Shroom Liquid Culture

[MadDog607]

I want to start a series of teks to show you guys and gals how I grew booms. When I get a multiple spore syringe (ms syringe) from a vendor the first thing I do is make a bag of grain to inoculate (noc up) and I make a liquid culture ( Lc ). Liquid cultures are great because When you noc grain with it they colonize fast and they give you almost an endless supply of syringes worth of goodness. I learned this tek from another forum and I think I have learned some things from you and I want to spread my knowledge back into you. These teks are not about making money. It is about spreading knowledge and learning from what the end result is. Whatever your end result is it is sure to be someone elses beginning. So don't feel bad if it is bad and feal great if it is good. Eitherway you will definitely learn somthing about yourself. Lets get started.



Thing you will need:
1. mason jar
2. RTV silicon blue
3. 100% honey
4. water (tap is fine)
5. screw driver
6. Pressure cooker
7. marijuan (for smokin and shit)



Get the screw driver and make a hole in the lid of the jar:


Put RTV silicone on the top of the hole and the bottom of the hole. Make sure you get the shards of metal on the bottom. Let the RTV dry for a day. Smoke a bowel while you wait.




When the RTV is dry fill the jar up to the top of the ball letters with water. Put 3 table spoons of honey in the jar. This is for quart jars. Put the top on and shake the shit out of it. I mean shake it till your balls hurt. Make sure there is no honey at the bottom of the jar. It should look like this:




Put tinfoil on top of the jars and give them ¼ twist off. This means tighten the lid and give it ¼ twist off. Put them in the pressure cooker fill 1/3 with water and cook at 15 psi for 90 minutes.




When they are done let the pressure reduce to zero and open up the pressure cooker. Tighten the jars. This will create a vacuum seal. Let them sit and cool over night. Remove the tinfoil and noc em up.




The reason I wait to noc these jars up until I noc some bags is because I use the last of the syringe to noc the lc with. There will be a vacuum in the lc jar. So anything you stick in there will be sucked up. You can use a syringe with the plunger out to let air in but you risk contamination if you ask me. So just use the last of a syringe and save yourself the heartache.

After inoculating you shoulkd have a glob of myc in the bottom of the LC that looks like this:


If you guys need a tek on sanitary inoculation let me know. I figure we are past that, but if not just say something. I will give you my tek for that too. I just want you to succeed is all. My next tek will be for bags of grain to noc. Stay tuned my green cabbage brothers.

 

[MadDog607]

For some reason this thread does not look good on my puter. I'm just trying to help you guys grow booms. If you dont see the illustrations i'm sorry.

 

[frizzlegooch]

yeah we cant see, and it says to notify an administrator if you followed a link..
but i hope it gets fixed

 

[canndo]

The only problem here is that you still have a multiculture brew. This is fine for starting out or for small grows but liquid culture extends your ability to grow large amounts. It is allmost always best in large grows to have a monoculture. Your flushes are predictable and generally larger. You also know exactly what it is you are growing. Your method will work with tissue, with only a slight change. And again it will work just fine for those smaller grows. I don't want to step on things here but folks should know what it is they are getting.

 

[MadDog607]

Fixed it. Should be looking fine now.

Yes canndo you can throw some tissue in there from a shroom with specific traits you desire and clone it. This would give you almost identical shrooms giving you a bigger flush if you use the right shroom to clone with. I think this is where you are going with this. Just trying to keep it simple though. Just use a multiple spore syringe from a vendor. You will have an endless supply of perfectly good material to noc with. I have had dozens of these that have lasted me a long time. Under the right conditions you should be able to grow a shitload of booms with it. This is not for small grows either. Nothing small about what i do. I'll eventually post some pics of my boom boom room. Bring a toilet cuz your gonna shit.

 

[MadDog607]

yeah we cant see, and it says to notify an administrator if you followed a link..
but i hope it gets fixed

Do you still see the administrator notification? There should be no link here. I wrote this just for RIU.

 

[canndo]

Fixed it. Should be looking fine now.

Yes canndo you can throw some tissue in there from a shroom with specific traits you desire and clone it. This would give you almost identical shrooms giving you a bigger flush if you use the right shroom to clone with. I think this is where you are going with this. Just trying to keep it simple though. Just use a multiple spore syringe from a vendor. You will have an endless supply of perfectly good material to noc with. I have had dozens of these that have lasted me a long time. Under the right conditions you should be able to grow a shitload of booms with it. This is not for small grows either. Nothing small about what i do. I'll eventually post some pics of my boom boom room. Bring a toilet cuz your gonna shit.

I think you may miss the point of monoculture on a large scale. Of courses you can use mulitpsore on large scale, anything can be done with enough work and attention, however, growing large flushes are not dependant upon a lage individual mushroom, or the tendency of a paticular strain to bear large fruit, but the ability to orchestrate flushes is greatly enhanced when using a monoculture. I believe you have or do grow lots of mushrooms, but it is likely not as effiicient as it could be if you are using multispore cultures. I as well have grown large crops and consistantly got big yields.

I have even seen someone who managed to get a dried pound from a mass run of PF tec, hundreds of cakes, dozens and dozens of fruiting chambers, it worked, but was it the most efficient way? was it the way the mushroom ordinarily grows?

 

[MadDog607]

canndo I'm trying to keep this as simple as possible for people who are new to this. There is absolutely no problem with this tek like you said earlier. I haven't misled anyone in this tek either. It works great. Lets not over complicate this. I could of made a tek on cloning, monocultures, isolation but why? You have to crawl before you can walk. If people practice sterile procedures follow this tek and noc some grain they will have myc bottom line.

 

[canndo]

canndo I'm trying to keep this as simple as possible for people who are new to this. There is absolutely no problem with this tek like you said earlier. I haven't misled anyone in this tek either. It works great. Lets not over complicate this. I could of made a tek on cloning, monocultures, isolation but why? You have to crawl before you can walk. If people practice sterile procedures follow this tek and noc some grain they will have myc bottom line.

nothing wrong with simple, and now anyone who reads this can understand the tradeoffs.

 

[frizzlegooch]

Can you clarify what multispore means? is that like a mixed pack of seeds? you dont know the individual strains; as opposed to a monoculture being identifiable as one species?
kinda confused here :S
Good post tho, i hope you update soon

 

[MadDog607]

Multispore syringes are made from spore prints. They are the ones you usually get from vendors. The spores have all kinds of different genes. So when you grow with a multiple spore solution your shrooms will be different. Some will be short, tall, fat, skinny, some will grow faster than others and some will be aborts and not grow completely. A monoculture entails isolating desirable traits so when you grow with it your shrooms will have those traits and not the undesirable ones.

 

[technical dan]

a clone is a piece of fruit tissue reverted to myc and can still have multiple sets of genetics present. An isolate is a single set of (dikaryotic) genetics.
You should add a thing at the end of that post telling people to noc up a test jar/ cake or plate before a grain bag so they can see what has been growing in the LC

 

[MadDog607]

Yes you are right technical dan. Test jar to see if it is tammed? If you practice sterile technique and the syringe is good there should only be myc in the jar

 

[technical dan]

yep. if the syringe is good/ clean is a big if IME

 

[canndo]

OK, seems that the value of a monoculture is not being made clear here



Now look at these multispore dishes. Note that the indicidual colonies don't like each other, they form barriers between them and those barriers inhibit growth. On the plane, one will not overcome the other, the strongest will not "take over", this may or may not be the case in three dimensions but they will be competing regardless.



These are monocultures, they grow at a rapid and concerted rate, they are strong, vibrant and strongly rhyzomorphic. This is what you want your substrate to look like if at all possible.

 

[MadDog607]

No man! this thread is about simple lc tek okay. I think i was clear about the benefit of a monoculture earlier. Seriously canndo do you just like to read your own posts? Do you just like to sound intelligent? I swear i can post a pic of a shroom and I can count on you to come along and explain why it is not perfect. Why don't you make a long thread about monocultures and read it over and over again? You can even pretend and answer your own questions. All i wanted to do was show some people how to make a simple liquid culture that can be used to grow shrooms end of story. Then you come along and shit all over it like it will not be viable. Look I'm not trying to steal your thunder. I know this is your happy little home away from home, but damn! This is not a popularity contest. You can relax. No one is trying to knock you off of your thrown. Sorry if you feel that i am lashing out at you. It's just how i feel.

 

[Blakrex]

Great read MadDag. I was curious, what type of honey do you use for this? Would RAW honey work? Or are you using purified pasturized cane sugar honey? Also I was curious about the last picture. Is that right after injecting the syringe into the jar or does it slowly accumulate? Is the LC ready to use as soon as you finish the last step? Thanks for the great info! I think I have more questions, just trying to remember ha.

Edit: Oh! I remember now. Are there any particular websites you'd suggest for syringes? I just ran across thehawkseye.com, you know if they're a good source? Also how much of one of their 10cc syringes do you use to make one of these quart jars of LC? Do you mix the jar before pulling some with the syringe? Because it looks like it separates in the liquid.
-Hopefully that wasn't too much.

 

[MadDog607]

Great read MadDag. I was curious, what type of honey do you use for this? Would RAW honey work? Or are you using purified pasturized cane sugar honey? Also I was curious about the last picture. Is that right after injecting the syringe into the jar or does it slowly accumulate? Is the LC ready to use as soon as you finish the last step? Thanks for the great info! I think I have more questions, just trying to remember ha.

Edit: Oh! I remember now. Are there any particular websites you'd suggest for syringes? I just ran across thehawkseye.com, you know if they're a good source? Also how much of one of their 10cc syringes do you use to make one of these quart jars of LC? Do you mix the jar before pulling some with the syringe? Because it looks like it separates in the liquid.
-Hopefully that wasn't too much.

Great questions. I use 100% honey. You can find it at any grocery store. Last pic is after some time. You should have a little ball of myc in about 2 weeks. The LC in the last pic is about 1.5 years old in the pic. That LC has long came and gone. Wait till you see a nice ball of myc in the bottom of the LC before using it.

I have received spore syringes from sporeology.com and lilshopofspores.com. They were all viable. I would use 3ml of spore solution from a reputable vendor. I have made LC's with as little as 1ml before though. I would shake the jar before drawing it up. Otherwise it will just clog the syringe.

Thanks for ready this tek. If you have anymore questions feel free to ask.

 

[Blakrex]

Great questions. I use 100% honey. You can find it at any grocery store. Last pic is after some time. You should have a little ball of myc in about 2 weeks. The LC in the last pic is about 1.5 years old in the pic. That LC has long came and gone. Wait till you see a nice ball of myc in the bottom of the LC before using it.

I have received spore syringes from sporeology.com and lilshopofspores.com. They were all viable. I would use 3ml of spore solution from a reputable vendor. I have made LC's with as little as 1ml before though. I would shake the jar before drawing it up. Otherwise it will just clog the syringe.

Thanks for ready this tek. If you have anymore questions feel free to ask.

Aw okay. So what's the average shelf life of one of those quart jars? And about how long does it normally take before the jar is ready, or possibly the approximate size of mass at bottom (when ready)?

 

[MadDog607]

I would wait till the myc is a little smaller than the size of a golf ball. I have had some LC's for more than a year. Some go stagnant and seem to putter out. You will know this when your myc starts to look flat and disappear. Your jars or bags will start to stall on you or have weak myc growth in it. Then it's time to toss it, smoke a bowl and make another.