Gender is set in the new plant at the time of fertilisation by its inheritance of either the X or the Y chromosome from the male (staminate) plant. With germination of the seed, the environment comes into play. Heritage sets the genetic program, but the environment can influence how the program runs. (Sexual expression in Cannabis is delicately balanced between the two.) The photoperiod, for example, controls the plant's sequence of development. Also, the plant's metabolism and life processes are dependent on growing conditions. When the environment does not allow a balance to be maintained, the normal genetic program may not be followed. This is mirrored by abnormal growth or sexual expression.
Many environmental factors can cause intersexes and sexual reversals. These include photoperiod, low light intensity, applications of ultraviolet light, low temperatures, mutilation or severe pruning, nutrient imbalances or deficiencies, senescence (old age), and applications of various chemicals (see bibliography on sex determination).
The photoperiod (or time of planting using natural light) is the most important factor to consider for normal flowering. In 1931, J. Schaffner (105) showed that the percentage of hemp plants that had confused sexual characteristics depended on the time of year they were planted. Normal flowering (less than five percent of the plants are intersexes) occurred when the seeds were sown in May, June, or July, the months when the photoperiod is longest and light intensity is strongest. When planted sooner or later in the year, the percentage of intersexuals increased steadily, until about 90 percent of the plants were intersexual when planted during November or early December.
Marijuana plants need more time to develop than hemp plants at latitudes in the United States. Considering potency, size, and normal flowering, the best time to sow for the summer crop is during the month of April. Farmers in the south could start the plants as late as June and still expect fully developed plants.
If artificial light is used, the length of the photoperiod can influence sexual expression. Normal flowering, with about equal numbers of male and female plants, seems to occur when the photoperiod is from 15 to 17 hours of light for a period of three to five months. The photoperiod is then shortened to 12 hours to induce flowering. With longer photoperiods, from 18 to 24 hours a day, the ratio of males to females changes, depending on whether flowering is induced earlier or later in the plant's life. When the plants are grown with long photoperiods for six months or more, usually there are at least 10 percent more male then female plants. When flowering is induced within three months of age, more females develop. Actually, the "extra" males or females are reversed plants, but the reversals occur before the plants flower in their natural genders.