• Here is a link to the full explanation: https://rollitup.org/t/welcome-back-did-you-try-turning-it-off-and-on-again.1104810/

Gas Chromatograph

C

CannaReview

Well-Known Member
Ok so that means I'll have to get say 3 samples of three different grows/strains. Send each pack of 3 to a GC-FID lab and another pack to a HPLC lab and see what the results are. Unless I'm too stupid to learn some of the methods I can still see this as a cheap consumer oriented cannabis tests.
 
CalyxCrusher

CalyxCrusher

Well-Known Member
My only question would be where to find a lab with all the HPLC equipment which has been properly calibrated specifically for testing cannabinoids. That's the tricky part
 
C

CannaReview

Well-Known Member
CalyxCrusher said:
My only question would be where to find a lab with all the HPLC equipment which has been properly calibrated specifically for testing cannabinoids. That's the tricky part
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I'll contact these guys although would have to be secret squirrel when sending it over the border lol. I did find an article about a HPLC lab in Toronto yesterday but can't find the link atm.
 
Flash63

Flash63

Well-Known Member
I call her the hightest because she is among the highest THCA plants I've seen on a validated HPLC method, testing at over 32% THCA. Yes we ran samples multiple times to confirm the result using check standards to ensure calibration of the equipment- keep in mind this is THCA not THC, so after decarboxylation the mother is in the ~29% THC range. Chimera posted this a couple of weeks ago....not sure if you live in BC?
 
C

CannaReview

Well-Known Member
Flash63 said:
I call her the hightest because she is among the highest THCA plants I've seen on a validated HPLC method, testing at over 32% THCA. Yes we ran samples multiple times to confirm the result using check standards to ensure calibration of the equipment- keep in mind this is THCA not THC, so after decarboxylation the mother is in the ~29% THC range. Chimera posted this a couple of weeks ago....not sure if you live in BC?
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yes BC
 
The Hippy

The Hippy

Well-Known Member
CannaReview said:
Yes own.
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Well if I was near you I would shop at your store. I hope get more than your share of business.
That's another thing the mmar is good for small business and grow shops. Look at all the millions spent by patients at independent businesses like your. Much better money spent keeping you, supplier and manufacturers than spent at Lp.
I'm concerned for grows shop because if we loose this deal how many shops will close ? Shops will be easy targets for cops as basically if no med growing is allowed, then really no one should be in grow shops. I know, some folks grow vegetables and other plants. But lets be honest, home growing of MJ is the biggest chunk f the business.
The MMAR was good for small business and the mmpr is good for select golden blow job big business. The little guys always get the short end of the stick.
I shop at independent Canadian owned business whenever I can ......but it's getting harder all the time.
I respect you Canna and wish you luck and good support in the shop. Maybe if your comfortable you could mention your shop and where it is. Maybe some folks on here nearer to your can send some business your way. If you don't wanna say I totally understand as that may be too personal.
 
C

CannaReview

Well-Known Member
The Hippy said:
Well if I was near you I would shop at your store. I hope get more than your share of business.
That's another thing the mmar is good for small business and grow shops. Look at all the millions spent by patients at independent businesses like your. Much better money spent keeping you, supplier and manufacturers than spent at Lp.
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I'm quite jaded (fuck haven't use that word since I heard it used in Jaded Raver lol) with this industry. Its a stagnant, lets bring in the cheapest shit we can industry on top of all the snake oil products. Also because of the nature of the business there's some crazy cut throat pricing going on in areas where there's a concentration of stores.

I'm concerned for grows shop because if we loose this deal how many shops will close ?
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Shops would probably be better with full illegal or full legal. Stores were not doing that good with the amount of licenses that were around especially in BC. Yes at the begging when the doctors started to sign left and right there was a mad rush of people buying equipment but that only lasted for a year of so till the price of the crop fell from $2500 to $900/lb over night.

Shops will be easy targets for cops as basically if no med growing is allowed, then really no one should be in grow shops. I know, some folks grow vegetables and other plants. But lets be honest, home growing of MJ is the biggest chunk f the business.
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Depends, as long as you're not a gangster using the store as a front to launder money or you're doing other things to get on the RCMP radar most stores were/are ok in the prohibition state. Nothing in the store is illegal, the authorities can assume what ever they want but in Canada there's a slim change of getting charged with conspiracy, unlike in the US.

Politicians well certain MLA's tried to introduce laws to restrict the sale of bulbs and ballasts to licenses electricians only but all that would have done is make people go to Alberta, load up on bulbs and ballasts and now instead of $35 per 1000W they'd be selling them for $100 making $65 profit more lol


The MMAR was good for small business and the mmpr is good for select golden blow job big business.
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MMAR was good when it was harder to get a license from a doctor before the idiots with 200-1000's lighters started to pop up left and right. These guys had tons money but they would never have grown with out the license. So they used their buddies to grows for them while they financed the grows. I can tell you one thing it wasn't organized crime making the MMAR go out of control that side was tiny compared to the people who were from the outside of the lifestyle abusing the hell out of the system. The "organized crime" people wanted to keep it down for a long term run while obviously using the license for a get out of jail free card BUT they knew that over saturation was bad for business.


I shop at independent Canadian owned business whenever I can ......but it's getting harder all the time.
I respect you Canna and wish you luck and good support in the shop. Maybe if your comfortable you could mention your shop and where it is. Maybe some folks on here nearer to your can send some business your way. If you don't wanna say I totally understand as that may be too personal.
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Thanks, there are some here who know :P
 
Flash63

Flash63

Well-Known Member
Re: hplc testing
We have our own research lab and chemist. Try MBI labs on Southern Van Island.

Quote:
Thats from from Chimera....hope it helps
 
M

Magenta Thumb

Well-Known Member
WHATFG said:
[snipped by Magenta Thumb] Are you in a lab magenta?
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I can't recall if I stated this when I first joined the board: PhD in Biochemistry. Most of my years on the analytical side have been spent using HPLC for protein analysis but I do know GC for small molecule (e.g. pesticide) work and also have small molecule experience for HPLC. I'm too old for UPLC.

You folks are spending too much time picking at the nits of HPLC vs GC and weight % of acids vs weight % of the decarboxylated forms when you should be asking what the LPs are using for their statistical release regimen. I am curious to know how many of the LP or their contract labs use USP <905>:
  • test 10 containers vs label claim
  • if they are too imprecise or inaccurate then test 20 more containers
  • if the data are still too loose, then the lot fails and may not be released
From what I can read in the MMPR, HC seems to be making them stick to +/-20% of label claim, which is wider than USP <905>'s usual target of trying to keep things within +/-15%. Note that for the high titre strains all y'all seem to get excited about, this means they will have the highest absolute allowable swing from container to container: so a 20% w/w label means the actual content could be 16 to 24% w/w. When I see label claims that have more than a single decimal point of % w/w, my experience dealing with HPLC and GC data take that as an indication that they simply had one to three samples assayed and are reporting the average generated by the lab: hardly a solid stats base for lot release.
 
CalyxCrusher

CalyxCrusher

Well-Known Member
Thanks Magenta,

It's also my understanding that HPLC has to be calibrated specifically for Cannabinoid testing. I would assume perhaps GC is the same way. That's the concerning part. This equipment can be used for testing for a wide variety of substances but unless it's calibrated to test cannabinoids specifically, you may as well be pissing into the wind. It'd be like using a PPM pen without first making sure it's calibrated properly first using a standard solution. I still hold my position on GC not being an effective method for measuring THC specifically, based on what I have seen and heard. HPLC is the method a vast majority of dispensaries in the US use to have their meds tested.
 
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