My Clone From Leaf Experiment
- Thread starter MsMILFweed
- Start date
kevtherev
Active Member
tissue culture is real and you can get plants from leaf or stem. I might reaseach as i hate to cut clones of plant im going to bud. But due to the law of the land of oz i only grow one at a time or you get busted bad. With one a slap on the wrist and a few bucks to poor box. Ive started first hydro so its all yer round save me getting ripped off and dealing with the scummy dealers.
iMThaWeedMan
Active Member
thats truly amazing!
We TaRdED
Well-Known Member
TetraHyC
Well-Known Member
I concur, next vote.
So this punk Woo, is sneaking around, new handle.
Hey Woo, like hiding behind your computer, be a man log on using Woo
Rob Street
Active Member
always wondered if that would work. 
MsMILFweed
Well-Known Member
lol What the Woo indeed!
WWWD? What would Woo Do?
or
Where in the World is Woo?
I'm disappointed, I'd like to have seen this technique work. While I believe it can be done (esp if you were to go the tissue culture route), the whole reason for trying this was because it was supposed to be better/easier/faster than taking clones the standard way. At least that was how the Woomeister sold it to us.
Well, I still can't see any benefit to this way (if it does work) to standard cloning techniques, where I can get roots in less than a week.
uberpea
Well-Known Member
MsMILFweed
Well-Known Member
Maybe Woo's original thread was an experiment to see how many dumbfuckwits he could get to fall into his trap to try and test his idea.
... well he got me.
... well he got me.
bicycle racer
Well-Known Member
its important to attempt new things. there is no loss if something is learned in the process whether succesfull or not
BlessAmerica
Well-Known Member
Hey Guys,
I know that MsMILF busted the myth, but I must add.
I too tried this. I took two clones (need to post a pic of them), and as an experiment (before hearing of this) decided to try the leaf too.
I cut the leaf off at a 45 degree angle, and set it at a 45 degree angle in the soil so the leaf would not rot. I placed it in with the other two clones and waited.....
Two weeks later the clones had developed roots, and the leaf had developed a ball with a very thick resemblance of a root. Then about a week later the leaf began to yellow, and shortly died therafter.
So I agree with MsMILF, BUSTED!
I know that MsMILF busted the myth, but I must add.
I too tried this. I took two clones (need to post a pic of them), and as an experiment (before hearing of this) decided to try the leaf too.
I cut the leaf off at a 45 degree angle, and set it at a 45 degree angle in the soil so the leaf would not rot. I placed it in with the other two clones and waited.....
Two weeks later the clones had developed roots, and the leaf had developed a ball with a very thick resemblance of a root. Then about a week later the leaf began to yellow, and shortly died therafter.
So I agree with MsMILF, BUSTED!
Hey all
well, didnt have time to read the ten middle pages of this thread, but...I would like to add my two cents.
So, i've been working in tissue culture for the past two years, and...this is very possible. the things is, fan leaves are (probably) too old to display the type of totipotency (the ability for a single cell to regenerate into a whole new plant) that is needed.
Here is how you would do it:
Grow your seed until it is the size of the plants in my avatar. basically you want cotyledons fully grown, with the first true leaves JUST beginning to appear/less than half a centimeter.
at that point you cut off the cotyledons, you'll want to cut off the very end, about 2 or 3mm of the end of the cotyledon.
now, I use aseptic agar and sterile conditions to do this...but it should be possible in soil. you'll want to supply a very specific ration of auxin to cytokinins, my magical 'shoot regeneration formula' is 2 milligrams/liter cytokinins (I use trans-zeatin-riboside) and 0.1 milligram/liter auxin (I use I.A.A, Indole-3-acetic acid) after a few weeks a very small callus will appear on the cut ends of the cotyledon, and a bunch of new shoots will start growing out of the cuts.
at that point you cut the shoots off the cotyledon and transfer them to new media, one that has the right formulation to cause roots to grow. I use 5 milligrams/liter I.A.A, thats all.
in another few weeks you should have roots forming.
It's actually pretty simple. well, it's pretty simple when you've got a lab, autoclave, agar, powdered plant hormones, etc.
I'll post some pictures of this process done with tomatoes
Appropriately aged plants for totipotent regeneration:
Cotyledons and hypocotyls (stems) regenerating callus and then shoots:
After the shoot has gotten big enough, you put it on the rooting media:
Sorry, I dont have any pics on this PC of the actually rooted plants...but i've grown plenty of them out to the point where they can be transplanted into soil, and they grow up and become whole new plants, producing fruit.
so, this can be done. it's not some "stick it in dirt and pray" thing...I doubt that would work, but, it can be done.
well, didnt have time to read the ten middle pages of this thread, but...I would like to add my two cents.
So, i've been working in tissue culture for the past two years, and...this is very possible. the things is, fan leaves are (probably) too old to display the type of totipotency (the ability for a single cell to regenerate into a whole new plant) that is needed.
Here is how you would do it:
Grow your seed until it is the size of the plants in my avatar. basically you want cotyledons fully grown, with the first true leaves JUST beginning to appear/less than half a centimeter.
at that point you cut off the cotyledons, you'll want to cut off the very end, about 2 or 3mm of the end of the cotyledon.
now, I use aseptic agar and sterile conditions to do this...but it should be possible in soil. you'll want to supply a very specific ration of auxin to cytokinins, my magical 'shoot regeneration formula' is 2 milligrams/liter cytokinins (I use trans-zeatin-riboside) and 0.1 milligram/liter auxin (I use I.A.A, Indole-3-acetic acid) after a few weeks a very small callus will appear on the cut ends of the cotyledon, and a bunch of new shoots will start growing out of the cuts.
at that point you cut the shoots off the cotyledon and transfer them to new media, one that has the right formulation to cause roots to grow. I use 5 milligrams/liter I.A.A, thats all.
in another few weeks you should have roots forming.
It's actually pretty simple. well, it's pretty simple when you've got a lab, autoclave, agar, powdered plant hormones, etc.
I'll post some pictures of this process done with tomatoes
Appropriately aged plants for totipotent regeneration:
Cotyledons and hypocotyls (stems) regenerating callus and then shoots:
After the shoot has gotten big enough, you put it on the rooting media:
Sorry, I dont have any pics on this PC of the actually rooted plants...but i've grown plenty of them out to the point where they can be transplanted into soil, and they grow up and become whole new plants, producing fruit.
so, this can be done. it's not some "stick it in dirt and pray" thing...I doubt that would work, but, it can be done.