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Do any of you have a recommendations for general plant cell culture literature or a college/graduate level textbook? I'm looking for something that is at the same level as Freshney for mammalian cell culture but for plants. The company I work for is looking at plant cell culture as a method for...

Your instinct is right, the " buffering" in soil based substrates is not the same as normal pH buffering and I find it's WAAAYYY weaker than using a real buffer like phosphate or citric acid like people have mentioned here before. When you adjust your pH with something like a pH down or any...

IDK where you got that from but air stones are a fantastic way of getting air into water. Back in the day when I was doing mass algae production and microbial fermentation my team found that metal air stones (or diffusers) works superbly well, we were getting 120% more bio mass in our cultures...

I'm just going to give you a heads up, unless you have some gram stains looking at microbes under the microscope is super underwhelming. With out diagnostic medias you will not be able to ID who is living in your soil. Under a 40X objective they'll look just like little squiggles.

Yea I think its also worth noting that there is a difference between Organic nutrients and "organic" nutrients -The former referring to the chemistry and the latter referring to the agricultural practice. The other added benefit I can see is that some of these hydrolysates also contain some...

You are like the first person I've come across in a LONG TIME that has discuss using buffering agents in growing! I feel like it's a tool/science that everyone is missing out on. I need to nerd out for a sec but: What concentration are you using your bicarbonate at and what do you use for pH...

I've used Guar gum for some microbial inoculants and that works wonders as a natural thickening agent. you only need 1.25gr/100mL for a CloneX gel constancy

I know it seems like marketing BS from the rooting hormone companies, but the different IBA concentration have a legitimate purpose: More woody the tissue the more IBA you need to induce rooting in those cases. If you doing cannabis yea, the 0.03-0.05% stuff will work just fine but when I've...

I had that same thought, I should have been more clear about what I'd like to do and use AA's as supplements. None of the AA contain Mo, Cu, P, or K. I can only imagine that using straight AA's would cause problems with heterotrophic bacterial growth in both hydro and solid substrates. Corn...

oh shit good question....I was going to add in about 200mg/Liter AA's in addition to my nutrient solution. I feel like feeding with straight AA's would be a bad idea.

Yes you are right that callus is generated to heal a wound, the tissue it's self is essentially a clump of un-differentiated cells from around the wound site. With the cells being undifferentiated they can be come basically any plant tissue they want, and in the case of the cut clones, roots...

With those that use straight water what kind of rooting times are you getting? Like 10-12 days before transplanting?

Does anyone have experience feeding with amino acid solutions for cannabis in veg stage? I'm curious because I can see that amino acids would be a great source of carbon, nitrogen, sulfur, and oxygen (for complex molecules). They can also chelate metal ions that are critical for plants and they...

This is a question for those that root clones in EZcloner - If you do, what is the point of using low nutrient solutions in your cloner before root formation? I’ve been working with some growers recently that have told me that they as a part of their cloning process they add in products like...

Do you do Tissue culture?

You bring up a good point about how small the callus can be, I find it hard to see finer structures on the stems when the clones are in EZcloners/wet with gelled rooting substances. Do you think if I removed the clones from an EZcloner and dried them out to observe for small callus formation...

That's a great picture of callus in action, the question for me is how you induce roots from that? I did not know this was a trend in IG - I really don't go on there much. Is this guy in a hydronic system in rockwool?

Straight up: What is your opinion on the importance and the role that callus formation plays in the cloning process? Do you use callus formation as an indicator of successful clones? Do you care about callus formation? Do you induce its formation? I’ve been doing a lot of research on plant...

I actually don't think you'll ever get a lower ppm in run off than what's going in - unless you rinse your soil with RO/DI water Think of the soil as a sponge - It's going to hold on to the nutrients because they are charge particles sticking to the soil particles and when you water in more...

Yea once I get going I'm going to try some with low med and high and see what happens. maybe I'll post it in one of grow journal Threads.