Sterilization
- Thread starter drcartman
- Start date
ndangerspecimen101
Well-Known Member
Pressure cookers are used mainly for exotic mushrooms strains. Most Cubensis strains can be placed in a big pot suspended above the water allowing the jars to be steam cleaned. This is a very viable option if a pressure cooker is not around! 
canndo
Well-Known Member
Um, at the risk of contradicting my friend ndanger, 212 temps will not kill endospores from some very common contaminants. You could boil some of these all day and kill maybe 50 percent of them. And it only takes ONE to spoil your entire endeavor (that depends of course on how many jars you use and how you innoculated etc.)
If you are lucky, you can work with boiling water only and you can get decent results but there are so many ways to destroy your work that it just doesn't make sense to try to avoid a certain and proven method of yielding absolutely sterile substrates and instruments. I have seen them for as little as 15 dollars - they are like 6 quarts or something but you can do a few pints or half pints in them and they will be just fine. If you look at second hand places or salvation army or garage sales you can usually find what you need for very little money. Just make sure the gasket is sound and the weight is included.
If you are lucky, you can work with boiling water only and you can get decent results but there are so many ways to destroy your work that it just doesn't make sense to try to avoid a certain and proven method of yielding absolutely sterile substrates and instruments. I have seen them for as little as 15 dollars - they are like 6 quarts or something but you can do a few pints or half pints in them and they will be just fine. If you look at second hand places or salvation army or garage sales you can usually find what you need for very little money. Just make sure the gasket is sound and the weight is included.
ANC
Well-Known Member
Sounds like you are not in a position in life at which you should be doing this.
ndangerspecimen101
Well-Known Member
True.
But a friend of a friend conducted such trials in a big pot allowing steam sterilization with success. Although, its best to carry out plans by using a pressure cooker by eliminating bacteria effectively. I deeply respect your post!
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weasels911
Well-Known Member
If you are just following the PF Tek, you can get away with boiling water in a big pot. I used sterilize like that with pretty good results. If you want to go further with the hobby, a pressure cooker IS necessary. Don't waste your time trying to do grains without one.
sonar
Well-Known Member
Yes, if you are just doing brf cakes you can use a pot of boiling water as per the PF Tek.