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  1. O

    Marijuana Tissue Culture Success!

    have you ever used a culture rotator before. this sounds like the bees knees. not sure how this would work for the callus cultures you speak of though i will not have to use agar for cultures. as a rotator allows for a liquid medium the roots will never have dissolved oxygen issues, at least...
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    Marijuana Tissue Culture Success!

    if i were to collect the growth tips off my favorite wreckage strain and successfully sterillize them. what would you recommend i add to my media? full strength M/S? grams of sucrose per liter? what do you do for agar. i use a generic non tissue culture grade agar. for my fungal cultures. and...
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    Marijuana Tissue Culture Success!

    your not using any hormones? sweet im actually in the process of moving all my science stuff to my new house where my "lab" is located. i would rate my aseptic technique as excellent at this point, based on less that 1% of my mushroom cultures contaminating. it should be ive been doing fungal...
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    Marijuana Tissue Culture Success!

    if you wouldnt mind telling me, what is your disinfestation procedure for cannabis. i own a ultrasonic cleaner, tween 20, filter flask with hand pump vacuum(in plants from test tubes it mentions that using filter flask with a stopper and applying a 25mm HG vacuum really helps dislodge a lot of...
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    Marijuana Tissue Culture Success!

    just ordered iaa iba naa kinetin gibberilic acid all powder form plus brassinoid triacontanol fulvic acid humic acid to experiment with on ex vitro plants thinking about looking for some TZD as ive heard thats a good one according to a chinese study on hemp i already have enough MS salts to...
  6. O

    Can anyone recommend a good timer for HPA?

    does this mean you have setup, i want to see it
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    Marijuana Tissue Culture Success!

    my tent lab was designed for mushroom cultivation. i will no longer be using the tent as i now have a 12x12 room dedicated to tissue culturing and other mad scientist exploits. i only have one experiment undermy belt in plant tissue culture and that was with 20-20-20 fertillizer and vitamin...
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    Marijuana Tissue Culture Success!

    hey im glad to see your still around. i just read thru most of the thread. i have all the equipment needed to do tissue culture, however i need to order some new hormones mine are over 2 years old. heres my setup.
  9. O

    HHP's 10K HPA Build Thread!

    i was just using my rothenberger last night. it think imgoing to do the same thing only filll my red box with rocks also your rothenberger has a different hose than mine. my hose is matte and 3/4inch diameter. and yours appears shinny and skinny did you change it out?
  10. O

    Can anyone recommend a good timer for HPA?

    thats a pretty cheap timer says it goes down to 0.01 seconds. is it reliable though? nothing like spending months working on something to have everything dry up to nothing to save a few bucks
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    Can anyone recommend a good timer for HPA?

    ehco tech solenoids work pretty good you can find them on ebay heres an example, i like their stainless solenids better than their plastic, much better quality. and you can replace the electronics if they fail...
  12. O

    Can anyone recommend a good timer for HPA?

    just get this one http://www.rammeter.com/atc-automatic-timing-controls-422ar-100-s0-x-multi-range-repeat-cycle-timer.php you need this octol base as well http://www.rammeter.com/atc-automatic-timing-controls-ot-08-8-pin-din-rail-mounted-socket.php does 0.1 seconds, 20-240v AC and 12-240v DC...
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    Aquaponics - let the fish be your nutrient!

    hey dude im not sure if your raising the lights for the picture or not. but having the lights that high will cause you to lose a ton of umoles due to the walls of the tents not being to reflective
  14. O

    Might buy Atomix System from friend

    sweet are they nickel plated brass like ive heard or are they stainless steel?
  15. O

    Might buy Atomix System from friend

    whats going on with the atomix equipment pictures dude? don't leave us hanging.
  16. O

    HHP's 10K HPA Build Thread!

    AA nozzles arent really that expensive. im in the process of buying some bete flat fan nozzles. they cost $47 a piece and cover a lot of chamber each.
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    HHP's 10K HPA Build Thread!

    haha me to :)
  18. O

    HHP's 10K HPA Build Thread!

    hey hammer ive got to retract what i said. i was thinking and i did see a successful hpa grow. it was in the tricky 2011 thread. i cant remember their screen name but they grew SOG, in multiple rubbermaids and used red teffen cloudtops. he showed pictures of what he was doing and claimed he...
  19. O

    Does anybody use city water pressure to run high/low pressuer misters?

    - believe it or not i actually add chlorine to my water. 1ppm of free chlorine will actually kill all pithium in a matter of minutes
  20. O

    Might buy Atomix System from friend

    damn i posted with out looking at your reply i think im going to just steal your design instead:) whats your dimensions?
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