Mitochondrial DNA (mtDNA) tissue propagation revisited

[Lavawolf]

When a great scientist tells you your idea is great, believe him; when a great scientist tells you your idea is impossible, never believe him.
Upon my first visit to rollitup I found a few brave guerrilla breeders wanting to know more about tissue culturing herb, along with the typically larger number of naysayers.
Is there anyone out there seriously wanting to learn how to access mitochondrial DNA (mtDNA), for developing strains no one else has ever seen; for taking our art to a new HI; for separating ourselves from the ever-growing larger masses of entry-level growers; perhaps, even, saving our families from the continuing economic decline that most of the rest of North America's citizens now endure...yaddayaddayadda...?
No one says mtDNA tissue culture will be easy...Ha! We'll have to learn new things, spend some money not at the local hydro-store, start taking serious lab notes we all can open-platform between each other. And lots more, too. But the end result can be strains no one else has. New strains that through recurrent-backcrossing can be optimized, and stabelized. Strains that most everyone will want but only those who produced them will control access-price.
Communicate this amoung yourselves, e-talk with me, time'z a'wasting.
Lavawolf

 

[canndo]

I've looked into it. I think that most of it is doable but I am unsure as to how one would determine what string is of value and how one would capture that snippet for future use.

 

[Wolverine97]

Way over my head, but interesting.

 

[canndo]

Woverine, there are several ways to implant portions of the dna of a plant. My understanding is that one can use a gun that shoots tiny particles or you can use a particular virus. There are companies that will load your particular strand into that virus and the virus will implant it into the DNA of your plant. Then you have to extinguish the virus and go on to duplcate the plant with the new dna sequence. At least that is how I understand it. Fine, now what sequence do we want in our plant?

 

[Wolverine97]

Woverine, there are several ways to implant portions of the dna of a plant. My understanding is that one can use a gun that shoots tiny particles or you can use a particular virus. There are companies that will load your particular strand into that virus and the virus will implant it into the DNA of your plant. Then you have to extinguish the virus and go on to duplcate the plant with the new dna sequence. At least that is how I understand it. Fine, now what sequence do we want in our plant?

Jesus. Science scares the hell out of me sometimes.

 

[hoagtech]

Isnt that what the tissue culture kits accomplish? A sealed environment with a hormone additive?

 

[Wolverine97]

Isnt that what the tissue culture kits accomplish? A sealed environment with a hormone additive?

No........
This would actually alter the DNA of the plant.

 

[DankBudzzz]

Isnt that what the tissue culture kits accomplish? A sealed environment with a hormone additive?

I am a lab technician and I've done work with violets, mums, lillies, potatoes, strawberries etc. using micropropagation and sterile agar cubes to grow out plants. Using a series of bleach and alcohol along with water baths you sterilize the tiny cut leaf portions and technically you can grow dozens of clones per tiny portion of a leaf...We have a diurnal chamber that is lined on all sides with T5s and you can control the phot period, temperature, humidity level and there is absolutely no need for water as the agar has additives (horomones) like auxins and gibberellins that normally are produced and used by plants to grow. However this allows tiny tissue fragements to grow into large plants.

It doesn't change the DNA by adding horomones.

The gene gun you speak of is a new technology to genetically modifify plants and aninmals to get desired traits...One way to do this is by using a clong vector such as a virus minipulated to express certain proteins which in turn will insert itself into the host plasmid or chromosomal DNA and alter the effects of the genes it disrupts. By targeting specific known genes gor grwoth regulation and horomone production we can produce superior plants with all desired effects...IT takes alot of time and research to do this but this is the future of growing...

 

[DankBudzzz]

Gene guns can use tiny ionic particles like Copper to penetrate tissue without causing extensive damage and to insert DNA into the host in the most effective and quickest way possible.

 

[Wolverine97]

Gene guns can use tiny ionic particles like Copper to penetrate tissue without causing extensive damage and to insert DNA into the host in the most effective and quickest way possible.

Would the new plant be a chimera or a hybrid?

 

[Wolverine97]

I am a lab technician and I've done work with violets, mums, lillies, potatoes, strawberries etc. using micropropagation and sterile agar cubes to grow out plants. Using a series of bleach and alcohol along with water baths you sterilize the tiny cut leaf portions and technically you can grow dozens of clones per tiny portion of a leaf...We have a diurnal chamber that is lined on all sides with T5s and you can control the phot period, temperature, humidity level and there is absolutely no need for water as the agar has additives (horomones) like auxins and gibberellins that normally are produced and used by plants to grow. However this allows tiny tissue fragements to grow into large plants.

It doesn't change the DNA by adding horomones.

The gene gun you speak of is a new technology to genetically modifify plants and aninmals to get desired traits...One way to do this is by using a clong vector such as a virus minipulated to express certain proteins which in turn will insert itself into the host plasmid or chromosomal DNA and alter the effects of the genes it disrupts. By targeting specific known genes gor grwoth regulation and horomone production we can produce superior plants with all desired effects...IT takes alot of time and research to do this but this is the future of growing...

Wow to the last paragraph.

 

[canndo]

Isnt that what the tissue culture kits accomplish? A sealed environment with a hormone additive?

I am certain I could alter the DNA of the plants in my collection using certain chemicals. I am also certain that I would have no control over what was changed.

 

[DankBudzzz]

I don't know the entire background to it, just that they are used to alter the DNA and in my opinion you could easily create strains and desired characteristics from numerous strains...Technically it would be a genetically modified mutant

 

[tardis]

I am a lab technician and I've done work with violets, mums, lillies, potatoes, strawberries etc. using micropropagation and sterile agar cubes to grow out plants. Using a series of bleach and alcohol along with water baths you sterilize the tiny cut leaf portions and technically you can grow dozens of clones per tiny portion of a leaf...We have a diurnal chamber that is lined on all sides with T5s and you can control the phot period, temperature, humidity level and there is absolutely no need for water as the agar has additives (horomones) like auxins and gibberellins that normally are produced and used by plants to grow. However this allows tiny tissue fragements to grow into large plants.

It doesn't change the DNA by adding horomones.

The gene gun you speak of is a new technology to genetically modifify plants and aninmals to get desired traits...One way to do this is by using a clong vector such as a virus minipulated to express certain proteins which in turn will insert itself into the host plasmid or chromosomal DNA and alter the effects of the genes it disrupts. By targeting specific known genes gor grwoth regulation and horomone production we can produce superior plants with all desired effects...IT takes alot of time and research to do this but this is the future of growing...

uh oh. Now don't get me wrong, I am pro science and knowledge, I think we should be using stem cells in our science to benefit the survival of mankind on our ever changing planetary environment, BUT once you start PROGRAMMING plants or animals with DNA instead of letting the DNA traits rise from reading the current environment, don't you create an imbalance in that plants long term ability for its offspring to survive on a planet who wasn't its creator? Isn't there some sort of natural survival mechanism that vanishes when its the conscious mind, and not the nature of nature that influences what dna traits are expressed? Isn't the danger say we build the perfect potato which is as incompatable with true nature as say Apple software on a windows computer, and that winds up becomign cloned and the only potato used by industry and thus becomes the dominant potato leading to a potato famine for humanity in 500 years?

Is it me or has the last 200 years of humanity been one big middle finger to the survival of future human generations? Its like the Eyptians said "They will be forced to remember us because of the massive structures we have put upon the earth." and modern human culture is like "screw that, they'll remember us cause we doomed and cursed them, they wont be able to forget us!"

 

[DankBudzzz]

It's evolution...I would say about 50% of all products are GM in some way troughout their life cycle or have additives that have been modifed. Examples. Most tomatoes grown have cold resistance genes which were injected after being isolated and purified from certain fish who live in depths where body temperature control is vital for survival..This allows crops to be produced even when frost hits or in the winter. Most wheats and barley and crops being grown for mass production are genetically modified to increase yield and ensure no crop damage due due to bacterial invasions etc. The amount of money lost due to diseases and other environmental factors every year is in the hundreds of billions...With these crops they can produce superior crops .

Sorry to ramble on but I have a have one last story...My professor in University years ago discussed alot of his thesis work with us and he along with his collegues over a few year period designed flowers which were genetically designed to bioremediate oil spills and chemical spills. The could actually suck up the oil and contaminants from the soil and process them to help them grow and help vegetative life thrive in that area along with cleaning up the harmful chemicals that have potential to desimate the water supply and plant life.

It's really a matter of preference but the work being done in these fields is amazing. For example, you can actually buy fish that have been tagged with GMP which is one of the most commercially used flourecent tag. However it is incorporated into there DNA so they actually flourese when the lights are off.

 

[canndo]

Dank, I believe the "flavor saver" tomato was a failure and the fish genetics are not included in current hybrid varieties. No amount of cross breeding will ever place a fish gene in a plant. It is these safeguards that protect our biosphere and we are short circuiting it. There is nothing in evolution that would do what you say and it is unwise to proceed without legitimate safegards for the ecosphere, the biosphere and mankind in particular. So far large corporations who's only legal obligation is to their stockholders are judging what is safe for our planet, this is an entirely unwise plan.

 

[tardis]

However I have no problem with the creation of Biodomes in order to test new species and balance the environment modifying dna in the variables as long as that pollen is contained and doesn't get out, treated like fort knox treats their gold. As long as they don't let Pauly Shore in i'm fine with it. It would benefit humanity greatly to create foodsources previously unable to be grown in nature.

however altering what IS natural is a dangerous game which if the wrong roll of the dice happens all humanity could be wiped out in a matter of months.

 

[DankBudzzz]

Imo, you guys just don't know any better. I'm not claiming to be a expert or even close to one on the topic but it's a fact that genetically modified foods have been passed by the FDA on numerous occasions. As for the tomato, they have been FDA approved since 1994 so It was not a failure. Even my local greenhouse that produces hydroponic vegetables in the winter uses these strains as they are commonly available in seed form.

Bananas in an unmodified form are full of large black hard seeds. The ones we eat have been modified so the expression of these traits are minimal and allow the overexpression of fruit tissue over seed....


If you believe that genetic engineering is so wrong then consider the medical advantages that genetic therapy can have. Diseases that devasted millions in the past are now curable due to research into genetically modified cloning vectors. Genetic diseases can such as Turners can now be cured or due to vectors that can simply knockout that specific gene without disturbing the rest of the genes...Again it's all a matter of preference; but in controlled environments.

I judged a national science fair and a kid in grade 6 had developed a way to not only cure cancer but take the rapidly dividing cells and convert them back into stem cells so they could be used to repair the damaged tissue. Chemotherapy and other treatments act by killing all rapidly dividing cells so in the process all immature stem cells and undifferentiated dividing cells are killed which is why it is very hard on your body...He was in grade 6 and managed a $500 000 government grant with 24 researchers working under him. He had his own laboratory completely built and funded by the government. He won overall for all categories in the fair and walked away with $80 000 in cash and $40 000 in scholarships..

 

[tardis]

Yes this modifications led to things like High Fructose Corn Syrup from highly modified corn which has lead to many severe life shortening diseases in our modern world today. The full effects and harm of high fructose corn syrup in our diet has not fully been realized yet but they know it is not good.

I agree, i'm all for the use of science to benefit mankinds survival, but I am not for the use of science to benefit profits at the risk of killing people and or all life as we know it.

 

[DankBudzzz]

As in any other aspect of life and business, there are people who are willing to break the rules to profit, Of course there is the possibility of developing something detremental to society but that is why they have agencies such as the FDA or CFIA/AFIA. There are very very strict regulations and there must be abundant statistical data and testing before they would approve anything for public use.

I think it's just what people don't know scares them. Theres so much that hasn't been discovered yet but somebody has got to do it.