”biotechnology at home” but I will also give it a try.

In the “The Biotechnology of Cannabis sativa” the author included a list with companies that are selling equipment. Most of the products are sold in small quantities to hobbyists. The most expensive equipment I have found so far is a pressure cooker for autoclaving and a small aquarium to build a sterile environment.

Native Humboldt, did you build a glovebox or a flowhood for your setup?

I built a flow-hood equivalent to a class II that is basically a HEPA filter built into a recirculating fan that scrubs the air. I also pulled the air through a crushed carbon filtering system. I wish I would have taken pictures of the set-up. I had the clean room set-up inside my shop in the back corner. I think the room was 5x7 and was constructed with 2x4's with a 10 mil layer of clear poly on both sides, ceiling, and floors. The room had a positive pressure created with the HEPA filtered hood. Inside the room I had several set-ups the most successful was a aquarium with filtered air. That's about it besides always wearing clean cloths and removing shoes before entering the clean room. Good luck with your project...

 

[Daub Marley]

If I remember correctly tissue culture does restore the plants original characteristics. Its been a couple of years since I have studied tissue culture but I seem to remember reading that. I'm thinking about trying it out again this year just to see if I can get some plants to survive for a few months and then growing them out while documenting the results. Anyway it sounds fun to give it another shot, you guys inspired me I guess....

Can you explain how that is possible or point me to a reference?

 

[Native Humboldt]

Look it up in the journal of cell biology or just google it. There are several studies on the subject. Like I said its been a while since I have read about the process. In the near future I'll dive back in just too busy right now..

 

[Uncle Ben]

No I have a small sterile home set up, I learned from a tissue culture group a couple of years ago. My wife and I have had really good success with roses and blue berries but I just couldn't get the mix right for cannabis. It would survive for a month maybe 6 weeks then the whole dish would die off. I got frustrated after several tries and lent my set up to a friend who has had a little better success than I had but still isn't there yet. I'm not sure where the $200,000 price tag came from, but yes if I needed one and was motivated I would buy it!

With orchids they use a centrifuge which somehow induces asexual reproduction. They keep spinning/stopping and when they have enough plantlets they let them grow until washed out of the nutrient agar and potted up with tweezer into 3" community pots. They are separated after some growth & then individually potted into 2" pots.

Your setup sounds like quite a feat!

 

[thump easy]

ill run a lil down to you its not hard at all i did it with over the counter nutrients and hormones, i got the sett up from one of those box deals they send you the only fucked up part is the information didnt mach so you'd fuck up because the directions on the video say one thing but the chemicals say another he changed it so your not to shure what the fuck the instructions realy say he has this 1989 vidoe poor footage wich would be great if you could understand it but its not cool a recall would have been great but instead i called the guy and i failed i later seen a few documentaries from univercities on the subject on youtube long and dragging but you get hints and key words like vitamins and hormones one for shoots the other for roots

 

[thump easy]

most important you need a steril air and equipment if you dont got this contamination turns into a percentage of loss i dont care how clean you are you need a lab envirment or contamination is enevitable, and death come with contamination

 

[Native Humboldt]

It would be really nice if 3 or 4 of us put together similar setups and worked together trying different combinations of vitamins along with humidity and temp to achieve the same goal. I'm sure with the plant knowlage and research abilities on this site we could master the process.

 

[thump easy]

but if your technique is on the money you'll have sucess but not totall unless you got everthing on point

 

[thump easy]

thats not the hardest part the hardest part to me is getting them to get an exoscellition or a hardend surface so that they dont go into shock when you pull them out getting them to stableize was the hardeest part for them adapt to the air and climate outside their containers..

 

[Native Humboldt]

thats not the hardest part the hardest part to me is getting them to get an exoscellition or a hardend surface so that they dont go into shock when you pull them out getting them to stableize was the hardeest part for them adapt to the air and climate outside their containers..

Your right but we are dealing with a weed that is pretty forgiving I had great luck with the roses so I'm pretty sure my basic process is correct it just needs to be tuned a bit.

 

[thump easy]

i got the vitamins its so easy your gona be like what the fuck its that easy yes i did the work and im not a high school grad im a GED kid lolz its realy easy i gota find the notes ill give you guys the recipe its so fucken easy no more than 50 buck the jars and the lids probley the most expensive part.. or wait the presure cooker was the expensive part.

 

[thump easy]

give me a day or so today is my day off but my day off means tend the garden..

 

[Sativied]

Nice job thump! I look forward to more info.

I've seen attempts and journals at several forums and they typically end before it becomes interesting.

Some links I bookmarked, can't vouch for the quality, but about cannabis specifically:
http://www.lycaeum.org/mv/anagrams/cannabis_tissue_culture.html
http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf

getting them to stableize was the hardeest part for them adapt to the air and climate outside their containers..

That's the downside of tissue culture many don't realize. Regular cloning is much and much faster.

 

[thump easy]

Nice job thump! I look forward to more info.

I've seen attempts and journals at several forums and they typically end before it becomes interesting.

Some links I bookmarked, can't vouch for the quality, but about cannabis specifically:
http://www.lycaeum.org/mv/anagrams/cannabis_tissue_culture.html
http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf

That's the downside of tissue culture many don't realize. Regular cloning is much and much faster.

thank

Thanks ya the down side is you need money for the equipment and steril everything air ppm purity is expensive and so is the flo hood and all the parts and pieces pluss the room its realy easy its all mostly flora nova veg for the first part i gota find the math but a few drops will do to that pyres glass agar you can get at a health food store for like 9 bucks its rooting hormones for the roots and shoot hormones shit i gota find that paper but ill give you the links of were i got the parts and pieces.. also shoots was an advanced yellow bottle for node production it has a kangaroo on it shit i havent seen that shit hear in the desert but in Whittier California it sells no its not advanced the one we know its from australia it promotes shoot growth in flower durring the first two weeks of flower its bomb for node prodution..

 

[MikeGanja]

It would be really nice if 3 or 4 of us put together similar setups and worked together trying different combinations of vitamins along with humidity and temp to achieve the same goal. I'm sure with the plant knowlage and research abilities on this site we could master the process.

Excellent idea! Sharing information and experiment with different combinations together could save us a lot of time not doing the same mistakes. It could take a while until I have a working sterile setup but I will document and share my experiments on ROI.

 

[MikeGanja]

thump easy: Thank you very much for input and pictures ( and death scene), very inspiring! Do you grow the plants in soil or in hydro during the last period, when they first are exposed to the environment outside the test tube?

 

[thump easy]

thump easy: Thank you very much for input and pictures ( and death scene), very inspiring! Do you grow the plants in soil or in hydro during the last period, when they first are exposed to the environment outside the test tube?

my last try i put them in aroe most died and in coco most died, the way i kept them alive wich was sad was like on or two i cant remember but i put a plastic coke bottle cut with lil holes in them so that they didnt go directly into shock the nutrients in the aroe i just use like aroe clone or aroe ponic chambers is like 200 or less ppms.. i tried and tried and didnt have very good rounds i always work for clinics and pay electricity so i cant skip a beat its got to be a room fool of course with script but tissue culture is very hard without a lab environment, im very smart dont let the spelling get it twisted, i first wash off the agar with ph water just the roots and then introduce them into the cloner with a dome and little holes so the exterior of the plant can harden and acclimatize to the room temp if you let them out bare they will die quick real quick or get sick.. that was as far as i got if i cant fill up the room i cant live eat or drive or anything Edison taxes big time so the success rate is not that great.. but im sure with a sterile environment it can be done with high rates of success.. im sure of it.. im getting offerd to do work like this in the near future but not really interested anymore its to much work.. dont let me ramble on but for the novelist its great fun.. the truth is you do get bigger plants and vigor and if you make it past the first few weeks they become monsters fast..

 

[thump easy]

i used a couple of drops as you could see in the pic with the droper distrilled water agar mixed with a blender of cake blender nutrients spin thier was a small bottled left from the old package called ppm it helps keep your agar steril you could find it on line its a bit pricey but this is what keep you steril most part, a few drops into the steril water and a few drops into water of rooting hormone you want to micro wave it for about five minute move to steril evirments and with clipings already washed for thirty second in bleach water for 30 seconds and thirty seconds in alcohal ya it sounds crazzy but yes this is how you will get rid of all contaminance ... let me start over i gota find the paper work and the right info i just got off work construction rapped it up about 1130 and im tired ill do a real down to the very very incrament of my movements for you guys but hear is the other products now healthy foods agar powder, and and i dont remember the rooting compound i used i always change them around depending if im steril or using benies in my aroe..

 

[AlphaPhase]

it's basically for preserving genetics. If you want 1000 genetics in a small warehouse, tissue culture is where it's at. It works great. I haven't done this myself, but I know people who have. I wouldn't take a tissue culture for something you want to flower next month though, but it does have it's use.