Plant Cloning Issues, What's Going On?

RockStonedJesus

Active Member
I seem to be having a rough patch with my clones, perhaps I'm over-thinking it, or perhaps there's something seriously wrong?

I've got three plants which I took cuttings off - of two are new genetics / popped from seed, while the other one is 2nd generation cuttings. The second generation cutting is one of 10 I took, with a 10/10 cutting success rate. The others went to friends.

About a month ago, I took cuttings from all three of my plants - totaling to about 30 cuttings. My proceedure was standard:

  • Soak Eazy Plugs in tap water with a low dose of H2O2 (pH around 6.5)
  • Allow the plugs to drain any excess water
  • Using a skewer, gently open the holes in each of the plugs
  • Take cuttings from plant, fixing up cutting with a slight angle on the cutting and very light scraping of the outer skin of the cutting
  • Trim off 1/3rd of the leaf tips
  • Dip the cutting in GT's CloneX Purple Gel
  • Place the cutting into the Eazy Plug, ensuring it's snug in the plug
  • Put the humidity dome ontop once all cuttings are complete

This first round, I think I stuffed up with too much moisture, as I was foliar spraying them once a day + didn't leave the humidity dome open to allow a more regulated humidity inside the dome. All these cuttings ended up rotting away, zero roots formed. There was some white fuzzy mold on a few of the stems. They were doing well for a week and a bit, but still had no roots, they all ended up dying on about week 2. I did open the domes for a few minutes every day, but clearly, that wasn't enough.

I then did a second round of clones, another 30 clones, after the OG's ended up dying/rotting. This time, I changed things up a little, and ensured the humidity domes were partially opened, allowing for more exchange of air, and better humidity control.

After about a week, there was still no signs of rooting - further, I noticed some of the stems were kind of rotting/shrinking, but the foliage was still 100% healthy. Coming to week two, they all started slowly dying off, further rotting away. They were a complete write off. There was no way the stems were going to push roots at that point.

Here we are today - I managed to get a Turbo Kloner from Facebook Marketplace for cheap! Woo. I sanitized it with H2O2, rinsed it, and prepared it for growing. It was already very clean, and was left unused for some time, so I don't think there are any issues there, but, after about a week, all 24 clones looked like they weren't going to take off.

There didn't appear to be any callousing on any of the stems, and yet again, it looked like the stems were shrinking, rather than bursting root nubs. WHAT?

A little after a week, coming to 2 weeks, there were still no roots forming, rather, it looks like the stems were rotting/dying back, in a completely different environment.

What the heck is going on? The only thing I can think of is either:

  • Temperatures too high (24-29 degrees C - temperatures are heating up here)
  • Water quality from the tap is causing dramas
  • My H2O2 is causing issues (even at dilute rates, could it be causing issues? I believe there is a stabilizer chemical added to my H2O2, perhaps this is causing me dramas...? Clutching at straws?)
  • My mother plants (all three), are somehow infected with something?

I have since moved my Turbo Kloner into a temperature controlled room, aiming for about 23/24 degrees C, to help keep the water cool. I also currently have it on a 2 minute on/15 minutes off timer for the water pump, so they're not constantly wet. I also threw out some of the older cuttings, and put some fresh cuttings in.

It has now been a week later since the new cuttings were put in the Turbo Kloner, and still, I'm not seeing any success. Infact, it looks like the stems may be rotting also? They're still on the green side, but they look like they're going to shrink like the others, and die off.

I'm at a complete loss at the moment, it's getting quite stressful. I might be over-thinking it, but I've watched and read hundreds of videos/forums/threads on cloning just to ensure I'm not stuffing anything up, and somehow, things just don't seem to be getting any better. My mother plants are super healthy. And to add insult to injury, I flipped the plants into flowering, not expecting to have a 100% failure rate like this.

I've attached a few pics from the Turbo Kloner.... I've tried all sorts of cuttings, and techniques (IE: Terminate the cutting just below the node, keeping some stem below the node, lightly scrape off, no scraping, flat cut, angle cut, etc, etc), absolutely 0 luck. I worry these cuttings will also rot and die off. The stems above the clone collar are still alive, but as soon as you look at the stem below the collar, it's shriveled, small, looks necrotic.

Anyone can point me in the right direction will be sincerely appreciated.

I do have distilled water which I will switch to if I end up with another complete loss. Do I need to add nutrients to the water? Hormones? Something to help speed up the rooting process other than my CloneX IBA?
 

Attachments

go go kid

Well-Known Member
you just take some cuttings and put them in a glass of water, place on a window cill or under a 50watt led/fluro light or in your tent , change water every other day and they will take, no need for all this plugs n such
 

RockStonedJesus

Active Member
Use bleach (sodium hypochlorite) instead of h2o2. You need to add peroxides two times a day for it to be active for 24h. It has very short residual effect of 12 hours compared to days with bleach.

You find the chlorine dilution calculator in my signature: http://foodsafe.ca/dilution-calculator.html. You can make a 3ppm solution and add back every 3rd day.

Good luck!
Thanks mate. Would this be fine to run in my Turbo Kloner, with the clones in it? Or is this more so to sterilize the system for the next run?

Furthermore, any idea regarding the stems shrinking - is this normal, or are they on their way out?

you just take some cuttings and put them in a glass of water, place on a window cill or under a 50watt led/fluro light or in your tent , change water every other day and they will take, no need for all this plugs n such
I did have a couple of cuttings in a glass of water, the stems turned white, assuming this was them ready to push roots out? I did neglect them and didn't change the water as often as I should have. Though it took a good 2 weeks, and still no roots.. So no idea.

Maybe I'm clutching at straws, but I did read that it's likely my plants have a systematic Fusarium infection - this could explain the first round of cuttings, where I visually noticed some white fuzz and tiny orange-looking scales on the stem of the cutting...
 

Wastei

Well-Known Member
Thanks mate. Would this be fine to run in my Turbo Kloner, with the clones in it? Or is this more so to sterilize the system for the next run?

Furthermore, any idea regarding the stems shrinking - is this normal, or are they on their way out?



I did have a couple of cuttings in a glass of water, the stems turned white, assuming this was them ready to push roots out? I did neglect them and didn't change the water as often as I should have. Though it took a good 2 weeks, and still no roots.. So no idea.

Maybe I'm clutching at straws, but I did read that it's likely my plants have a systematic Fusarium infection - this could explain the first round of cuttings, where I visually noticed some white fuzz and tiny orange-looking scales on the stem of the cutting...
It's for running in the cloner to combat Pythium that prevent roots from forming. Bleach is a weaker oxidizer than h2o2 and last longer, its therefore safer to use in practice. It's very unlikely that all cuttings are affected by fusarium wilt, it doesn't spread well in water.

Yes it works well and what I do. I only do water cloning were the cuttings sit in water(DWC cloner) with the same kind of neoprene rings you use in your turbokloner.
 

Woedae

Member
It's for running in the cloner to combat Pythium that prevent roots from forming. Bleach is a weaker oxidizer than h2o2 and last longer, its therefore safer to use in practice. It's very unlikely that all cuttings are affected by fusarium wilt, it doesn't spread well in water.

Yes it works well and what I do. I only do water cloning were the cuttings sit in water(DWC cloner) with the same kind of neoprene rings you use in your turbokloner.
That is really helpful to know. I've had mediocre results taking clones and straight into plugs. I've been looking at clone machines. Is there any advantage to an low pressure aero compared to the dwc style. All I keep seeing is heat issues with the aero, and they cost so damn much.
 

Wastei

Well-Known Member
That is really helpful to know. I've had mediocre results taking clones and straight into plugs. I've been looking at clone machines. Is there any advantage to an low pressure aero compared to the dwc style. All I keep seeing is heat issues with the aero, and they cost so damn much.
Not really IMO. Maybe slightly faster with a high pressure Aero cloner but with low pressure they perform the same. It's easier to keep temps down in a simple DWC cloner without any pump to heat up the water.
 

Woedae

Member
Not really IMO. Maybe slightly faster with a high pressure Aero cloner but with low pressure they perform the same. It's easier to keep temps down in a simple DWC cloner without any pump to heat up the water.
What water temp should be maintained? I'll definitely be using that bleach dilute calculator and plain tap water. My municipal water is loaded with chloride and chloromines, cal, mag, iron. Tests around 200ppm, but i don't know individual values. Should I aim for 2% bleach in the calculator, or do I even need to use bleach with tap?
 

coreywebster

Well-Known Member
Thanks mate. Would this be fine to run in my Turbo Kloner, with the clones in it? Or is this more so to sterilize the system for the next run?

Furthermore, any idea regarding the stems shrinking - is this normal, or are they on their way out?



I did have a couple of cuttings in a glass of water, the stems turned white, assuming this was them ready to push roots out? I did neglect them and didn't change the water as often as I should have. Though it took a good 2 weeks, and still no roots.. So no idea.

Maybe I'm clutching at straws, but I did read that it's likely my plants have a systematic Fusarium infection - this could explain the first round of cuttings, where I visually noticed some white fuzz and tiny orange-looking scales on the stem of the cutting...
Your first problem was neglect.

If you have a dome you can't be leaving it shut .
Lack of air exchange and movement with cause all sorts of issues.

After a few days you can usually take the dome off.


As for your rooting times, 7 days isn't long.

I've a cherry mac muffin throws roots in 5 days, a wedding cake takes 2 weeks and a gelato that takes 3-4 weeks.

That's all in the same aero cloner.
 

coreywebster

Well-Known Member
That is really helpful to know. I've had mediocre results taking clones and straight into plugs. I've been looking at clone machines. Is there any advantage to an low pressure aero compared to the dwc style. All I keep seeing is heat issues with the aero, and they cost so damn much.
You can buy the manifold with sprayers for cheap and make one yourself.

Most of the cost of ezcloners seems to be the res and tray.
 

Wastei

Well-Known Member
What water temp should be maintained? I'll definitely be using that bleach dilute calculator and plain tap water. My municipal water is loaded with chloride and chloromines, cal, mag, iron. Tests around 200ppm, but i don't know individual values. Should I aim for 2% bleach in the calculator, or do I even need to use bleach with tap?
Aim for 3ppm. You can go as high as 5ppm in water cultures.
 

Wastei

Well-Known Member
What water temp should be maintained? I'll definitely be using that bleach dilute calculator and plain tap water. My municipal water is loaded with chloride and chloromines, cal, mag, iron. Tests around 200ppm, but i don't know individual values. Should I aim for 2% bleach in the calculator, or do I even need to use bleach with tap?
Not that important. I put it at ambient temps and maybe 20w LED's, around 22C/71.6F water temp. You just need enough light to root, less is more.
 

RockStonedJesus

Active Member
Use bleach (sodium hypochlorite) instead of h2o2. You need to add peroxides two times a day for it to be active for 24h. It has very short residual effect of 12 hours compared to days with bleach.

You find the chlorine dilution calculator in my signature: http://foodsafe.ca/dilution-calculator.html. You can make a 3ppm solution and add back every 3rd day.

Good luck!
I've got Calcium Hypochlorite powder on hand, thoughts on this, or should I stick to Sodium Hypochlorite?

How long does it usually take before you visually see roots? I might setup a similar system, perhaps pop an air stone in there. See which I can get kicking-off the fastest.. Haha!

Your first problem was neglect.

If you have a dome you can't be leaving it shut .
Lack of air exchange and movement with cause all sorts of issues.

After a few days you can usually take the dome off.


As for your rooting times, 7 days isn't long.

I've a cherry mac muffin throws roots in 5 days, a wedding cake takes 2 weeks and a gelato that takes 3-4 weeks.

That's all in the same aero cloner.
That's 7 days with no roots, coming to 2 weeks with no roots. No signs, only the stems shriveling up. I don't think that's normal, is it?

Not that important. I put it at ambient temps and maybe 20w LED's, around 22C/71.6F water temp. You just need enough light to root, less is more.
I've got my room temperature dialled in to about 23 degrees C, with a portable AC unit. Though I still worry about those stems shrivelling up, they look like they're just going to rot away.. Will they still push roots? Or am I at a total loss again?
 

coreywebster

Well-Known Member
I've got Calcium Hypochlorite powder on hand, thoughts on this, or should I stick to Sodium Hypochlorite?

How long does it usually take before you visually see roots? I might setup a similar system, perhaps pop an air stone in there. See which I can get kicking-off the fastest.. Haha!


That's 7 days with no roots, coming to 2 weeks with no roots. No signs, only the stems shriveling up. I don't think that's normal, is it?


I've got my room temperature dialled in to about 23 degrees C, with a portable AC unit. Though I still worry about those stems shrivelling up, they look like they're just going to rot away.. Will they still push roots? Or am I at a total loss again?
The stems I can't judge from the pics.
The time frame, perfectly normal.
Some methods are faster than others
Some strains are faster/slower and Some are a bitch
 

RockStonedJesus

Active Member
The stems I can't judge from the pics.
The time frame, perfectly normal.
Some methods are faster than others
Some strains are faster/slower and Some are a bitch
Oh, for sure, I'm well aware! ;)

I just don't know what has gone wrong. I haven't had any issues cloning up until now.

Sometimes I do get a 100% loss rate, and other times I get a 100% success rate.

I've previously cloned this exact lineage of genetics. No issues, albeit a different time of the year (temperature-wise).

Is there a way I can know if the stem is dying or if it's okay? Because it has shrunk to 1/2 maybe even more of its original size. (The part of the stem being sprayed by the Turbo Kloner).

I just double-checked and I have my pump timer on 2 mins on, 15 mins off... Even though Turbo Kloner recommends 24/7 operation. I'm skeptical on the run-time, as obviously I don't want it to create an environment that results in longer rooting times. I'm about to pull my hair out.
 

coreywebster

Well-Known Member
Oh, for sure, I'm well aware! ;)

I just don't know what has gone wrong. I haven't had any issues cloning up until now.

.

I've previously cloned this exact lineage of genetics. No issues, albeit a different time of the year (temperature-wise).
.
think that's the nail being hit on the head.

I had 100% success rate for many years, then I moved house.

Slight change in the environment and I was struggling. (That's pre ezcloner)

Only thing that was different was the environment. Everything else was the same.

I can't say anything about the stems because it's not something I've ever noticed.

When I've suffered rot it's not shrunk the stem, it's just gone brown and mushy.
I've not had rot in my ezcloner but my temps are cold , its in my basement.
I run pump 24/7 , but I don't need to worry about overheating.
 

GenericEnigma

Well-Known Member
I use an aerocloner. It is all that has worked reliably in my conditions: very arid. I keep the water coldish, probably no higher than 18dC. No dome, no gel, no powder. Pump runs continuously. After 10-14 days (strain-dependent), this:

20240326_163701.jpg

If you are in an arid environment, I suggest putting your cloner in a cold corner and keeping the dome off.

Always very minimal lighting for rooting clones.
 

RockStonedJesus

Active Member
I use an aerocloner. It is all that has worked reliably in my conditions: very arid. I keep the water coldish, probably no higher than 18dC. No dome, no gel, no powder. Pump runs continuously. After 10-14 days (strain-dependent), this:

View attachment 5450786

If you are in an arid environment, I suggest putting your cloner in a cold corner and keeping the dome off.

Always very minimal lighting for rooting clones.
I'm coming close to the 14 days now for the second lot of clones, and really, am getting no where.

I've got the Turbo Kloner in a tent, in the corner, out of direct light, temperature controlled the room to about 23 degrees C.

Last night I sprayed out the Turbo Kloner water tank with some bleach, it fizzed up a little, perhaps some organic matter in the tank? Dunno. But I filled it back up with some distilled water (5L + about 1L of tap water as that's all I had), and popped about 3ppm of Calcium Hypochlorite.

Really not sure what's going wrong, because I've always had great success with cuttings, and to be frank, this really has me stumped.

The only thing I can think of, realistically, is if the plants have some sort of issue preventing the cuttings from rooting...?

As mentioned earlier, this particular strain has been cloned before (this is the second generation of clones), and I had absolutely no dramas getting them to root. Only now I'm struggling. All conditions are the same, nothing different, except the first batch of clones where the humidity was far too high in the dome box..

But I've been running no dome with the Turbo Kloner, and still not seeing much action happening root-wise. I worry, because the stems look like they're shrinking and dying off, they don't look like they're alive, they literally look like they're slowly dying off. The stems in the aerocloner are 1/2 the size of the stem above the collar, they don't look good. Is this normal?

Looking at the stem in your pic, it seems healthy, no issues. Whereas mine... Look like they're going to eventually rot...
 
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