Slow/No signs of colonization

thetester

Active Member
I've got a batch of BRF PF-Tek. They have all have been colonizing very very slowly which I suspect is because the spores were shipped during that whole polar vortex business. I've got one that after 16 days has absolutely no sign of colonization. How long would you wait before giving up and dumping it?
 

canndo

Well-Known Member
If your temperatures are decent, and you don't see growth in that amount of time, you are done.
 

thetester

Active Member
I forgot to provide that bit of info. It has been very cold here lately, it averages around 73 degrees in the place I have them stored while they colonize.
 

dayton937toker

Well-Known Member
They would usually show in the 1st week or so. My first run Showed In 5 Days. Did U Mix The Sub Right..sterilize. Flame Between Jars? U Want Moist Cakes Not Drippin Wet
 

MustangStudFarm

Well-Known Member
Drop the BRF and go bulk sub... rye to colonize and hay/cow manure in mono tubs... PM me if interested... I am running cambodian with great sucess... I tried BRF but found it more trouble than yield, also very slow to colonize... my Cam likes it about 85F, very fast speed all around...
 

canndo

Well-Known Member
Pf is neither easier nor mor reasonable than grain. You can do anything with grain from spawn, to g2g expansion to simply casing the stuff. You can get any amount of grain to colonize in a week or so, any amount. Why would anyone use anything else?
 

tylerrrrr

Well-Known Member
Drop the BRF and go bulk sub... rye to colonize and hay/cow manure in mono tubs... PM me if interested... I am running cambodian with great sucess... I tried BRF but found it more trouble than yield, also very slow to colonize... my Cam likes it about 85F, very fast speed all around...
85 is way too hot. Mycelium makes its own heat so really the jar is around 90 or so.
 

thetester

Active Member
I like doing pf-tek because I can spot contamination early on and if I do have a problem my entire batch isn't ruined. I haven't found a clear explanation on how to do grain bulk grow.
 

canndo

Well-Known Member
I like doing pf-tek because I can spot contamination early on and if I do have a problem my entire batch isn't ruined. I haven't found a clear explanation on how to do grain bulk grow.
Spotting contams in grain is very easy. If you are doing more than one jar or bag - you have a safe backup - and even one jar fully colonized can be expanded manyfold. Besides, the time factor is such that you don't have to wait nearly so long. Also, it iis a lot less work.
 

Kervork

Well-Known Member
78-80 degrees. Always start with a liquid culture. PF Tek is silly, mushrooms were born to grow in manure, all efforts should be torwards eventually innoculating compost. PF tek hard, bullshit easy.
 

thetester

Active Member
So here is an update. The one showing no signs of colonization did get going and is colonizing now. The others in the batch are moving along now too. It took 15 days for that one jar but all's well that ends well I guess. I guess my next project will be to build a temperature controlled closet. I can't keep my heat too much higher in the winter, I'm not rich and the electric bill is a bitch. Fortunately for me, spring, summer and fall present no temperature problems for me.
 
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