Cytokinins (6-BAP) & Auxins (IAA)

[Guile]

Fractions of micrograms (not milligrams, I would have to be making solutions on the scale of swimming pools just to be accurate)... I can't even imagine working at those scales with the equipment I have a hold of.. Does this include the 20:1 GA to TZD solution you mentioned earlier?

This is going to sound ultra simplistic (forgive me I'm pretty stoned) but it was one of the ideas that first got me looking at hormones.
The idea was to buy some plant gel then add hormones and nutrient in the appropriate ratio so all you had do do was add water and a cutting. Assuming appropriate environmental control you would have a plant with roots in reasonable time.. I have since come to think that foliage applications might allow me more control.

That would allow you to root in a gel (similar environment to the culturing gels) while having a clean slate to apply new hormones to in the attempt to manipulate the plant in a new direction.

In regards to hormone levels wearing off, isn't this where regular foliage feeding of the plant might become beneficial?

I hope you don't take offense to all my questions and speculations, its really the best way I know to learn from someone. I may just be a "shade tree mechanic" so to speak, but I do appreciate your incite and investment in elevating my understandings..
If it makes you feel better I'll buy a share (assuming they aren't too pricy).. Do stock holders get pre-release access to the imaginary bud technology?

I think things are going to travel in some interesting directions once you get people experimenting and sharing their results.. Ofcurse when i first heard of aeronautics I figured everyone would be doing that before long, here we are quite a while latter and I'm not even doing it myself..
I see some interesting implications whenever a study describes the expression of a desirable quality in subsequent generations, I have a feeling that one day seed banks will have offerings that are the product of hormonally influenced mothers and maybe the seeds themselves will be treated before you receive them. Once upon a time people didn't think feminized seeds would take off the way they have..

By the way, you might be surprised with what can be made from bits of other stuff. Everything you have ever seen with your naked eye or laid your bare hands on, its all been recycled somehow (and you don't have to tear something down to the molecular level to re-purpose it effectively)

Getting back to my earlier experiments, I have noticed that the vegetative growth of my test plants has become less pronounced especially in the scope of internodal elongation (my plants are stretching) mind you that there have been no additional hormone treatments since test 2. this would imply to me that the GA has a longer half life than then the Cytokinins used (6-BAP) otherwise the plants are becoming more tolerant of it. Consequentially the main cola buds of my most mature test plants have become quite "airy". The top most flowers of the most mature plants from test 1 have not completely normalized retaining the fat hair quality though they are becoming longer in nature many have a rough/bumpy appearance. (i would assume this might be a product of some of the Auxins used or their concentrations)

 

[drewbot]

Does this include the 20:1 GA to TZD solution you mentioned earlier?

Sure! except don't get hung up on the numbers. Stretch can be a good thing for cuttings or shoots alone, so remember the application...

This is going to sound ultra simplistic (forgive me I'm pretty stoned) but it was one of the ideas that first got me looking at hormones.
The idea was to buy some plant gel then add hormones and nutrient in the appropriate ratio so all you had do do was add water and a cutting. Assuming appropriate environmental control you would have a plant with roots in reasonable time.. I have since come to think that foliage applications might allow me more control.

For the diffusion problem mentioned before, gel is only good because it is well studied and it holds a plant up to some degree.

In regards to hormone levels wearing off, isn't this where regular foliage feeding of the plant might become beneficial?

That's the idea

Do stock holders get pre-release access to the imaginary bud technology?

That's insider trading!

...seed banks will have offerings that are the product of hormonally influenced mothers and maybe the seeds themselves will be treated before you receive them. Once upon a time people didn't think feminized seeds would take off the way they have..

Yep that's the idea. You can program a synthetic seed to carry disease resistance. It's a short crop, so you can time release preservatives. See salicylic acid for one.

 

[Guile]

I'm about to harvest the table containing the most mature test plants so I want to collect the best data I can using what's available to me. What I was thinking about doing is using chloroformto get the most pure THC extraction (from what I understand chloroformwill dissolve less chlorophyll and plant sugars than alcohol).
This way I can compare each of the 2 treated rows with the untreated ones.

Its my understanding that I should oven dry (250 degrees for up to 2 hours) each test group before running it through a food processor (stems and all to a coarse chop)

My first question is should I then use isopropyl alcohol (30 min soak then strain) and distill off as much alcohol as possible (using a double boiler containing water) before redissolving in the chloroform, separating out the solution and distilling again?

Also Should I use a cold press to extract the solvent from the plant material, or would that just be excessive? (I have a 50 ton hydraulic press I can outfit with something like a cooking pot drilled for drainage and a pie tin to catch the run off)

How much a concern is heat? everything I have been reading seems to try and keep temperatures at or under 250F for 2 hours or less. Should I be limiting overall heat exposure or just limiting the temperatures at which its exposed? (Chloroformhas a boiling point of only 142F)

Last but not least anyone have leads on cheap glassware? I've acquired some in the past via ebay (for other projects) but over time most everything has gotten broken from improper storage. If I'm going to use chloroform I have a feeling I might need something better than an old crock pot, 4L Gallo wine bottle, and 3/8" copper coil in a home made "cold can" using rubber tubing to make the seals. Or would my crude "old school" distillation rig work sufficiently assuming the right seals are found? (Is there any particular sealing material I should use when using chloroform?)

 

[drewbot]

Trying to compare THC levels? Use thin layer chromatography. There are test kits all over the internet.

 

[Guile]

Trying to compare THC levels? Use thin layer chromatography. There are test kits all over the internet.

The thing is I want to compare total yield of THC
I'm establishing an "Overall performance" approach to comparison. Its really the most significant comparison that can be crudely made.

Don't get me wrong I am going to have to work on methods to measure other Cannabinoids as well but I planned on waiting until I began an experiment involving then directly before I crossed that bridge so to speak. However in the interest of lead research what are the strains known for producing the highest relative CBN and/or CBD concentrations?

 

[drewbot]

cbn- ruderelis varietals except for lowrider crosses
cbd- cannatonic and harlequin, followed by green crack

total yield is percent THC * dry mass. Still more accurate with the TLC unless you know how to do preferential extractions of paraffins (activated carbon chromatography). There are plenty of ways to do plant extraction. Why chloroform? What about pentane/hexane?

 

[Guile]

cbn- ruderelis varietals except for lowrider crosses
cbd- cannatonic and harlequin, followed by green crack

total yield is percent THC * dry mass. Still more accurate with the TLC unless you know how to do preferential extractions of paraffins (activated carbon chromatography). There are plenty of ways to do plant extraction. Why chloroform? What about pentane/hexane?

I'm not at all apposed to Hexane, might be interesting to have around for other experiments (one of the appealing quality's of Chloroform). It just seems to be a little more expensive per gallon. Would it make for a more pure extract? or be easier to work with?

For economy's sake I keep seeing "Colman fuel" mentioned (Naptha/white gas) though I am unclear if it needs to be distilled before hand (assumingly to remove impurity's) or the "THC extract" has to be washed (for the same reason) however I have seen internet posts that imply that neither is necessary

I plan to record the gross weight at harvest, the dry weight (after a couple hours in a 250f oven) and the total extract obtained.
I think these will give me the best overall collection of data points to make comparisons as my hormone treated plants have both more height and foliage though the buds are quite fluffy.

I fear that testing a sample bud from the tops of plants my not give a test result that is accurate to overall THC production as there might be more THC found in the foliage of one group of plants, I also find it unlikely that the lower popcorn buds will yield identical THC content per mass of bud as the upper cola. This reason alone makes measuring the plant as a single mass more appealing to me.

Additionally I am a medical marijuana care provider and I want to get my medicine to my patents.. I see many advantages to making a pure (or nearly pure) THC extract:
!, I have been dosing my plants with hormones, I would like to wash away as much of that as possible (peace of mind)
2, The laws that govern how I provide medicine count extracts gram for gram as "marijuana" allowing me alot more freedom
3, Isolating the purest form of the desired cannabinoids will make it appear more pharmaceutical in nature (lending to medical credibility)
4, Once I figure out how to isolate nearly pure CBN and CBD I will be afforded the possibility of mixing the ideal cocktail for my patents needs while at the same time cutting my ridiculous overhead (2 dozen strains currently) down to perhaps under a half dozen strains that I can more ideally accommodate rather than coming to compromises that suit all (a true compromise is where everything suffers equally).

Eventually what I would like to achieve would be to make like 3-4mm spherical pills containing a single dose of a given THC/CBD/CBN ratio that could be ingested orally (as a pill or infusion) smoked/vaporized or taken as a suppository (if desired). It would likely be the kind of thing that finally gives marijuana the medical recognition and credibility it needs (making it just like every other pharmaceutical drug that occasionally gets misused)

I'm sure at some point a real pharmaceutical company will pick up on the idea and synthesize all the most active cannabinoids to circumvent the hassles of growing and extraction but somebody has got to lead the way.. might as well make it a hick like me (that way its a slap in the face to anyone who had the resources to do it right and just chose not to).
I would love to see the pharmaceutical company's competing with the "natural extract" industry at all levels. If we lightened "drug regulations" and allowed "domestic" pharmaceutical companies to compete within all drug trade, we could put the drug cartels out of business (at least the ones operation on our soil) while creating American jobs, freeing up resources that could be focused on our real issues, and make decent national security a far more reasonable venture.

 

[Guile]

I think I figured out what the flower mutation is.. the hairs of the flowers are turning into leaves, they don't have any color yet (they are still while) however their structure is becoming distinguishable. The table containing these plants will be harvested in the next few days and I am going to try to get my help to bring a digital camera (or phone at least).

This hair/leaf mutation would be neat to recreate on a slow maturing strain (to see how far it could go).

 

[Joedank]

Forgot about this thread but for hormones and such I have changed up a little;

First month of flower : sol. Kelp 2ml per 4L h2o
Second month: 5ml alfalfa meal tea . 2ml kelp. 4Lh20
End: final by yellow bottle: the perfect organic mix of tricon and other pgr's it has helped yields alot...
Spray every four days... Sometimes adding neem or oregano oils.. And sm-90 as a sufuricant ...
Look into biobrass-16 it has crazy affects on yeilds but I can seem to buy it anywhere

 

[Guile]

Forgot about this thread but for hormones and such I have changed up a little;

First month of flower : sol. Kelp 2ml per 4L h2o
Second month: 5ml alfalfa meal tea . 2ml kelp. 4Lh20
End: final by yellow bottle: the perfect organic mix of tricon and other pgr's it has helped yields alot...
Spray every four days... Sometimes adding neem or oregano oils.. And sm-90 as a sufuricant ...
Look into biobrass-16 it has crazy affects on yeilds but I can seem to buy it anywhere

Any chance you could link me some of the products/information you reference?

I found a little on the brassinosteroid (analog) biobrass-16 (BB-16) but mostly dealing with tomatoes, rice and tropical fruit (so far anyway). How translatable are the results seen in "fruit" to that of pot? I see alot of stuff that seems to have great commercial applications when it comes to fruit, when applied to cannabis can improvements be expected in flower yield, seedless bud production, or aide in ripening?

 

[Guile]

I think I'm finally going to let this beast die... At very least the current test batch..
Though I'm harvesting the first table from it in the next couple days, my help doesn't have a camera to share for this use and I still have not ironed out a decent method to obtain my THC extract (I'm not liking the different alcohol extracts I've been trying and I haven't been able to explore petroleum based solvents). They are just going to get trimmed and dried like any other harvest (though I may segregate/label the individual rows to compare dry weights).

I think I want to give Brassinolide, Triacontanol, and N-Acetyl Thiazolidine Carboxylic Acid (NATCA) a try on the next table...

Though I think a combination ofCytokinins, Auxins, and Gibberellins could be exceedingly useful. I also think the 1:20:1 ratio using 0.5-1ppm TDZ and 2,4-D with 10-20ppm GA (maybe GA4, I've seen something that seems to indicate it might influence flowering more) would be something along the right lines. I know the GA seems low but I have recently seen it suggested at 50ppm to induce hermaphrodisum in pot plants Besides the only earthly reason for mixing this many things together is in an attempt of finding that "synergistic effect" you read about. That having been said, if you succeed a little will bit should go a long ways (right?).

My head hurts from this stuff... literally... I have no idea what ratios to use with the stuff I have on hand and there seems to be a huge public concern over most the "strictly synthetic" plant growth regulators... Atleast BAP and IAA are naturally occurring as well as Brassinolide, Triacontanol, and Gibberellins..

 

[Bonkleesha]

remember that IAA is naturally occuring, and IBA is synthetyc. i bookmarked this to finish the thread later, but i was reading u put something about switching out for another. bad idea. our plants will tolerate alot less IBA than IAA.

 

[Bonkleesha]

also, i wouldntthink much in terms of high auxins if you have healthy mothers. auxins help with advantageous formations, while the cytokynins will help with bud proliferation and things like that. i healthy mother should have plenty of its own rooting hormones. a little auxin boost would be ok, but a big blast might just be a waste. however if ur moms are jangly, ur on the right track.

 

[Guile]

also, i wouldntthink much in terms of high auxins if you have healthy mothers. auxins help with advantageous formations, while the cytokynins will help with bud proliferation and things like that. i healthy mother should have plenty of its own rooting hormones. a little auxin boost would be ok, but a big blast might just be a waste. however if ur moms are jangly, ur on the right track.

I think it was a paper I had read, something like "the dual role of Auxins in flowering" that may have provoked me to over due the auxin thing, I have read somewhere that Gibberellins encourage Male flower production where Auxins favor female. Believing that the roles of different auxins were generally confined may have also inhibited a better understanding..

I think I had read somewhere that IBA is naturally occurring in a few plants, Salix (Willow) might be one. Though it does seem to be most closely associated with root development (which does seem unnecessary for a healthy established plant)

Without any exact figures I can say at this point the Wappa exposed to only the second test yielded by far more "wet" than anything else on the table. This could be in part to its more centralized location on the table (receiving amongst the highest light concentrations of the table). However they did out preform the next row back (sharing similar light conditions).

The buds of the heaviest plants were very airy and hard to manicure having many bud leaves that were large enough to warrant full removal (rather than just tip trimming..

I believe I can now better appreciate the perspective shared earlier about focusing on "stress inhibitors" and want to compare results before reintroducing other hormones in future experiments (it will give me a chance to better understand the general landscape).

I think formulations like those we have been discussing here would most likely be more beneficial during early development/vegetation.. It seems these hormones are most productive when focused on a particular stage of development which may not particularly be "flowering" Though the role of Auxins/Gibberellins in flowering may work in the direction I would like, Its clear I need to better understand more specifically how they work first.

The anti stress hormones should be an experience in its own right as well.. I have a particular interest in Triacontanol which seems as though it might be difficult to keep in suspension (I read somewhere that "Canna Boost" might be an easy to work with Triacontanol product).

Does anyone know if any Brassinolide or NATCA products on the market?

 

[thedude27]

Yes Brassinolide is(or was) on the market as I have some. I also have GA3, IBA, Benzylaminopurine, IAA, Triacontanol, and NAA. I think thats all of them.

I used to get all this stuff from supergrow.biz or something like that, last I heard the person that owned it went on sabbatical and I havent seen the site anymore. However, normal human beings can obtain these substances so look around I'm sure someone is selling it somewhere.

I have a bunch of information off their website that I saved related to dosing and performance on less controversial plants. I'll dump some of it here (I apologize about the formatting in advnace) for you.

 

[thedude27]

Gibberellic acid is an important plant hormone used to improve seed germination and plant growth and size. Gibberellic acid can also influence the timing of flowering, flower gender and flower size.

User Feedback
Citrus

I sprayed GA3 (35ppm) on my citrus trees last week when they were starting to bloom. I checked them yesterday and they are all loaded with blooms. G.H. User Group March 31, 2005 9:24 PM
Lawn - GA3

1. I used a soil mix of 8 parts top soil, 2 cow manure, .5 peat, and .25 Perlite(Krum) @ 2-4 inches deep.
2. Sprayed lightly with a hose before covering the sewn grass seed.
3. I used stems from last years flowers that were like strong hay, criss-crossed them over the seeded moist soil mix, and then piled oak leaves 1-2 inches deep over that to create heat for the grass.
4. On late March early April near Chicago the 6th day of growth, I pulled off the cover and sprayed the grass in 3 diff locations full sun, part sun, and shady. I put the cover back on after spraying and 1-2 days later gave it a spritz of water. I sprayed GA3 3 days after that and then 2 days after that.
5. There was no presoak.
6. The temperature was approx. 50-70 F during the day and no lower than 45 F at night. 2 of the days it was 78-82 F.
So, from the 6th day of growing to 1.5 weeks of spraying 3 times later I have some good grass. The shady grass seemed to grow the quickest. It might have been because of a less variant temp. I tried the same areas last year without covering the seed, without special potting soil, and without spray GA3.
Super-Grow note: the gibberellic acid concentration was 35 PPM for each of the three sprayings.
User Group
Lilac - GA3

My new very small hybrid French lilacs are doing well as a result of regular spraying with roughly 50 ppm GA3 solution.
John M., User Group - June 2, 2005
Lilac - GA3 & Fulvic Acid

I have 3 lilac bushes. One is over 6 years, one is 5 years, and one is 2 years. The 5 year plant wouldn't bloom till I sprayed GA3 35-70 PPM 4 times along with 2x85 PPM of fulvic acid.
The 6 year one gave us only a couple blooms last year. This year after spraying GA3/Fulvic we have 30-50 blooms and the plant is about 15 feet tall.
J.T., User Group - June 2, 2005
Peppers, tomatoes - GA3

I tried a 70ppm solution of GA3 on 5 of my habanero pepper plants and on 5 each of my big boy and cherry tomato plants. After 3 weeks I couldn't see any difference between them and the other plants of the same type. I then used a 140ppm solution of GA3 on the same plants and now, 1 week later I can tell a major difference in the height of the treated plants as compared to the untreated ones and also the treated plants are beginning to bloom.
A.L., User Group - May 28, 2005
Rasberries - GA3

I have a patch, here in RI, water is very important too. If you are trying for a sudden boost use over 500ppm. If you want sustained growth improvement 50ppm is good.
Willy Wonka, User Group - May 5, 2005
Rasberries - GA3 & Fulvic Acid

I had some great results by spraying Raspberries also this year. We have 42 new shoots about 3-4 feet high after spraying 85 PPM Fulvic 2 times and GA3 35-70 PPM 5 times. We should have berries soon! We had enough berries last year to make 2 huge pies. I suspect we with get 5-6 pies this year.
J.T., User Group - June 6, 2005
Squash - GA3 & GrowTonic

My "Early White Bush Scallop" squash plants are massive! They were given GA3 at 300ppm 22 days after emerging and GrowTonic at 36 days.
User Group, July 17, 2005 7:50 PM
Tomatoes - GA3

I used GA3 at 150 PPM on my tomato plants 36 days after transplanting. That was June 28th. When I applied it I had only 2 "Early Girl" tomatoes the size of a pea showing. Quite a few flowers were about to open on both "Early Girls" and "German Johnsons" when I applied it. Here we are at 13 days after spraying and I have 75 tomatoes growing now!!!
Every plant that I've used GA3 on has responded very well. I've seen many gardens around here and no ones plants are bigger or stronger looking. [D.Z. West Virginia - July 11, 2005 - http://groups.yahoo.com/group/gibberellic_acid/message/1351]
I noticed a big difference in my tomato plants after spraying them with a 200ppm of GA3. [D.S. - User Group January 8, 2005]
Miscellaneous

... things I've noticed about GA3 are that in general plants emerge faster from winter thaw to luscious greenness. I have 6 climbing flowering vines that greened up 2 weeks earlier than last year. The established tulips came up with multi-headed flowers that are extremely huge around. The new honeysuckle bulbs died right on queue, but the 3 year old honeysuckle is still blooming strong. I also was able to induce the troublesome Lilac to bloom this year.

Frequently Asked Questions
Introduction to Gibberellic Acid

1. What is Gibberellic Acid (GA3)?
2. Is Gibberellic Acid An Organic Product?
3. How Does Gibberellic Acid (GA3) Help Plants?
Benefits of Using Gibberellic Acid

4. How Do I Use Gibberellic Acid (GA3) To Improve Seed Germination?
5. Does Seed Chilling In Distilled Water Also Affect Seed Germination?
6. What Is Seed Scaring And What Does It have To Do With Gibberellic Acid (GA3)?
7. Can I Use Gibberellic Acid During Vegetating And Flowering?
8. How Do I Use Gibberellic Acid (GA3) To Generate Bigger Plants?
9. Can Gibberellic Acid (GA3) Induce Plants To Flower?
10. Can Gibberellic Acid Induce Bigger Flowers?
11. Can Gibberellic Acid (GA3) Induce More Flowers?
12. What Is The Use Of Gibberellic Acid In Producing Bigger Yields?
13. How Do I Use Gibberellic Acid (GA3) To Control The Gender Of The Flowers?
14. Can I combine Gibberellic Acid (GA3) With Other Plant Hormones?
15. How Does Gibberellic Acid Affect Roots?
Using Gibberellic Acid

16. How Do I Use The Powdered Gibberellic Acid (GA3)?
17. How Should I Dilute The Gibberellic Acid (GA3) Powder?
18. What Should I Use To Dilute The Gibberellic Acid (GA3) Powder?
19. How Much Alcohol Should I Use?
20. Doesn't Dissolving Powder Gibberellic Acid (GA3) With Alcohol Damage Plants?
21. Can Powdered Gibberellic Acid (GA3) Be Diluted In Water?
22. How Do I Use The Gibberellic Acid (GA3) Tablet?
21. Does It Make A Difference If I Water With Gibberellic Acid (GA3) Instead Of Misting?
22. Can I Mix Gibberellic Acid With Brassinolide To Use As A Foliar Spray?
23. How Do I Use the Gibberellic Acid Paste?
Buying Gibberellic Acid

24. In What Form Is Gibberellic Acid (GA3) Commercially Available?
25. Which Type Of Gibberellic Acid (GA3) Is The Best One To buy?
26. Should The Gibberellic Acid Powder Be Above a Particular Purity To Be Effective?
27. How Much Gibberellic Acid (GA3) Would I need?
Storing Gibberellic Acid

28. How Should I Store Gibberellic Acid (GA3) Powder?
29. How should I Store Liquid Gibberellic Acid (GA3)?
30. What Precautions Should I Use In Handling Gibberellic Acid (GA3)?
31. How is Gibberellic Acid (GA3) Produced?
32. What Is The Difference Between Gibberellic Acid And GA3?
33. Is There A User Group For Gibberellic Acid (GA3)?
34. Can I Set Up a Link To This Page?
Plant Specific Uses of Gibberellic Acid

35. What Are The Effects Of Gibberellic Acid On Palm Tree Germination?
36. What Are The Effects Of Applying Gibberellic Acid On Tomato Plants Before Transplanting?
37. What Are The Effects Of Applying Gibberellic Acid On Tomato Plants When Transplanting?

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1. What is Gibberellic Acid (GA3)?
Gibberellic acid (GA3) is a naturally occurring plant hormone that regulates the growth of plants, including triggering seed germination. There are over 100 different Gibberellic acids, and plants will have several different types. Rice has fourteen different gibberellic acids while corn has twelve different Gibberellic acids.

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2. Is Gibberellic Acid An Organic Product?
OMRI, the leading organic certifying organization has certified many Gibberellic acid products.

http://www.dirtworks.net/Images/omri list.pdf

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3. How Does Gibberellic Acid (GA3) Help Plants?
The main areas of activity are to improve seed germination, bigger plants and flowers and manipulating the gender of flowers. There are also some reports that it will also generate a larger number of female plants. This aspect is generally undocumented and requires research. Depending on how you use gibberellic acid (GA3), you can generate male flowers on a female plant and use the pollen from the same plant to generate female clones of itself or to pollinate another female plant.

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4. How Do I Use Gibberellic Acid (GA3) To Improve Seed Germination?
Gibberellic Acid will improve seed germination. The generally used method is to prepare a gibberellic acid (GA3) liquid solution and to soak the seeds in it for 24 hours [SUP]1[/SUP]; the GA3 concentration should be in the range of 100-250 PPM [SUP]1[/SUP].
One manufacturer [SUP]2[/SUP] of gibberellic acid products recommends first trying a concentration of 50 PPM.
One website [SUP]3[/SUP] recommends using 500 PPM for rosulate Violas, which seems rather high.
Older Seeds

One botanist used Gibberellic Acid when successfully germinating 2,000 year old seeds from an extinct plant [SUP]4[/SUP].
In some cases the seedling may grow so quickly that it begins to lean over. If this happens add more soil around the base of plant.

1. Gibberelic Acid [http://www.flytrap.demon.co.uk/cc/data/ga3.htm]
2. http://www.megagro.com/megagro_faq.htm
3. http://freespace.virgin.net/almond.jim/alpseed.htm#Gibberellic acid
4. http://sfgate.com/cgi-bin/article.cgi?f=/c/a/2005/06/12/MNGJND7G5T1.DTL

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For more information on improved seed germination please take a look at Brassinolide, Chitosan, Fulvic Acid and Gibberellic Acid.
Order the Super-Grow Germination Pack!

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5. Does Seed Chilling In Distilled Water Also Affect Seed Germination?
Yes; this is known as cold stratification. In a normal life cycle seeds will spend some amount of time dormant over winter. Research [SUP]1[/SUP] studies have shown that this can be critical in improving seed germination. The same research suggested a six week chilling period.

1. Effect of scarification, GA and chilling on the germination of goldenrain-tree (Koelreuteria paniculata Laxm.) seeds [http://ipe.ibrc.unesp.br/ftp/PDFS/koelreuteria.pdf]

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6. What Is Seed Scaring And What Does It have To Do With Gibberellic Acid (GA3)?
Some seeds have an outer shell that is hard and nearly water-proof. Since seed germination means the seed has to absord the gibberellic acid solution this is an obvious problem. The objective of seed scaring is to damage the seed's outer-shell to allow water to enter the seed. Different scaring methods are used [SUP](1)[/SUP].
(1) Seed Scarification [www.mcdb.ucla.edu/Research/Hirsch/ss.html]

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7. Can I Use Gibberellic Acid During Vegetating And Flowering?
Gibberellic acid is best used during germination, vegetating and to induce flowering, but not during flowering. When flowering a plant is supposed to use all its energy to generate flowers, and its growth is normally slowed down. If gibberellic acid is used during flowering the plant will grow more but have less resource for generating flowers.

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8. How Do I Use Gibberellic Acid (GA3) To Generate Bigger Plants?
Yes. This is a well-researched area, although the results of gibberellic acid (GA3) applications vary depending on many factors, including (here also) the type of plants its applied to. In one study of persimmon yield [SUP](1)[/SUP] it was found that applications of 15 to 30 PPM increased yields by 50% to 400%. In another study [SUP](2)[/SUP] it was even found that if gibberellic acid is applied to a plant the next generation of the plant would also benefit from faster flowering and increased height. In another study of walnut trees it was found that applications of gibbarellic acid (GA3) increased growth by 567% [SUP](3)[/SUP].
1) Increasing Persimmon Yields With Gibberellic Acid [www.actahort.org/books/120/120_32.htm]
2) Generations Living with Gibberellic Acid [www.sidwell.edu/us/science/vlb5/Independent_Research_Projects/cgraham/]
3) Gibberellic Acid for Fruit Set and Seed Germination [www.crfg.org/tidbits/gibberellic.html]

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9. Can Gibberellic Acid (GA3) Induce Plants To Flower?
One researcher [SUP]1[/SUP] concluded that flowering can be induced in spathiphyllum by gibberellic acid (GA3) at concentrations of 500 PPM or less, while the best range is probably between 200 to 400 PPM. It is recommended to first start with a few plants to test the best response. Another report [SUP]2[/SUP] concludes that early flowering can occur at concentrations between 50 to 200 PPM. One study [SUP]3[/SUP] of cyclamens accelerated flowering by 4 to 5 weeks with one foliar spray of 50ppm 60 to 75 days before the plant would normally flower.
Camellia
The use of gibberellic acid to induce flowering of camellias is considered to be a common practice. While not all varieties will respond the same, the gibberellic acid treatement should generate some blooms within 40 to 45 days according to one site [SUP]4[/SUP]; another site has it at 30 to 90 days [SUP]5[/SUP]. The process can even generate bigger flowers [SUP]6[/SUP]. One method [SUP]7[/SUP] is to place one drop of the solution on the cup.

1. Inducing Flowering of Spathiphyllum with Gibberellic Acid (GA3) [mrec.ifas.ufl.edu/FoliageDigest/v4n3.html]
2. Gibberellic Acid for Fruit Set and Seed Germination [www.crfg.org/tidbits/gibberellic.html]
3. Gibberellins - Plant Growth Hormones [www.hydroponics.com.au/back_issues/issue11.html]
4. Gibbing Camellias [www.camellias-acs.com/culture/gibbing.html]
5. http://members.cox.net/vacs/gibbing.htm
6. http://members.cox.net/vacs/gibbing.htm
7. www.hgtv.com/hgtv/gl_trees_shrubs_flowering/article/0,1785,HGTV_3646_1399133,00.html

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For more information on improved flowering please take a look at BenzylAminopurine, Gibberellic Acid and Naphthalene Acetic Acid

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10. Can Gibberellic Acid Induce Bigger Flowers?
Yes! One study [SUP]1[/SUP] concluded that gibberellic acid (GA3) can also generate bigger flowers that are 25% to 50% larger when Gibberellic Acid is applied at 5 PPM directly to the flowers of gardenias or geraniums. This type of result would be applicable to most flowering plants.

BenzylAminopurine will also produce bigger flowers.

1. Gibberellins: Plant Growth Hormones [www.hydroponics.com.au/back_issues/issue11.html]

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11. Can Gibberellic Acid (GA3) Induce More Flowers?
Yes! One study [SUP]1[/SUP] conducted on the lily garden plant found a 243% increase in the number of flowers at 25 ppm.

1. Comparative Effects Of Promalin And GA3 [www.actahort.org/books/292/292_23.htm]

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12. What Is The Use Of Gibberellic Acid In Producing Bigger Yields?
A study on persimmons [SUP]1[/SUP] increased yield by at least 50%. This was done with a foliar spray of 15 to 30 ppm when the plants where at full bloom.

http://www.actahort.org/books/120/120_32.htm

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13. How Do I Use Gibberellic Acid (GA3) To Control The Gender Of The Flowers?
This area has less research than seed germination, but some information is available and even. Agriculture Canada has this as an effective procedure [SUP]4[/SUP]. One website [SUP]1[/SUP] has described that to induce male flowers a spray of 10 to 200 PPM; female flowers are induced with 200 to 300 PPM and more than 600 PPM will hinder any flowering.
Medical Marijuana

For various reasons there has been some significant interest in affecting the gender of cannabis plants. It' been reported [SUP]3[/SUP] that in 1978 Galoch published a procedure for generating male flowers on a female plant. For five consecutive days spray a plant with a 100 ppm gibberellic acid solution. Within two weeks male flowers may appear. There is much information on the internet about this use of gibberellic acid (GA3), but it does require some level of trial and error before being a reliable process. Also, the results will likely vary depending on which type of plant you are using it on.

1. Inducing Flowering of Spathiphyllum with Gibberellic Acid (GA3) [mrec.ifas.ufl.edu/FoliageDigest/v4n3.html]
2. Gibberellic Acid for Fruit Set and Seed Germination [www.crfg.org/tidbits/gibberellic.html]
3. Marijuana Botany [www.mellowgold.com/grow/mjbotany-removed/marijuanabotany3.html]
4. http://res2.agr.gc.ca/harrow/publications/pdf_pubs/p1902_e.pdf

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14. Can I combine Gibberellic Acid (GA3) With Other Plant Hormones?
Super-Grow has created GrowTonic by combining Gibberellic Acid with other hormones (Indole Butyric Acid and Naphthalene Acetic Acid) that induce root development. The combination of hormones will boost growth in different parts of the plant so they work together quite well.
There is existing research [SUP]1[/SUP] indicating that combining Gibberellic Acid and Indoleacetic Acid will improve plant growth.

1. http://www.usc.edu/CSSF/Current/Projects/S1314.pdf

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15. How Does Gibberellic Acid Affect Roots?
There has been a very interesting observation [SUP]1[/SUP] made about the effects that Gibberellic Acid has on roots. When it is used with auxins and in the dark it helps develop roots while when its light it slows down the development of roots. Given this information and other opinions [SUP]2[/SUP] it would be best to avoid Gibberellic Acid when trying to boost root development. But Gibberellic Acid is still a great tool when trying to boost other plant areas.

1. http://www.botany.org/ajb/00029122_di001682.php
2. http://www.crfg.org/tidbits/gibberellic.html

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16. How Do I Use The Powdered Gibberellic Acid (GA3)?
Powder gibberellic acid (GA3) is a white powder ranging from water-soluble 20% to alcohol-soluble 90% pure. Before it can be used is must be turned into a liquid.

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17. How Should I Dilute The Gibberellic Acid (GA3) Powder?
The amount of gibberellic acid (GA3) that needs to be used is very small. If you're feeling very scientific you can use a test tube, but we use one of those small plastic jars that prescription pills come in. Drop the correct amount of powder in it, then add a few drops of methyl hydrate. Methyl hydrate is easy to find and inexpensive; we visited three hardware stores and found it in all three for less than $3.00 for enough to last years of use. To add a drop at a time you can use an eyedropper. We went to the pharmacy and bought a bunch of syringes (less the needle) for $0.10 each. Before mixing the GA3 practice dropping single drops with one of the syringes. Once the GA3 is liquefied with the methyl hydrate add water. We have an illustrated guide at MixingGA3.jsp, and a Parts-Per-Million (PPM) mixing calculator.

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18. What Should I Use To Dilute The Gibberellic Acid (GA3) Powder?
We prefer to use Methyl Hydrate (99.9% pure), generally available in hardware stores. We have also tested dilution with 70% isopropyl alcohol (rubbing alcohol) with good results. If you are using isopropyl alcohol don't go below 70%.

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19. How Much Alcohol Should I Use?
The only reason to use alcohol is to dilute the gibberellic acid (GA3) powder. Use just enough alcohol to wet the gibberellic acid (GA3) powder. If after a couple of minutes you can still see some powder add a few more drops of alcohol.

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20. Doesn't Dissolving Powder Gibberellic Acid (GA3) With Alcohol Damage Plants?
No! It is often being said that alcohol will damage plants. Yes, but not in the very low concentration needed to dissolve gibberellic acid (GA3). In fact, the use of alcohol is encouraged [SUP]1[/SUP] in seed germination as a fungicide, where it is used in much higher concentrations - as much as 5% - than for disolving Gibberellic acid (GA3).

1. The Effects Of Fungicides Upon The Germination Of Corn [www.oznet.ksu.edu/historicpublications/Pubs/SB041.PDF]

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21. Can Powdered Gibberellic Acid (GA3) Be Diluted In Water?
It depends on which powder you are using. Super-Grow carries a 90% GA3 powder that will not dissolve in water and a 20% powder that will.

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22. How Do I Use The Gibberellic Acid (GA3) Tablet?
Gibberellic Acid (GA3) tablets are a lower concentration than powder, which is usually between 85% and 95% while tablets are between 10% and 20%. The reason is that the tablets have been treated to be water soluble! Just drop the tablet in the water, instead of the more complex use of gibberellic acid (GA3) powder that requires different dilution.

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21. Does It Make A Difference If I Water With Gibberellic Acid (GA3) Instead Of Misting?
I really haven't seen any scientific data about this, but keep in mind that liquid gibberellic acid (GA3) loses potency very quickly, so if it's in the soil through watering I would expect that it would mostly be wasted. If it's misted on the leaves it dries up and is absorbed much more quickly. Also, you would likely require a lot more gibberellic acid (GA3) for watering than for spraying/misting.

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22. Can I Mix Gibberellic Acid With Brassinolide To Use As A Foliar Spray?
Yes. Both gibberellic acid (GA3) and brassinolide are naturally present in plants so combining them as a spray is a great way to get even better results.

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23. How Do I Make and Use the Gibberellic Acid Paste?
Gibberellic acid paste can be made without much trouble. In one study [SUP]1[/SUP] paste was applied in a band around the terminal bud of trees three times in one summer. The treated trees grew an 8.5 ft. and only 1.5 ft. for the untreated trees.

1. http://www.crfg.org/tidbits/gibberellic.html

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24. In What Form Is Gibberellic Acid (GA3) Commercially Available?
Gibberellic acid (GA3) is available as a liquid, powder or in tablet form.

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25. Which Type Of Gibberellic Acid (GA3) Is The Best One To buy?
Gibberellic acid (GA3) can only be used as a liquid. But once gibberellic acid (GA3) is a liquid it's only effective for a few weeks, even if refrigerated [SUP](1)[/SUP]. So when you buy gibberellic acid (GA3) only buy it as a powder or tablet since it's likely that the solution was mixed more than a few weeks ago.
(1) Gibberelic Acid [http://www.flytrap.demon.co.uk/cc/data/ga3.htm]

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26. Should The Gibberellic Acid Powder Be Above a Particular Purity To Be Effective?
No. While powder gibberellic acid (GA3) is generally between 85% to 95% pure they all work just as well.

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27. How Much Gibberellic Acid (GA3) Would I need?
Very little! Typically, when germinating ten seeds we will use 0.02 grams of 90% gibberellic acid (GA3) powder to make 60 ML of 300 PPM gibberellic acid (GA3) soaking solution.

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28. How Should I Store Gibberellic Acid (GA3) Powder?
Store gibberellic acid (GA3) In a cool dry place in a closed container. Gibberellic acid (GA3) should not be exposed to temperatures above 40°C [SUP](1)[/SUP].
(1) Material Safety Data Sheet [www.noracconcepts.com/norac%5CNorSite.nsf/WebMSDS/Activol?OpenDocument]

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29. How should I Store Liquid Gibberellic Acid (GA3)?
While it's recommended to only mix enough gibberellic acid (GA3) to use right away, it's not always possible. While there are claims that liquid gibberellic acid (GA3) can keep for years that claim is suspect unless the liquid is frozen. A good method is to put the liquid in an ice cube tray so that you can thaw an ice cube when needed. PLEASE, label the ice cube tray!

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30. What Precautions Should I Use In Handling Gibberellic Acid (GA3)?
Use normal precautions, being that you should wash your hands after using it and avoid swallowing or breathing it in. Keep in mind that the use of gibberellic acid (GA3) is recommended for helping the production of various fruits and vegetables [SUP](1)[/SUP].
(1) The use of Gibberellic Acid to improve post-harvest handling and storage quality of cherries [www.hortnet.co.nz/publications/science/gatrials.htm]

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31. How is Gibberellic Acid (GA3) Produced?
Gibberellic Acid (GA3) is not manufactured; it's a natural product extracted from the Gibberella fujikuroi fungus. Gibberellic Acid (GA3) is already naturally found in plants and using gibberellic acid (GA3) will help you get better results from your plants.

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32. What Is The Difference Between Gibberellic Acid And GA3?
Gibberellic acid (GA3) is one of the known forms of gibberellins. There are over 100 known forms of gibberellic acid; GA3 is the most effective, with GA4 and GA7 also occasionally being used but less effective.

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33. Is There A User Group For Gibberellic Acid (GA3)?
Yes, there is a gibberellic acid (GA3) user group. You can get more information at Ga3ug.jsp.

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34. Can I Set Up a Link To This Page?
Yes, just copy this code to your web page:
<A href="http://www.super-grow.biz/GA3FAQ.jsp">The Gibberelic Acid (GA3) FAQ</A>

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35. What Are The Effects Of Gibberellic Acid On Palm Tree Germination?
Improved germination has been reported by soaking palm seed in a gibberellic acid solution of 10 to 2000 parts-per-million (PPM) for 1 to 3 days. Because gibberellic acid may cause elongation problems it would be recommended to only use the lower concentration of 10 PPM for only 1 day. If any elongation does occur Benzylaminopurine will correct the problem.

IMPORTANT: it is best to try germinating the palm seeds naturally and only use the giberellic acid if that doesn't work.

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36. What Are The Effects Of Applying Gibberellic Acid On Tomato Plants Before Transplanting?
One study [SUP]1[/SUP] concluded that 10 days before transplanting tomatoes should be supplied with 60 PPM of gibberellic acid.

1. www.ansinet.org/fulltext/jbs/jbs16448-450.pdf

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37. What Are The Effects Of Applying Gibberellic Acid On Tomato Plants When Transplanting?
One study [SUP]1[/SUP] concluded that a spray of 50 PPM of gibberellic acid when the tomato plant was transplanted outdoors increased yield by 40%.

 

[thedude27]

My personal experience is < 100ppm foliar for GA3 not more than 2 times is a reasonable dose that makes less "stupid" stuff happen.

 

[thedude27]

Benzylaminopurine is an important cytokinin that stimulates a wide array of plant growth benefits: cell division, more side branches, basal shoot formation, bigger flowers, and fruit set. 6-Benzylaminopurine also inhibits senescence.

[h=3]Dissolving Benzylaminopurine[/h]The textbooks indicate that NAOH is required to dissolve benzylaminopurine. But NAOH is not easy to prepare. Fortunately, a member of our user group has come up with an easier and better way to dissolve benzylaminopurine. Basically, we placed 1 scoop of benzylaminopurine in a small glass container with a small amount of 70% store-bought rubbing alcohol and a few drops of no-tear baby shampoo. This was kept heated between 110 and 150 Fahrenheit for a few minutes and the benzylaminopurine fully dissolved.
[h=3]Diluting Benzylaminopurine[/h]Once the Benzylaminopurine has been dissolved (see above) you mix it in water to dilute it. How much water to use depends on what Parts-Per-Million (PPM) you will use. To decide on the best PPM please take a look at the research and information that is available. The best place to start would be the FAQ on this page.
Once your know what PPM your want look at the "Benzylaminopurine Mixing" table below to decide how much water to use. The table indicates how many scoops of powder to use with how much water. After dissolving add it to the water, do not add the water to it.
[h=3]Using The Solution[/h]The 0.47 liter was put in a spray bottle and the plant was sprayed until the solution was dripping from the plant. As a safety measure it is always a good idea to spray the plant outside if possible or in a closed separate room.

Frequently Asked Questions
1. What Is Benzylaminopurine?
2. What Are The Effects of Benzylaminopurine?
3. How Does 6-Benzylaminopurine Affect Apical Dominance And The Number of Branches?
4. What Treatment Frequency Has Been Tested?
5. How Is Benzylaminopurine Used to Generate Bigger Flowers?
6. What Effect Does Benzylaminopurine Have On Plant Senescense (Death)?

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1. What Is Benzylaminopurine?
Benzylaminopurine is a cytokinin that has a large array of effects. It speeds up cell growth and division, buds, flowering and branching.

www.agrocare.com.cn/Products/6-benzylaminopurine.htm

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2. What Are The Effects of Benzylaminopurine?
Benzylaminopurine has been found [SUP]1[/SUP] to increase the thickness of stems, increase the leaf surface and the number of side branches. At the same time root growth was slowed down. This is very likely because the larger leaves provide more nutrients to the plant and reduce the need for root mass. At higher concentrations of 300 to 400ppm stem elongation was reduced.

1. http://altair.chonnam.ac.kr/~horti/vegeta/reseMan/chungsj/re1.htm

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3. How Does 6-Benzylaminopurine Affect Apical Dominance And The Number of Branches?
Tests on different plant types have shown that Benzylaminopurine will increase the number of branches. The Ontario Hosta Society reports [SUP]1[/SUP] that wetting a hosta-leaf with 1000 PPM to 3000 PPM will cause the plant to branch out simmilar to cutting the end of the branch without the damage that pruning would cause. A Plumeria Society Research Bulletin [SUP]2[/SUP] also reports that if a branch is pruned 6-Benzylaminopurine will generate more branches than prunning alone.

In another test [SUP]3[/SUP] on Anthurium 'Ozake Red' plants ethephon, PBA and BA showed that the best results were 1000 PPM 6-Benzylaminopurine.

Another test [SUP]3[/SUP] was conducted on Dieffenbachia with foliar applications of 500, 1000 and 2000 PPM of Benzylaminopurine. All treated plants averaged 6 lateral shoots while untreated plants at 2. At the same time plant height was unaffected by the Benzylaminopurine.

1. www.rittenhouse.ca/hortmag/Hosta/Hosta-Spring00.htm
2. www.theplumeriasociety.org/prb/prb_1-2.html
3. http://mrec.ifas.ufl.edu/Foliage/Resrpts/rh_90_12.htm

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4. What Treatment Frequency Has Been Tested?
Tests on a nonbranching Dieffenbachia hybrid indicated [SUP]1[/SUP] that a foliar spray for three days in a row had more lateral shoot development than plants treated only once or even on two consecutive days. At the same time plants treated with 500 or 750 PPM had better results than 250 PPM. The new shoots were visible 4 weeks after treatment.

1. http://mrec.ifas.ufl.edu/Foliage/Resrpts/rh_90_12.htm

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5. How Is Benzylaminopurine Used to Generate Bigger Flowers?
Research [SUP](1)[/SUP] on chrysanthemum indicates that benzylaminopurine can influence flower size. In this study 0.1 to 1.0 mg/l improved flowering while 10 mg/l inhibited flowering. Please not that the dosage in this experiment is surprisingly small and may only be due either to the specific type of plants or some other unknown factor.

One Benzylaminopurine medicinal marijuana user has reported that he used the BA6 at 300 PPM 4 weeks into bloom and the results were amazing, with the flowers being noticeably bigger on the sprayed plants than the unsprayed plants 1 weeks after application. He tried it at 150 PPM but 300 PPM being better. Another medicinal grower has tried to replicate these results but did not report much difference. Keep in mind that the difference in results can again be attributed to other factors such as the genetics of the plants. Another possibility could be that one of the individuals may have measured wrong. This said I believe that using benzylaminopurine to improve flowering is effective but I would suggest using lower dosages than 150 PPM.

Gibberellic Acid will also improve flower size.

1. www.jstage.jst.go.jp/article/jpestics/29/4/29_308/_article

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For more information on improved flowering please take a look at BenzylAminopurine, Gibberellic Acid and Naphthalene Acetic Acid

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6. What Effect Does Benzylaminopurine Have On Plant Senescense (Death)?
One study [SUP]1[/SUP] found that an application of 100 PPM of Benzylaminopurine was most effective in delaying plant senescense. The study concluded that plant tisue death was reduced by about half.

 

[thedude27]

Brassinolide improves plant growth through improved root growth, better seed germination, plant photosynthesis, resistance to cold, water shortage and disease.

Instructions
Brassinolide 0.1 is soluble in 70% rubbing alcohol that has been preheated to 50-60 centigrade or 125-140 Fahrenheit.
Instructions
The easiest and safest way to do this is to heat up a pot of water. Then remove the pot from the stove and put a small container in the water with the brassinolide and a small amount of alcohol. Once it has dissolved add it to the water, do not add the water to the brassinolide/alcohol.
To use Brassinolide 0.1 you can either foliar spray the plant or water the plant with it after diluting it.
Please read the FAQ and select the PPM that seems the best for you type of plant and the type of results you want. It may be smart to start at an even lower PPM and over time, depending on results, you can raise or lower the PPM.

Frequently Asked Questions
[h=3]General Brassinolide Information[/h]1. What Is Brassinolide?
2. When Was Brassinolide Discovered?
3. Why Is Brassinolide Only Available In Such Low Concentration?
[h=3]Brassinolide Effects[/h]4. How Does Brassinolide Help Plant Growth?
5. Does Brassinolide Help Plant Use Photosynthesis?
6. Does Brassinolide Help Plants Resists Cold, Drought And Salinity?
7. Does Brassinolide Help Plants Resist Disease?
8. Can Brassinolide Help Plants Deal With Flooding?
9. What Effect Does Brassinolide Have On Root Development?
10. Does Brassinolide Affect Seed Germination?
[h=3]Using Brassinolide[/h]11. What Concentrations Of Brassinolide Are Used?
12. When Should Brassinolide Be Applied?
[h=3]Storing Brassinolide[/h]13. How Do I Store Brassinolide Powder?
14. How Do I Store Brassinolide After Mixing It?
15. How Long Is Brassinolide Powder Good For?

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1. What Is Brassinolide?
Brassinolide is a naturally occuring plant steroid; it is normally found in plants [SUP]1[/SUP]. In fact, it was first discovered in plants. Brassinolide has been found to be an important element for plant growth [SUP]2[/SUP].

1. http://journal.kcsnet.or.kr/publi/bul/bu02n10/1473.pdf
2. www.botany.utoronto.ca/courses/BOT421H/Papers/microarray 2.pdf

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2. When Was Brassinolide Discovered?
In 1968 it was reported that a plant extract improved plant growth.[SUP]1[/SUP]
1. http://www.chm.bris.ac.uk/motm/brassinolide/brassinolidej.htm

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3. Why Is Brassinolide Only Available In Such Low Concentration?
Many products are available as 90% pure while we sell Brassinolide in less than 1% pure. The problem is that it is very expensive to make Brassinolide more than 1% pure and is not economical for most use.

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4. How Does Brassinolide Help Plant Growth?
Brassinolide is essential for plant growth; plants that cannot generate their own brassinolide will become dwarf plants [SUP](1)[/SUP]. In fact, it has been suggested [SUP](2)[/SUP] that the more brassinolide is available to the plant the more it will grow.
(1) Carnegie Institute [http://carnegieinstitution.org/Yearbook_HTML_00_01/dpb.html]
(2) Genetically Altering the Appearance of Crops [www.angelfire.com/oh/geneticsgroup7/heather.html]

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5. Does Brassinolide Help Plant Use Photosynthesis?
Yes. Reasearch has indicated that brassinolide helps plant growth by helping it locate light [SUP](1)[/SUP] that is essential for plant life.
(1) Stitching Together a Receptor Reveals Plant Hormone Action [www.hhmi.org/news/chory2.html]

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[h=2]6. Does Brassinolide Help Plants Resists Cold, Drought And Salinity?[/h]Brassinolide strengthens a plant's immunity to stresses such as drought, salinity and cold [SUP](1)(2)[/SUP].Brassinolide can ensure a plant's steady growth and a longer growing season. Through all these factors brassinolide can ensure bigger and better quality plants.
1) www.chm.bris.ac.uk/motm/brassinolide/brassinolidej.htm
2) www.ibmc.up.pt/newsletter/IBMCNews_Dec03.pdf

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7. Does Brassinolide Help Plants Resist Disease?
Yes, brassinolide will improve a plant's ability to deal with diseases [SUP](1)[/SUP].
1) Turning on Disease Resistance [www.findarticles.com/cf_dls/m0DED/12_20/63802656/p1/article.jhtml]

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8. Can Brassinolide Help Plants Deal With Flooding?
One source [SUP]1[/SUP] reports that a foliar spray of 0.3 ppm of brassinolide helps plants deal with water flooding.

1. www.tnau.ac.in/tech/ricetips.pdf

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9. What Effect Does Brassinolide Have On Root Development?
A study [SUP]1[/SUP] concluded that Brassinolide increased the growth of the primary root by 90%.

1. http://abstracts.aspb.org/aspb1997/45/5180.shtml

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10. Does Brassinolide Affect Seed Germination?
One study [SUP]1[/SUP] concluded that Brassinolide improved the growth of the germinating seed. Another study [SUP]2[/SUP] has also concluded that Brassinolide has germination promoting effects.

1. www.leubner.ch/html/absbrassino01.html
2. www.pubmedcentral.nih.gov/articlerender.fcgi?artid=64877

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For more information on improved seed germination please take a look at Brassinolide, Chitosan, Fulvic Acid and Gibberellic Acid.
Order the Super-Grow Germination Pack!

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11. What Concentrations Of Brassinolide Are Used?
Concentrations of brassinolide are based on many different factors, such that hard rules are hard to define. In research brassinolide concentrations of 0.01 ppm [SUP](1)[/SUP] to 0.5 ppm have shown very good results [SUP](2)(3)[/SUP] across a wide range of plants.
1) www.actahort.org/books/239/239_54.htm
2) www.tnau.ac.in/cbe_crophy.html
3) www.tnau.ac.in/tech/ricetips.pdf

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12. When Should Brassinolide Be Applied?
It will vary from one plant to another, but one source [SUP]1[/SUP] recommends two different foliar sprays of brassinolide. The first at 25 to 30 DAS (days after seeding) and the second after 45 to 50 days. Alternatively, the same source suggests that for some plants the brassinolide should be sprayed at the beginning of flowering. Another source [SUP]2[/SUP] reports very good results when 0.1 PPM brassinolide is sprayed three times: once at the seedling stage, then at the flowering stage and finally at the fruiting stage.

1. www.tnau.ac.in/tech/ricetips.pdf
2. www.plant-hormones.com/tech.htm

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13. How Do I Store Brassinolide Powder?
Brassinolide powder should be stored in a cool dry place.

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14. How Do I Store Brassinolide After Mixing It?
Once it's in a liquid form brassinolide would normaly degrade very quickly. The best way to keep liquid brassinolide is to freeze it in a freezer tray. When you need to use the brassinolide just remove one ice cube from the freezer and let it melt.

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15. How Long Is Brassinolide Powder Good For?
It is estimated that the brassinolide powder can be kept for up to three years.

 

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Triacontanol is a naturally occurring plant hormone that acts as a growth promoter. Triacontanol raises plant yield by improving photosynthesis and cell division. The use of Triacontanol was the subject of several United States Patents including 4150970, 4230485 and 4333758.

User Feedback
[h=3]Roses - Triacontanol[/h]I bought Triacontanol 62% Pure: 2 grams.
I grow roses and have been using UltraBoost from a company called Primary Products. From what I have read, Ultraboost uses Triacontanol, so I figured I would try and make my own.
I bought 5 American Beauty roses all around the same size and health. I mixed several batches of the solution, 2 ppm, 10 ppm, 25 ppm and 100 ppm. I labeled each rose bush accordingly including one using the UltraBoost. The 25 ppm and 100 ppm bushes did the best, almost identical. So it appears in my small experiment 25 ppm is the way to go. No use using 100 ppm if the results are the same. Anyway, the surprise was that the 25ppm and 100ppm bushes over a weeks time actually doubled the size of new growth of the bush using UltaBoost. Go figure

[h=3]Preparing[/h][h=4]Dissolving[/h][h=5]Polysorbate 20[/h]Triacontanol powder is a wax-like flake that must be dissolved into a liquid. The best method of turning the Triacontanol powder into liquid is by using a food additive called Polysorbate 20. Among other uses Polysorbate is used in ice cream. You can find Polysorbate 20 on Ebay (www.ebay.com) and it's pretty cheap.
[h=5]Heating[/h]I placed one scoop (1/32 spoon) of the Triacontanol in a glass container with 50 ml of water and one scoop of Polysorbate 20. After heating it in the microwave and shaking it a bit the Triacontanol was completely dissolved.
[h=4]Diluting[/h]After the Triacontanol has been dissolved it's important that you add the solution to water and not the other way around. In this case we added enough to make 0.1 liters of a 100 PPM solution.
[h=3]Applying[/h][h=4]Foliar Spray[/h]Triacontanol is usually applied as a foliar spray and the biggest variable is what concentration to use, which is calculated in PPM (Parts-Per-Million). We have an on-line PPM calculator.
One published recommendation [SUP](1)[/SUP] has been to spray a 1 to 2 PPM triacontanol solution at two different times. The first is 15 days after transplanting the plant and the second time when the plant is at full bloom.
Another study [SUP](2)[/SUP] tested triacontanol concentrations of 10 PPM and 15 PPM. It concluded that triacontanol improved flower initiation, pod setting and retention of flowers. It also reduced fruit drop.
A third study [SUP](3)[/SUP] on a variety of rice concluded that a foliar spray of 2PM at 25 and 65 days after transplant greatly improved crop yield.
1. www.tnau.ac.in/tech/hortcg2004.pdf
2. www.cropresearch.org/pages/rocarchivevol3.no.1.htm

Frequently Asked Questions
1. What Is Triacontanol?
2. What Are The Effects of Triacontanol?
3. In What PPM Range Should Triacontanol Be Applied?
4. Is Triacontanol A Natural Substance?
5. How Does Triacontanol Work?

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1. What Is Triacontanol?
Triacontanol is a plant growth stimulator, also known as melissyl alcohol.
http://www.exoticnatural.com/triagro.htm

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2. What Are The Effects of Triacontanol?
Different studies will give different results due to different factors such as the type of plant its tested on. One study reported that triacontanol improves photosynthesis and plant yield by a much as 100% [SUP](1)[/SUP]. Another reports improvements of 15% to 30% [SUP](2)[/SUP]. A third reports that small amounts will improve vegetable crop yields by 30 to 60% [SUP](3)[/SUP].
1) www.carbonkick.fi/growingsystem/pressrelease_eng.doc
2) www.cftri.com/aboutus/about1.html
3) www.bmi.net/roseguy/boosters.html

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3. In What PPM Range Should Triacontanol Be Applied?
Please take a look at the instructions section.

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4. Is Triacontanol A Natural Substance?
Yes! It was first discovered in plants [SUP](1)[/SUP] in the 1930's. it's importance in plant growth and health was identified from this discovery.
1) www.carbonkick.fi/growingsystem/pressrelease_eng.doc

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5. How Does Triacontanol Work?
Triacontanol research does not have a long history, but does indicate that one of triacontanol's action is in improving photosynthesis. Since light is a primary source of nutrition the benefits of using triacontanol are obvious. It will also increase cell division [SUP](2)[/SUP].
1) www.carbonkick.fi/growingsystem/pressrelease_eng.doc