Just an update. No roots and no seedlings. Other chemicals have been ordered but I mostly need caps. I have to expand again and do not have the containers (the caps) I need to do so but I can't see paying a buck a cap, there has to be another way. The larger containers, though valuable are over 4 dollars each, considering that if I continue this, I will have need for hundreds if not thousands of jars, I have to come up with another solution.
I lost 8 jars to contamination from the original 70 and every one of the rooting experiments show solid little plants...with no roots, I am not worried. I soaked some seeds in water for 24 hours and placed them between napkins and plates and put them on my fridge. Still nothing. I have to get the control working before I will extend the experiment to the sterile tubes as it is a bother to fill them with STG and sterilize them for seeds that are evidently dead. I have 9 Hog seeds but I don't know if I want to sacrifice them for this.
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What you see above is three new cuttings from a few days ago, they are three different strains of unknown origin. I want to see if there will be problems establishing various strains as I discussed earlier. They look pretty good.
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There is a duplicate here but these are all our original lemon skunk cuttings, scroll back if you wish and look at how they have progressed, remember that these are all cuttings off of cuttings that have grown themselves. I could easily take three cuttings off of each of thse but of course, we are waiting for them to root instead. Had I been interested in continued multiplication of the Lemon Skunk I think I would have, 60 of them at this point, and shortly thereafter, 180. I can no longer claim to be doing all of this under 17 watts and in under 4 square feet. I added two more shelves. Now I can potentialy have 210 moms under 60 watts of light in 12 square feet (unless I place more than one plantlet in each jar - othewise we have the potential of having 630 "moms" or "clones". Notice what I've been talking about, they seem to be solidifying, growing more robust.
I think we can now safely say that an amateur, with a modicum of experience in some other form of aseptic conditions for the cost of a couple of electronic balasts, can establish and multiply almost any strain of cannabis.
Another pass at rooting should work, I am unconcerned.
I believe I have an automated solution for hardening off. (but I am concerned)
I have yet to:
Establish using a seedling
sex an unrooted plantlet
sex a rooted plantlet
Root
Harden
create an artificial seed
Tending to parameters of light and heat are on the agenda, I have said that I suspected that the plantlets were not getting enough light so I propped a few of them up a few inches on a box. They grew greener than the controls and they seemed to grow more quickly but this is subjective and counter to everything I know about these proceedures. It is still cool here and I am still hesitant to bump the temperature of the room up with a 1000 watt room heater. My lab runs a rather large HEPA filter continuously with the exhaust vented and deflected in order to minimise rapidly moving air. I blocked the central heating vent to the room so if the rest of the house is being heated, dust would not be thrown into the room. Because of that, the room's temperature is very different than the rest of the house, the only way I can manage it is with an electric heater. We have one of the highest electrical rates in the country here and I HATE paying the higher tiered bills. But my experiments show that optimum temperature is between 80 and 85 degrees.
Heating mats do not work as I had hoped because I cannot manage them. I have a thermostat connected to the mats but I don't know where to put the sensor, so far, placing it on top of a vessel simply keeps the mat on all the time.
La
Here is my soon to be modified super lab - 50 here, 12 up top and another 10 below. 70 moms or 70 clones depending on how you look at it. Mind you I am counting only one plantlet in each jar and that isn't the case with many of these. After the establishment phase one could easily have 3 plantlets in each jar. Now that is 150 clones on one shelf. 
These are only a few days old. Dank is using non-vented boxes and I like the idea of more realestate but not for starting single plantlets. I lose one I lose them all - except that I have had some sucess recently with rescuing a single one if I catch the contamination in time and if I happen to have a fresh, unused jar of media hanging around. I am hoping along with an orbital tilt plate (more on this soon), I can get a new fridge. If I do I intend to see if I can store media in the fridge to see if the chemicals can be made to last longer. I tried freezing the media in the same interest - don't bother, it doesn't work.
