Tissue Culture

[517BlckBerry]

I thought a breeder was doing it back in the day
I cant recall who

It might have been a dispensaries mother room or something.

If i have to search i will but i know it has been used for weed somewhere

If i a little hobby grower can want to try it
I only imagine someone has done it

Lol there is a youtube video where some little city kid like in his 20s that shows you how.to make the.mix at home

I'm still debating on what mix to try. I will most likely just order the TC Kit for cannabis, that a couple people posted, but I really want to try the specific recipe that I posted. Apparently a researcher found a way to get 95% success rates with her specific formula. Should be a fun experiment nonetheless. Come back and post some videos if you find em, I only found a couple that weren't really that helpful. The one guy though, Microclone, is on his shit. Looks like he knows whatsup

 

[Dr Magill]

I've done a lot of it - but not with cannabis. The nutrient mix is made to supply nutrients and rooting/shoot forming hormones. The base is agar - which is like gelatin. The medium is placed in sterile jars or tubes and autoclaved. Tissues from the plants are collected and sterilized - bleach works for this but I do not recall the %.
Nodes work as well as growth tips and leaf petioles. Some species of plants perform best with a tip cutting and some with a petiole. You will need to experiment.
The sterile treated tissue is placed into the growth medium under sterile conditions - like in a laminar flow hood. Not entirely necessary (pricey) but any contamination - any - will kill the cultures.
The tubes are sealed with breathable parafilm - you might recognize this from science class in hs - (culture has to breath) and are placed under lighting in a warm room with not a lot of outside air flow.
Over time the tissue forms a wad of callous which may or may not differentiate into roots and shoots. Success depends upon conditions in the tube and correct media formulation.
Cultures can then be separated under sterile conditions into additional tubes so now you have multiple micro-clones. Large numbers in a very small space. If successful you will have dozens, hundreds, and potentially thousands of small clones. The cultures can be open and further rooted by conventional means and maintained in your culture room.
I actually think it has potential to store hundreds of varieties in a gene bank type set up.
African violets are cultured very easily by this method - the petiole works well. I mention this because it might be good practice. You would be amazed at how many production nurseries use this method for a large number of plants they propagate. Been around since the 80's at least.
Good luck

 

[Cold$moke]

Recipes are out there it isnt new by any means
Just people dont want the hassle

But i think it has merits

 

[517BlckBerry]

I've done a lot of it - but not with cannabis. The nutrient mix is made to supply nutrients and rooting/shoot forming hormones. The base is agar - which is like gelatin. The medium is placed in sterile jars or tubes and autoclaved. Tissues from the plants are collected and sterilized - bleach works for this but I do not recall the %.
Nodes work as well as growth tips and leaf petioles. Some species of plants perform best with a tip cutting and some with a petiole. You will need to experiment.
The sterile treated tissue is placed into the growth medium under sterile conditions - like in a laminar flow hood. Not entirely necessary (pricey) but any contamination - any - will kill the cultures.
The tubes are sealed with breathable parafilm - you might recognize this from science class in hs - (culture has to breath) and are placed under lighting in a warm room with not a lot of outside air flow.
Over time the tissue forms a wad of callous which may or may not differentiate into roots and shoots. Success depends upon conditions in the tube and correct media formulation.
Cultures can then be separated under sterile conditions into additional tubes so now you have multiple micro-clones. Large numbers in a very small space. If successful you will have dozens, hundreds, and potentially thousands of small clones. The cultures can be open and further rooted by conventional means and maintained in your culture room.
I actually think it has potential to store hundreds of varieties in a gene bank type set up.
African violets are cultured very easily by this method - the petiole works well. I mention this because it might be good practice. You would be amazed at how many production nurseries use this method for a large number of plants they propagate. Been around since the 80's at least.
Good luck

Hey thanks man! That's some great info. Just made me even more excited to try this

 

[Sour Wreck]

interesting video, especially the talk about eliminating powdery mildew

 

[Sour Wreck]

i guess the plant hormones are responsible for multiple shoots. sounds like a custom recipe...

 

[Sour Wreck]

hmm, just can't find much so far on multiple shoots.

what i did find was a guy who puts tiny node pieces into tissue culture. i also saw another guy who put multiple pieces in one tube. said each plant required space of a quarter and 10ml of medium. this could be one method of storing, growing multiple future clone in one container.

 

[vostok]

LINK> https://forum.grasscity.com/threads/tissue-culture-micropropagation.998333/

http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf for a heavy pdf.

good luck

 

[Dr Magill]

Within that link is a description of the media used during the stages of propagation. If you will note take a look at how they treat the seeds prior to germination. That short section alone should give you a pretty good idea of the need for really good technique if you want success

 

[Sour Wreck]

here's a cool DIY idea for creating a sterile working environment

 

[517BlckBerry]

here's a cool DIY idea for creating a sterile working environment

I like that!!! This is most likely what I will make. Thank you guys so much for all the posts, great information! I'm so excited to try this I like the scientific stuff lol

 

[517BlckBerry]

interesting video, especially the talk about eliminating powdery mildew

thats crazy how he said it could rejuvenate the traits of a mother plant that normally degrade over time. very interesting this guy has great info

 

[vostok]

here's a cool DIY idea for creating a sterile working environment

is that idea not from the Simpsons opening sequence...?

 

[Sour Wreck]

here's another good one that is a bit all over the plants, but notice the very small pieces this guy adds to his tubes

 

[517BlckBerry]

here's another good one that is a bit all over the plants, but notice the very small pieces this guy adds to his tubes

Just started watching that one hahah. Seems like some good info as well. I wish I could find more from that guy with the orange hair he clearly has some amazing knowledge on plants

 

[Sour Wreck]

this series really shows the whole process

episode 1

 

[fearnoevil]

Haven't been able to find any videos but I have been finding some people who research&study cannabis using these methods and having great results. Making tons of plants off one small cutting essentially. That's my ultimate goal with this is to make as many plants as I can from the smallest cuts possible. Even if it takes months, it would be worth it. I imagine it would work like most other plants are working but only time will tell... I will post as soon as I start it

I remember looking into this several years ago and what I came away with was this: IF it was really an affordable and not prohibitively difficult/expensive way to go, then a lot of people would be doing this, as in the big commercial growers. There are a lot of big brains involved on the commerical side of this business, and it is a business especially now with legalization cropping up everywhere.

Now maybe my info is dated, but so far I haven't heard a peep that it's happening, maybe though in some labs that are doing genetic research (could possibly have a use in genetic manipulation with recombinant DNA and molecular cloning, idk ;?). But it also sounds interesting, if nothing else then for the pure gear-headedness of it. Let us know what you find out dude.

 

[fearnoevil]

interesting video, especially the talk about eliminating powdery mildew

HAHAHAAAA, "all their creepy genetic modification stuff", that made me laugh, and I couldn't take him seriously after that. Sounds like a typical organo-nazi Luddite - welcome to the 21st Century, genetic modification is all around us, and yes we know the old saw, but it's true, when you do selective breeding, you ARE doing genetic modification that would otherwise not occur in NATURE, which is what these types obsess with, lol.

A lot of what Monstanto and others do is not creepy - if you splice a fish gene with a corn gene (both of which we've eaten separately so no big dif combining the two) to get a frost resistant plant, the bottom line is you are improving global food production and feeding more people by protecting the food supply from one of natures destructive forces, same goes for drought resistance, disease resistance etc.

Although if you're not living in the Third World and wondering where your next meal is coming from, then you may not appreciate the value of this (but with 10 BILLION people globally in about 40-60 years, many of you will better understand this some day). What does sound scary (and I mean seriously) is what Craig Venter's up to, with the whole synthetic life research, creating shit that's never existed before - like nukes before this, it could be a boon as well as a possible doomsday creation,

 

[517BlckBerry]

HAHAHAAAA, "all their creepy genetic modification stuff", that made me laugh, and I couldn't take him seriously after that. Sounds like a typical organo-nazi Luddite - welcome to the 21st Century, genetic modification is all around us, and yes we know the old saw, but it's true, when you do selective breeding, you ARE doing genetic modification that would otherwise not occur in NATURE, which is what these types obsess with, lol.

A lot of what Monstanto and others do is not creepy - if you splice a fish gene with a corn gene (both of which we've eaten separately so no big dif combining the two) to get a frost resistant plant, the bottom line is you are improving global food production and feeding more people by protecting the food supply from one of natures destructive forces, same goes for drought resistance, disease resistance etc.

Although if you're not living in the Third World and wondering where your next meal is coming from, then you may not appreciate the value of this (but with 10 BILLION people globally in about 40-60 years, many of you will better understand this some day). What does sound scary (and I mean seriously) is what Craig Venter's up to, with the whole synthetic life research, creating shit that's never existed before - like nukes before this, it could be a boon as well as a possible doomsday creation,

I disagree with the whole GMO thing in food... There's science behind it that I don't fully understand, but I tend to go with my gut instinct on GMOs. GMOs are not natural, they do not hold the same nutritional benefits as natural genetics in my opinion, and GMO is a way for corporations to control the food industry and that is unacceptable. There has been a lot of tests done where mice/ have been fed GMO corn and other things for periods of time leading to development of tumors. This is not the same practice as selective breeding... not by a long shot... This is a conversation for another thread though.

 

[517BlckBerry]

I remember looking into this several years ago and what I came away with was this: IF it was really an affordable and not prohibitively difficult/expensive way to go, then a lot of people would be doing this, as in the big commercial growers. There are a lot of big brains involved on the commerical side of this business, and it is a business especially now with legalization cropping up everywhere.

Now maybe my info is dated, but so far I haven't heard a peep that it's happening, maybe though in some labs that are doing genetic research (could possibly have a use in genetic manipulation with recombinant DNA and molecular cloning, idk ;?). But it also sounds interesting, if nothing else then for the pure gear-headedness of it. Let us know what you find out dude.

I thought this as well. Why aren't more people doing it, it must be something that isnt very practical... only way to find out is give it a whirl i guess lol. I run my operation in strange ways and I will do something I enjoy, that I feel is Natural, over something more beneficial simply because I enjoy it. This might be one of those things that isn't more progressive but certain enjoyable lol. Love the science stuff

If it DOES end up being practical, I will certainly post all of my findings/recipes and get it out there. I'm still shocked that I never even heard of this process before recently.. Would've tried this a long time ago lol