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  1. Wedum Boise

    Any powdery mildew experts?

    The amount of HC in cannabis may not be enough to be to lethal and like you stated it's a natural byproduct. Our bodies can consume small amounts of HC which it is also found in some common foods not just cannabis and cigarettes. Very close mind of you to only think that. However HC can become...
  2. Wedum Boise

    Any powdery mildew experts?

    This is very alarming I hope he knows he is introducing cyanide to people. 205C releases the toxic gas, lighting it up with a butane lighter will definitely exceed that temp.
  3. Wedum Boise

    Any powdery mildew experts?

    PM is an obligate biotroph, meaning it needs a host (plant) to extract nutrients from for survival. Even on an agar plate it will not grow, it needs plant material as source medium to colonize. Some pm are also host specific, a pm species that is novel on cannabis and able to germinate will not...
  4. Wedum Boise

    transporting pm in soil

    I will still be interested. We are still a month away to have a collection kit ready for shipping, so any help down the road will be very much appreciated! Let's touch base around July, early August.
  5. Wedum Boise

    PM Germination

    PM can be invisible to the naked eye by vascularizing inside the plant, and other strains can explode with PM a couple of weeks before flowering because their defenses may start to weakened prior to flowering.
  6. Wedum Boise

    PM Germination

    Does the presence of conidia (spores) an indicator that your plant is infected or is that same plant resistant to fungal development? How can you know for sure?
  7. Wedum Boise

    transporting pm in soil

    @Budley Doright would you be interested to send any PM you may still have? We are working to put together a collection where growers can isolate the conidia from the leaf and send through the mail. We need more geographic sampling to confirm single or dual species. DM for more details or...
  8. Wedum Boise

    Donate your Powdery Mildew

    Soon to be released!
  9. Wedum Boise

    Donate your Powdery Mildew

    I will need that species to grow the specific mold, hence the need for a sample and to isolate it from the plant and grow a monoculture.
  10. Wedum Boise

    Donate your Powdery Mildew

    Hi everyone, My lab is working on an assay to help detect powdery mildew early on from cannabis. We are looking to experiment on a wide variety of samples since there are many different fungi that causes it. Please inbox me if you are willing to scrape off a sample from your leaves or...
  11. Wedum Boise

    Powdery Mildew Treatment

    I found a blog that list a company in the US that has it http://greenspaceonline.info/AQ10.htm The active ingredient is a fungus but I also researched that the same species can also naturally occur in plants if there is a presence of PM. It may not be used as a preventative treatment as it...
  12. Wedum Boise

    Powdery Mildew Treatment

    Has anyone had any success using AQ10 as a treatment? and if so did it affect your yield?
  13. Wedum Boise

    sexing in perpetual

    Here is a much...much more simpler and faster way: http://www.medicinalgenomics.com/gender-detection/
  14. Wedum Boise

    Microbes comparison - Mammoth P, Recharge and Earth Juice Mycorrhizae, BioZeus, BioRighteous

    It will be more informative to also see the cfu/g to add into the comparison
  15. Wedum Boise

    Beneficial Microbes

    I've been using Tryptic Soy Broth as a media and haven't had any issues propagating the strains. You may need an incubator to provide the right temperature for optimal growth, for example Bacillus subtilis growing temp is 30C in a nutrient agar or broth. As far as disposition and handling...
  16. Wedum Boise

    Beneficial Microbes

    I use about 5mls of liquid media to reconstitute and propagate the species. I let it sit room temp for about a week until the media starts to look turbid. Fortunately we have a qPCR machine that allows to detect how much of that specific DNA is in the media sample (takes a couple of hours) and...
  17. Wedum Boise

    Beneficial Microbes

    The CSO and founder of our company Medicinal Genomics will be in Colorado next week. He would rather collect them live and transport the DNA back here. What is your email and I can introduce you two.
  18. Wedum Boise

    Beneficial Microbes

    Excellent! Let me confirm with my colleagues about the condition to send the samples in...we normally receive extracted DNA from flower through the mail but since this is plant roots it shouldn't be an issue. What state are you in?
  19. Wedum Boise

    Beneficial Microbes

    Does your grows always stay clean from a root rot in a hydro chemical set up? If you do get it how do attack it?
  20. Wedum Boise

    Beneficial Microbes

    Would you be open to send a sample of the root rot to us? We can shotgun sequence to see what species is causing that in your hydro.
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