rollinronan
Well-Known Member
the mass transfer of gasses (eg O2) is calculated by the formula Na=Kla(C*-CL) this is the rate at witch a gas passes between phases at the gas/liquid interface
in modern bioreactors there are 2 methods for adding O2 to the mix, 1. sparging (bubbler) and 2. mixing
the reason bubbles are put in the water instead of allowing the water to sit is because it increases the area of the gas/liquid interface; the smallre the bubbles the more area (denoted by "a" in the above formula) where mass transfer can occur, by putting a pump in the water as mentioned above would simulate wave bioreactor designs where rocking is enough to mix the media and provide sufficent gas transfer. rocking may be a little impractical for plants but placing the pump in the resivoir would work as well provided that there is high enough concentration of O2 in the headspace above (CL denotes this factor in the formula and CL denotes total gas in the headspace) to keep up with the O2 requirements of any m/orgs an the plants
Kl is a variable depending on how soluble the gas is in the liquid (O2 in water) aka the mass transfer coefficent
when the solution is mixed the more saturated liquid is moved to the bottom and the unsaturated to the top, its grand in a small setup but nothing huge
in short a pump would be fine for smaller setups but in larger setups the agitation would be insufficent and bubbles would be required
in modern bioreactors there are 2 methods for adding O2 to the mix, 1. sparging (bubbler) and 2. mixing
the reason bubbles are put in the water instead of allowing the water to sit is because it increases the area of the gas/liquid interface; the smallre the bubbles the more area (denoted by "a" in the above formula) where mass transfer can occur, by putting a pump in the water as mentioned above would simulate wave bioreactor designs where rocking is enough to mix the media and provide sufficent gas transfer. rocking may be a little impractical for plants but placing the pump in the resivoir would work as well provided that there is high enough concentration of O2 in the headspace above (CL denotes this factor in the formula and CL denotes total gas in the headspace) to keep up with the O2 requirements of any m/orgs an the plants
Kl is a variable depending on how soluble the gas is in the liquid (O2 in water) aka the mass transfer coefficent
when the solution is mixed the more saturated liquid is moved to the bottom and the unsaturated to the top, its grand in a small setup but nothing huge
in short a pump would be fine for smaller setups but in larger setups the agitation would be insufficent and bubbles would be required