Cloning from Fan Leaves

mindphuk

mindphuk

Well-Known Member
I don't think it's impossible because there is still a lot of new information that botanists are learning. Just because it hasn't been done isn't evidence that it can't be.
As many of you know, unlike people, plants have stem cells in their roots and stems their whole life. It is pretty current research where findings refining their knowledge of signaling pathways that define stem cell fate and specify either shoot or root stem cell function.

It appears that ethylene plays a role in that function so even though our traditional cloning methods may not work, there may be other methods, possibly using small amounts of ethylene gas (pretty toxic stuff to inhale, so not something to experiment with at home).
http://news.bio-medicine.org/biology-news-3/Scientists-find-stem-cell-switch-295-1/

One possibility would be to do the opposite of what we have been and that would be to take a cutting from the root itself and try to get the exposed area to differentiate into a stem and leaves.
 
Syriuslydelyrius

Syriuslydelyrius

Well-Known Member
mindphuk said:
I don't think it's impossible because there is still a lot of new information that botanists are learning. Just because it hasn't been done isn't evidence that it can't be.
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Taking a fan leaf diping it in root tone and doing doing your best to root and grow it in the same manner as we have been doing with clones sence the dawn of time. I would still dare to call impossible if not for some miricle freak of nature. Yes there is alot of new information becoming known to the world and the future will bring along much more knowledge and results. However if some such a breakthrough does come to exist that will allow for not only more but faster cellular differentiation/growth but more of it! There is still the fact that a clipping has more cell mass to start with than 1 single leaf would still put it much farther ahead.
 
mindphuk

mindphuk

Well-Known Member
Syriuslydelyrius said:
Taking a fan leaf diping it in root tone and doing doing your best to root and grow it in the same manner as we have been doing with clones sence the dawn of time. I would still dare to call impossible if not for some miricle freak of nature. Yes there is alot of new information becoming known to the world and the future will bring along much more knowledge and results. However if some such a breakthrough does come to exist that will allow for not only more but faster cellular differentiation/growth but more of it! There is still the fact that a clipping has more cell mass to start with than 1 single leaf would still put it much farther ahead.
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Yep, that's why I said our traditional cloning methods would not work.

I may have misread the OP because I didn't think he meant to take a single leaf but try to root the stem the fan leaves are attached to.

This is pretty much all academic anyway since we already have a viable easy cloning methods, there really is no practical reason I can think of that would make this advantageous over taking a regular cutting.
 
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born2killspam

Well-Known Member
I wasn't being a passive agressive troll, don't worry.. I've covered pretty much everything you said in my own reading, but one other thing I've read a number of times is that all plant cells are capable of undifferentiating, and then differentiating again.. I think you overlooked the 're'..
Perhaps tissue culture has an advantage over trying to root a fan leaf since fewer cells need to be undifferentiated/redifferentiated to may way for new cells to grow and differentiate?..
 
Syriuslydelyrius

Syriuslydelyrius

Well-Known Member
I will try to keep it breif as the natives seem to be getting rather restless on this post.

born2killspam said:
I think you overlooked the 're'..
Perhaps tissue culture has an advantage over trying to root a fan leaf since fewer cells need to be undifferentiated/redifferentiated to may way for new cells to grow and differentiate?..
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Cellular differentiation would be the proper definition or term used and the undifferentation and redifferentation of cells is still referd to as cellular differentiation. As the cell structure is or has changed into a new one. If cells defferentiated into a new cell type then later it went back to orignal yes I suppose you can refer to that as redifferentation back to the orignal cell type but it still had to have differentated to to become that way hence the term cellular differentation.

born2killspam said:
I've read a number of times is that all plant cells are capable of undifferentiating, and then differentiating again..
Click to expand...
A plant cell that is capable of differentation is refered to as a pluripotent cell. If all of plant cells are capable of differentation its the level of that capability or pluripotency that becomes the factor. Can these cells "morph" enough to make the needed change?


born2killspam said:
Perhaps tissue culture has an advantage over trying to root a fan leaf since fewer cells need to be undifferentiated/redifferentiated to may way for new cells to grow and differentiate?..
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Tissue culture, rooting fan leafs, and whatever else developed for use for weed is still pretty much trying to "reinvent the wheel" Because we already have many viable working cloning methods. Furthermore I think its trying to reinvent the wheel with a square because at the limits of our technology tissue culturing is a slow yet complicated long process requiring a lab. This would surely take longer than the 4 days to a week for my clippings to take root. Not even to mention the cost differance.

Now if you just must do something with the leaf then your options are making hash/oil, turn it into butter for baking or my favorite option would pack it in a box and shopping bag and leave it on the public bus.
 
cackpircings

cackpircings

Well-Known Member
I can’t believe this post is still going! About the tissue culture it is a waste of time. I know because I have given it a try. It is way too hard to keep everything sanitary. I did however get one cut node to sprout, but not long after the mold took over and killed it. It took about 10 weeks for it to even start to show the sprout. The lesson is… There is only one way to do it and that is to clone the one way that always works. It is fast and for the most part easy. I paid 55 bucks just to figure out what everyone had already told me about tissue culture. This shit is a waste of time for us homebody growers.

Keep on keepn on.
 
Syriuslydelyrius

Syriuslydelyrius

Well-Known Member
skunkyLEMon420 said:
I will believe that when me shit turns purple and smells like rainbow sherbert
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If this was directed at me I dont think you understood my post.


For everyone thats pissed off and mad at this post let me remind you that there is an "unsubscribe" button. I feel we all have the right to give opinions and comments of various posts on this topic and others and if this discussion is one you cant wait for it to die then please feel free to unsubscribe.
 
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born2killspam

Well-Known Member
I'm not trying to reinvent the wheel, just understand/experiment.. If my goal was to just duplicate plants I'd go with the tried/true like I always have..
 
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weightgain4000

Member
Alrite lets see if you can actually produce useable plants from fan leave cuttings

Here are two fan leaves under light and ends dipped in a regular root/hormone gel

I'll keep you all posted





 
KaliKitsune

KaliKitsune

Well-Known Member
I'm going to tell you right now unless those fan leaves came with part of the node you're not going to get anything but a rooted fan leaf.

Unless you've got a couple million to drop on the necessary chemicals and lab equipment to force cell deviation.
 
Syriuslydelyrius

Syriuslydelyrius

Well-Known Member
weightgain4000 said:
Alrite lets see if you can actually produce useable plants from fan leave cuttings

Here are two fan leaves under light and ends dipped in a regular root/hormone gel

I'll keep you all posted https://www.rollitup.org/%3Ca%20href=
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Scroll back a page or 2 and read some of the info I had posted earlyer where I explained a bit of the botany behind this.

I will agree with what the person above me said in that if the leaf does take root it will eventually die off and never sprout new growth.

I am just waiting for someone to do this with a leaf but doing it sneaky by leaving 1 small axillary bud site on the bottom of the leaf steam then claiming it works. This indeed could work because there is a small bit of stem/node attached to the leaf but this wouldnt be actually cloneing a leaf into a plant because a bit of the plant is still there and its that bit of stem that would be pluripotent enough to the needed cellular differentiation to grow into a new plant, a leaf all by its self just isnt capable of forming adventitious buds.

Thank you for the pictures and taking the time involved to test this, its just not going ta work though.
 
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