interesting, pertaining to hops: 
original thread: http://www.homebrewtalk.com/f92/purple-hops-143398/
it makes you wonder..
original thread: http://www.homebrewtalk.com/f92/purple-hops-143398/it makes you wonder..
marijuana mad science (grafting marijuana to other plants)
- Thread starter Rhyspect
- Start date
that dizzy looks like what happens to plants when you mutate them with roundup.
i will get you pictures of what it did to a small elm tree one of these days. small, distorted leaves and lots of em. has stayed that way for years too. looks pretty cool. i would love to have some of that stuff because nobody would murder it as long as you didnt put it where they weed or cut.
i will get you pictures of what it did to a small elm tree one of these days. small, distorted leaves and lots of em. has stayed that way for years too. looks pretty cool. i would love to have some of that stuff because nobody would murder it as long as you didnt put it where they weed or cut.
i have always wanted to engineer a fruiting moss that is really just a microweedforest.
this might start with creating haploid cannabis plants(in plant tissue culture/micropropagation), something that interests me in the first place. haploid a male, haploid a female, observe their characteristics, test each to see if it gets you high, etc. plus all that selecting the ultimate genetics and creating 100% females like i mentioned earlier.
http://en.wikipedia.org/wiki/Doubled_haploidy
how i propose to make truly 100% female seeds with the ultimate genetics
Conventional inbreeding procedures take six generations to achieve approximately complete homozygosity, whereas doubled haploidy achieves it in one generation.[1] Hence, artificial production of doubled haploids is of a great importance in plant breeding.
this might start with creating haploid cannabis plants(in plant tissue culture/micropropagation), something that interests me in the first place. haploid a male, haploid a female, observe their characteristics, test each to see if it gets you high, etc. plus all that selecting the ultimate genetics and creating 100% females like i mentioned earlier.
http://en.wikipedia.org/wiki/Doubled_haploidy
how i propose to make truly 100% female seeds with the ultimate genetics
Conventional inbreeding procedures take six generations to achieve approximately complete homozygosity, whereas doubled haploidy achieves it in one generation.[1] Hence, artificial production of doubled haploids is of a great importance in plant breeding.
That 5hit
Well-Known Member
thats not grafting
grating does not impart a part of one plant onto another plant
all it does is allow one plant to live off another plant while staying 2 differant plants
you cant graft an apple tree onto an orange tree and think you going to make a brand new friut
what you are talking about is a gene crossing a splicing
this is only done at the cell level
try puting differ pollins on the flowers to see if there will be any seeds
but grafting the stem onto onther plant does nothing .LOL
grating does not impart a part of one plant onto another plant
all it does is allow one plant to live off another plant while staying 2 differant plants
you cant graft an apple tree onto an orange tree and think you going to make a brand new friut
what you are talking about is a gene crossing a splicing
this is only done at the cell level
try puting differ pollins on the flowers to see if there will be any seeds
but grafting the stem onto onther plant does nothing .LOL
yes, i have given up on grafting. if you know what 'kudzu' is, i use to attempt to graft all kinds of food plants to kudzu, except beans/legumes, which kudzu is most closely related to. i am talking about using some high-tech methods that require sterile environment and special equipment. and some lower-tech experiments, such as chopping up 2 or more different plants, and letting them grow together in tissue culture. \ although if i had some specialized equipment, i would go even farther, experimenting at the cellular level to create new plant species that wouldnt have the possibility of seperating back into 2 (or more) different plants later.
methods which would require a verrrry small needle and a very good microscope.
then there is the method where you 'graft' seeds. taking the embryo or some plant material from a cannabis seed and putting it into a hops seed, and vice versa. it has been done, several years ago, with a type of wheat and a type of maize, to create a plant that grew pieces of wheat plant and pieces of corn plant.
but as far as normal grafting, i gave up on that. i have seen a thread in a forum, with pictures, where a guy grafted cannabis on either hops or grapevine. but he had to keep the junction moist, he basically had a piece of budplant cut and taped to a cut vine, and was keeping it on lifesupport by watering the graft union, where eventually it was supposed to heal and become an actual graft union, and the plant could finally get its water and nutrients from the host.
the kind of grafting i want to experiment with in the future, is where you micropropagate cuttings in tissue culture, letting different plants touch, and sometimes at the junction, you get a shoot of a new plant that is the combination of the two. it has been done. so even if i dont ever get around to creating new fruits and flowers, all with cannabinoid DNA, i will create chimeras. most likely for ornamental purposes, unless i made a cocapoppyweed chimera..
i dont have any of this equipment, lab, or access to them, if i attempted now, it would be akin to growing shrooms in jars with kitchen equipment. so for now, it is just a dream i have, that will not begin to take place until at least 2015 for me, but is entirely possible for anyone with access to the proper equipment/funding, and i say go for it, as an experimental hobby that could lead to great things.
AFAIK, micropropagation, plant tissue culture is the only way to get plants that normally wouldnt graft to each other, to do so.
and theres a way to help them graft, by fusing their cellular membranes together via electrolysis, or passing an electrical charge between the two different plants, after cutting and placing together. there may have been some special skinning of the cells that make contact before passing the electricity through, which transports dna from one side to another, and helps them fuse, because the dna getting into the host helps calm down the immune response that would normally fight off the new plant and make the graft fail.
i wish i could find some links for you, but these things are buried in google under years of new shit. i cant even find the paper about the guy who was using colchicine and GA3 on cannabis to make a better hemp, and ended up making superweed and a vinelike sativa.
i did, however, find this research that says GA3 decreased THC content:Four-week-old Cannabis sativa L. plants were treated with 1 ml. of gibberellic acid or indoleacetic acid at concentrations up to 250 p.p.m. The treatment was repeated at weekly intervals for 3 weeks. These plants were harvested 1 week after the last treatment. Gibberellic acid caused a significant increase in height and a decrease in the weight of leaves and
9-tetrahydrocannabinol content. Indoleacetic acid produced no significant changes.
methods which would require a verrrry small needle and a very good microscope.
then there is the method where you 'graft' seeds. taking the embryo or some plant material from a cannabis seed and putting it into a hops seed, and vice versa. it has been done, several years ago, with a type of wheat and a type of maize, to create a plant that grew pieces of wheat plant and pieces of corn plant.
but as far as normal grafting, i gave up on that. i have seen a thread in a forum, with pictures, where a guy grafted cannabis on either hops or grapevine. but he had to keep the junction moist, he basically had a piece of budplant cut and taped to a cut vine, and was keeping it on lifesupport by watering the graft union, where eventually it was supposed to heal and become an actual graft union, and the plant could finally get its water and nutrients from the host.
the kind of grafting i want to experiment with in the future, is where you micropropagate cuttings in tissue culture, letting different plants touch, and sometimes at the junction, you get a shoot of a new plant that is the combination of the two. it has been done. so even if i dont ever get around to creating new fruits and flowers, all with cannabinoid DNA, i will create chimeras. most likely for ornamental purposes, unless i made a cocapoppyweed chimera..
i dont have any of this equipment, lab, or access to them, if i attempted now, it would be akin to growing shrooms in jars with kitchen equipment. so for now, it is just a dream i have, that will not begin to take place until at least 2015 for me, but is entirely possible for anyone with access to the proper equipment/funding, and i say go for it, as an experimental hobby that could lead to great things.
AFAIK, micropropagation, plant tissue culture is the only way to get plants that normally wouldnt graft to each other, to do so.
and theres a way to help them graft, by fusing their cellular membranes together via electrolysis, or passing an electrical charge between the two different plants, after cutting and placing together. there may have been some special skinning of the cells that make contact before passing the electricity through, which transports dna from one side to another, and helps them fuse, because the dna getting into the host helps calm down the immune response that would normally fight off the new plant and make the graft fail.
i wish i could find some links for you, but these things are buried in google under years of new shit. i cant even find the paper about the guy who was using colchicine and GA3 on cannabis to make a better hemp, and ended up making superweed and a vinelike sativa.
i did, however, find this research that says GA3 decreased THC content:Four-week-old Cannabis sativa L. plants were treated with 1 ml. of gibberellic acid or indoleacetic acid at concentrations up to 250 p.p.m. The treatment was repeated at weekly intervals for 3 weeks. These plants were harvested 1 week after the last treatment. Gibberellic acid caused a significant increase in height and a decrease in the weight of leaves and
hmm a strawberry stoner milkshake? 0.o
veggiegardener
Well-Known Member
The basic research on grafting Cannabis was done many years ago.
The results:
Hops(a close relative of Cannabis) grafted to Cannabis does not begin making THC.
Cannabis, grafted to Hops roots, produces THC.
Neither half of the graft changes its innate growth pattern.
I figure some bright kid will do some genetic engineering and we'll see "superpot" sooner or later.
200% THC, including the roots.
Finishes from seed, yesterday.
Tastes like perfection.
Very mild smoke.
Bestows immortality.
Hurry up, Damn it!
The results:
Hops(a close relative of Cannabis) grafted to Cannabis does not begin making THC.
Cannabis, grafted to Hops roots, produces THC.
Neither half of the graft changes its innate growth pattern.
I figure some bright kid will do some genetic engineering and we'll see "superpot" sooner or later.
200% THC, including the roots.
Finishes from seed, yesterday.
Tastes like perfection.
Very mild smoke.
Bestows immortality.
Hurry up, Damn it!
B2K
Member
Haha man this is crazy, my buddy has been working on attempting this. He learned about it in one of his horticulture classes and tried to graft a branch from one strain onto the stalk of a different strain and it didn't work out. He's not pro on it yet though, over the summer he's taking a class specifically on the grafting of plants over the summer so I'll make sure to make a post to let you guys know how it works out!
parabear
Member
Shit I would find a giant tree, and graft like 30 plants to it then, so when the policia bitched i am exceeding the limit for medical plants, i'll point and say, 'but officer, there is only one plant there, at most 2!!!'. Wonder what kinda yield an outdor grow like that would have.... hmmm... I feel an experiment... need to find big shrubs or small trees with great root characteristics... hmmmmm
parabear
Member
No it will be the columbians, altering marijuana to produce cocaine alkaloids like coca leaf, in addition to THC... they will fuck it up for us all...
On a serious note, yu need to look at the fact strawberries do not grow as big a root system as MJ does. That makes it a poor choice. You want a root system that can uptake the nutrients at the proper pressure ranges, and optimal rates. I mean I am sure it is possible to get a marijuana plant to grow to 30 ft tall (sativa) with optimal conditions... but we need the research to be done in order to optimize the plants. If we could breed a strain that would grow/flower faster, and yield a shit ton, then by all means lets get crackin' on it.
parabear
Member
yes, i have given up on grafting. if you know what 'kudzu' is, i use to attempt to graft all kinds of food plants to kudzu, except beans/legumes, which kudzu is most closely related to. i am talking about using some high-tech methods that require sterile environment and special equipment. and some lower-tech experiments, such as chopping up 2 or more different plants, and letting them grow together in tissue culture. \ although if i had some specialized equipment, i would go even farther, experimenting at the cellular level to create new plant species that wouldnt have the possibility of seperating back into 2 (or more) different plants later.
methods which would require a verrrry small needle and a very good microscope.
then there is the method where you 'graft' seeds. taking the embryo or some plant material from a cannabis seed and putting it into a hops seed, and vice versa. it has been done, several years ago, with a type of wheat and a type of maize, to create a plant that grew pieces of wheat plant and pieces of corn plant.
but as far as normal grafting, i gave up on that. i have seen a thread in a forum, with pictures, where a guy grafted cannabis on either hops or grapevine. but he had to keep the junction moist, he basically had a piece of budplant cut and taped to a cut vine, and was keeping it on lifesupport by watering the graft union, where eventually it was supposed to heal and become an actual graft union, and the plant could finally get its water and nutrients from the host.
the kind of grafting i want to experiment with in the future, is where you micropropagate cuttings in tissue culture, letting different plants touch, and sometimes at the junction, you get a shoot of a new plant that is the combination of the two. it has been done. so even if i dont ever get around to creating new fruits and flowers, all with cannabinoid DNA, i will create chimeras. most likely for ornamental purposes, unless i made a cocapoppyweed chimera..
i dont have any of this equipment, lab, or access to them, if i attempted now, it would be akin to growing shrooms in jars with kitchen equipment. so for now, it is just a dream i have, that will not begin to take place until at least 2015 for me, but is entirely possible for anyone with access to the proper equipment/funding, and i say go for it, as an experimental hobby that could lead to great things.
AFAIK, micropropagation, plant tissue culture is the only way to get plants that normally wouldnt graft to each other, to do so.
and theres a way to help them graft, by fusing their cellular membranes together via electrolysis, or passing an electrical charge between the two different plants, after cutting and placing together. there may have been some special skinning of the cells that make contact before passing the electricity through, which transports dna from one side to another, and helps them fuse, because the dna getting into the host helps calm down the immune response that would normally fight off the new plant and make the graft fail.
i wish i could find some links for you, but these things are buried in google under years of new shit. i cant even find the paper about the guy who was using colchicine and GA3 on cannabis to make a better hemp, and ended up making superweed and a vinelike sativa.
i did, however, find this research that says GA3 decreased THC content:Four-week-old Cannabis sativa L. plants were treated with 1 ml. of gibberellic acid or indoleacetic acid at concentrations up to 250 p.p.m. The treatment was repeated at weekly intervals for 3 weeks. These plants were harvested 1 week after the last treatment. Gibberellic acid caused a significant increase in height and a decrease in the weight of leaves and9-tetrahydrocannabinol content. Indoleacetic acid produced no significant changes.
the seed grafting is also known as chimera production. It has also fund its way into ornamental plants, producing exotic plants, as has grafting. Besides, wouldn't a lettuceweed hybrid with a good high THC content be the best thing ever?
rollinronan
Well-Known Member
since u mention it
genetics is my feild in the profesional world
i was thinking (a lasy way of sayin it) take the genes for producing the trichomes and resin.......put them onto tobacco
its legal (till ur found out) ....smells good and looks 100% inconspicus
completly theory tho
would need alot of work and equipment
as for grafting YES it can be done ..... its standard practice with the likes of apple trees and pears
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