Procedure
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Substrate Preparation
Take the lids from the ½ pint jars and use the drill or hammer and nail to poke holes in them. One hole per lid is sufficient, however some people prefer to use 2 or 4 holes, so they can inoculate the cake in more than one spot.
This has the advantage of faster colonization of the cake, since growth starts in several places. The disadvantage is that every time the syringe needle goes into the cake, there is a chance for contamination. So wipe the syringe with rubbing alcohol before inoculating every hole.
Most guides suggest using 4 holes. Make sure that the holes are not too close to the edge of the lid. About 3/4" (2 cm) in from the edge is sufficient.
Holes In The Canning Lids
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Mix Up The Substrate
Using the appropriate quantities as outlined in the table below, mix the vermiculite and brown rice flour together in the mixing bowl, using the large spoon. Add the water and mix thoroughly.
This will take some patience and will probably wear out your arm a bit. If you are concerned about the purity of the tap water in your area, you may want to used distilled water rather than tap water.
In most cases, though, tap water is fine. The recipe below often comes out just a bit more than what you need for the jars.
Simply discard any extra substrate. Don't try to pack it into the jars, because you want the substrate to be airy and fluffy for optimal growth.
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The Amount Of Material For Each Jar Is
Vermiculite --- 2/3 cup (5.25 ounces)
Flour --- 1/4 cup (2 ounces)
Water --- 1/4 cup (2 ounces)
These amounts are for 1/2 pint (about 8 ounces or 250 ml) jars. Adjust the amounts accordingly if you use different size jars. For example if you doubled the size to 1 pint (about 16 ounces or 500 ml) jars, you would need double the amount of material for each jar.
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Add Substrate To The Jars
Using the mixing spoon, fill each jar to within ¾" (2 cm) of the top of the jar. It is very important that the substrate mix be open and airy, not packed into the jar. Dump it in, shake any excess back into the bowl, but don’t pack it down at all. Mycelium will grow best in all those little open spaces.
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Seal The Jars
Carefully wipe clean the exposed inner wall of the jar and the rim. Fill to the top with clean dry vermiculite, screw on the lid and band tightly, and cover the lid with a large square of aluminum foil, to prevent water droplets from entering.
Optionally, breathable tape such as cloth surgical tape, can be placed over the holes. Your jar is sealed and ready for sterilization.
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Sterilizing The Jars
Place the finished jars in your pressure cooker or pressure canner, and cook them at 15psi for 45-60 minutes, according to the instructions for your pressure cooker/canner. Let the entire apparatus cool completely.
Do not try to open the pressure cooker before it is cool to the touch, and do not try to speed the cooling process, as a quick change in temperature could crack the jars.
Be sure to let the jars cool for several hours, because heat is often trapped in the center of the cake, even if the jar feels cool. This heat can kill your spores if you try to inoculate too soon after cooking.
It’s best if you can let your jars cool overnight. The jars are now sealed containers of sterile substrate. If you have done everything correctly, they can be stored indefinitely until you are ready to use them.
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Steam Sterilization
I didn't have a pressure cooker or pressure canner the first time I grew magic mushrooms and I didn't want to spend money until I knew that my mushroom crop would be successful. So the steaming process was used for sterilization.
Any pot with tight closing lid, which can hold sufficient jars is fine. You want a hot environment that will kill germs in the jar but not add or remove water from the jars you are sterilizing.
In short, water is added to a pot, something is placed in the pot to raise the jars from touching the bottom and keep water from entering the jars. Jars are placed inside, and water is boiled for an hour or more.
First, you will need something to keep the bottom of the jars from touching the bottom of the pot. A three-piece vegetable steamer (pot, basket insert and lid) will do. Stainless steel vegetable steamers that fold out and stand on the bottom of the pot can also be used. You can even use small rocks or marbles. Then place the jars on top.
Add enough water to the bottom of the pot to cover about one half inch of the bottom of the jars. The layer of water on the bottom of the pan may touch the jars; care should be taken however to prevent boiling water from entering the jars.
Cover the top of the jars with a piece of aluminum-foil and tighten it with a rubber band. This will help prevent any water from boiling up and entering the jars. The extra water would increase the moisture content too much.
Gently steam the jars by boiling for an hour in a tightly covered pot. Keep the bottom of the pot filled with water by checking the water level and adding water as the steam evaporates.
A good tight fitting pot lid is essential for successful steaming. Be careful not to overheat the jars, as the substrate will dry out. A gentle constant boil is what is needed. The jars can be inoculated as soon as their inside temperature has dropped below 35°C (95°F).
I have had a 100% success rate using this method. I have also heard of people getting 100% contamination using this method. I think the difference is due to the fact of my taking a shower before working on all of the mixing and inoculation work. I then work while in the bathroom with just a clean towel on.
I also clean the inside and outside the jars with rubbing alcohol after drilling the caps, then sealing them.
I sterilize everything with rubbing alcohol before using anything that will come in contact with the inside or outside of the jars. That means tables, mixing bowls, measuring cups, hands, etc. The water that is added to the cakes is boiled and cooled before using.
If you don't have access to a pressure cooker or canner, be extra sterile and you can have 100% clean cakes.
Inoculation
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Cleanliness Precautions
Inoculating your jars is the main step where contamination is possible, and thus must be done in as clean of an environment as possible. If the room you’re working in is clean enough, you can get away with inoculating them in open air.
The needle of the syringe, if not absolutely sterile, can carry bacteria and spores from other molds into your cake, contaminating and ruining the cake. Wash your hands and face with antibacterial soap. Wear clean clothes. Anything in the area of the syringe and jars could contaminate your cakes if it is not clean.
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Glove Box (Optional)
If you’re concerned about sterility, a good way to accomplish this is to make a 'glove box', an enclosed, semi-sealed box with holes for gloves to go through and a see-through top.
A cheap, halfway decent one can be built for only a couple bucks worth of stuff. All you need is a large cardboard box, some tape and saran wrap to go over the top of the box, and a pair of new, unused dishwashing gloves.
Tape saran wrap over the top and cut two holes big enough for your arms in the sides. Disinfect the gloves and the inside of the box with Lysol spray disinfectant.
A small gate can be cut into the side of the box for getting the syringe and jars into the box, or they can be put through one of the arm holes (if you choose not to attach the gloves to the holes).
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Oven Inoculation: Cleanliness Simplified
If you have an oven at your disposal, forget all about glove boxes and all that troublesome nonsense. The simplest, easiest way to assure cleanliness during inoculation is to do it on an oven rack. Turn your oven on at the lowest possible setting, and let it heat up.
Once it's preheated, pull one of the racks out as far as it will go without falling out of the oven. (Use a rack near the bottom) Have your jars and spore syringe all nearby, ready to go. Place 3 or 4 jars at a time on the edge of the oven rack, and begin carefully inoculating them with the syringe.
It's a good idea to have a lighter handy as well to sterilize the needle as you go. Flame the needle until it gets very hot, then carefully squirt a little bit of spore solution (if you can spare it) to cool down the needle before sticking it in the cake. Putting a hot needle into the cake will get burnt-on rice flour all over the needle.
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Spore Injection
Once you’re ready to inoculate, shake up the spore syringe to get as many spores as possible off the sides of the syringe and into the water. Carefully remove the cap over the syringe needle and slide the needle into one of the holes in the jar lid.
Shove it all the way in, so that the needle goes into the cake itself. Gently squeeze out about 0.5 to 1.0 cc of spore solution into each jar, splitting up the amount if you inject through more than one hole. Some people suggest using an entire cc of solution per jar, however I have had great success with only 0.5cc each.
Be careful that nothing but the jar and substrate touch the needle, and re-cap it immediately after using it to avoid contaminating the needle. Also be careful of using too much spore solution.
With spore syringes it can be easy to accidentally push the plunger on the syringe too forcefully and dump out way too much solution. Once each jar is inoculated, it is ready for incubation. Put tape over the holes in the lid to keep out any contaminants.
Mycelial (Vegetative) Growth
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Incubation
Now the jars are incubated at about 75-85 degrees F, in a dark place, for several weeks. If you have a room that is constantly kept in this general range, this is a good place to incubate your jars.
If not, you will need to find some other source of heat to keep them in that temperature range. Be careful not to use any heat source that could cause fires; a heating pad will usually work, some people have used fish tank heaters submerged in a warm water bath.
A good investment here is a thermometer that keeps track of highest and lowest temperatures, so you can see how hot or cold your cakes are getting.
If they get too cold, their growth will slow considerably, and if they get too hot, they will lose water and eventually die. (They will usually die if they ever get above 95 degrees F)
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Mycelial Growth
The first signs of mycelial growth should appear within 5-7 days. If none appear within two weeks, something went wrong. (Perhaps the cake was not cooled completely before inoculation, and the heat killed the spores, or the spores simply did not make it into the cake).
This type of mushroom mycelium will always be a brilliant white fuzz, often growing in ropy strands. This ropy type of growth is called rhizomorphic growth, and is a sign that the mycelium will probably fruit very well.
Any other color of mold, including some less brilliantly white molds (cobweb mold, for example, is white but not so thick, and it does look a lot like cobwebs.), is a sign of contamination. A contaminated cake will not recover and, except in very rare instances, will never produce mushrooms.
After colonization is complete it is time for
fruiting the magic mushrooms.